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The map label for this gene is lcfB [H]

Identifier: 86750372

GI number: 86750372

Start: 3730443

End: 3732047

Strand: Reverse

Name: lcfB [H]

Synonym: RPB_3261

Alternate gene names: 86750372

Gene position: 3732047-3730443 (Counterclockwise)

Preceding gene: 86750373

Following gene: 86750371

Centisome position: 70.0

GC content: 65.36

Gene sequence:

>1605_bases
ATGATCAATCTAGAAGCCACCGGCGGCGTGCCGGGACCGGGCCGGATCGGCCGGGTCGCGATCGGCGACATCCTGCGCAA
ATCGGCGCTGCGCTTTCCGGCGCGGATCGCGCTGACCGAGGGCGCGCGTCAGGTCAGCTACACCGAGCTCGAACGCGACG
CCAACCGCTTCGCCAACTATCTGGTGTCGCGCGGACTGAAGCCCGGCGACAAGATCTCGACGATCTGCAACAACTCGGTC
GAATTCGTCAAAGCGCTGTTCGGCATTCATCGCGCCGGGCTGGTCTGGGTGCCGATCAACACCATGCTCGGCCCCGACGA
CATGAGCTACATCCTCGATCATGCCGGGGTGAAGTTCGCGCTGATCGACGACAATCTGCACGGCCAGCCCGAGCGCCGCG
CCGCGCTGGAGAGCCGCGGCATCGCGCTGGTGGCGGTCGACCTCACCGGCAAGGCGAAAGAAGCCGGCCTGCGGAGCTTC
AACGAGCTGATCGACGGACACTCCGAGATCGAGCCGGAGGTCGCGTTCGACGATCGCGATCTGGCGATGATCATCTACAC
GTCCGGCACCACCTCGCGGCCGAAGGGGGCGATGCATTGCCACCTCGCGGTGACGATGGCGGTGATGAGCAACGCGATCG
AGCTGCAGCTCACCCGCAACGACGGCATCACCGGGCAGTTTCCGCTGTTCCACTGCGCCGCCCATGTGCTGCTGCTGAGC
TATCTCGCCGTCGGCGGCAAGATGGCGCTGATGCGCGGCTTCGACCCGGTCGCCTGCCTGGAAGCAATCCAGCGCGACAA
GCTCACCGTCTTCATCGGCCTGCCGCTGATGTATCAGGTGATCCTCGATCATCCGCGGCGCAAGGAATTCGACCTGTCGA
CGCTGCGCTGCTGCATCTACACTATGGCGCCGATGCCGCGGCCGCTGCTGGAGCGCGCGATTGCCGAGCTGTGTCCGAAT
TTCGTCCAGCCGTCCGGGCAGACCGAAATGTATCCGGCGACGACGATGTCGCAGCCGGACCGCCAGCTCGCTCGTTTCGG
CAATTACTGGGGCGAATCGTGCATGGTCAACGAGACCGCGATCATGGACGATGCCGGAAACCTGCTGCCGCCGGGTGAAG
TCGGCGAGCTGGTGCATCGCGGCCCCAACGTCATGCTCGGCTACTACAAGGATCCCGAGGCCACCGAGGCGGCGCGCAAA
TTCGGCTGGCACCACACCGGCGATCTGGCGCTGATCGACACCCACGGCGAGGTGCTGTTCGTCGACCGCAAGAAGGACAT
GATCAAATCCGGCGGCGAGAACGTCGCCTCGGTCAAGATCGAGGAGACGCTGCTGGCGCATCCGGCGGTGATGAACGCCG
CGGTGGTCGGCCTGCCGCATCCGCAATGGGGCGAGGCGGTGTCGGGCTTCGTCAAGCTCAAGCCGGGGGCTCAAGCGACC
GAGACCGAGATCATCGAGCACTGCCGCAAGACGCTCGGCGGCTTCCAGGTGCCGAAGCTGGTGCGCATCGTCGACGAGAT
GCCGATGACCGCCACCGGCAAGCTGCGCAAGGTCGAGCTGCGAAACCAGTTCACGGACTACTTCGCGATGGCGCAGACGG
GGTGA

Upstream 100 bases:

>100_bases
AAATCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCCAAGCAGCCTCAAGAGCTGCC
TCACCTCGAGGGAGTGAACC

Downstream 100 bases:

>100_bases
TTGTTGCGTCATTCCGGGGCGCACCGCGACGCGGTGCGAACCCGGAATCCCGACGTTCAGAGCACTTCGAGATTCCGGGT
TCGCGAACTGCGTTCGCGCC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MINLEATGGVPGPGRIGRVAIGDILRKSALRFPARIALTEGARQVSYTELERDANRFANYLVSRGLKPGDKISTICNNSV
EFVKALFGIHRAGLVWVPINTMLGPDDMSYILDHAGVKFALIDDNLHGQPERRAALESRGIALVAVDLTGKAKEAGLRSF
NELIDGHSEIEPEVAFDDRDLAMIIYTSGTTSRPKGAMHCHLAVTMAVMSNAIELQLTRNDGITGQFPLFHCAAHVLLLS
YLAVGGKMALMRGFDPVACLEAIQRDKLTVFIGLPLMYQVILDHPRRKEFDLSTLRCCIYTMAPMPRPLLERAIAELCPN
FVQPSGQTEMYPATTMSQPDRQLARFGNYWGESCMVNETAIMDDAGNLLPPGEVGELVHRGPNVMLGYYKDPEATEAARK
FGWHHTGDLALIDTHGEVLFVDRKKDMIKSGGENVASVKIEETLLAHPAVMNAAVVGLPHPQWGEAVSGFVKLKPGAQAT
ETEIIEHCRKTLGGFQVPKLVRIVDEMPMTATGKLRKVELRNQFTDYFAMAQTG

Sequences:

>Translated_534_residues
MINLEATGGVPGPGRIGRVAIGDILRKSALRFPARIALTEGARQVSYTELERDANRFANYLVSRGLKPGDKISTICNNSV
EFVKALFGIHRAGLVWVPINTMLGPDDMSYILDHAGVKFALIDDNLHGQPERRAALESRGIALVAVDLTGKAKEAGLRSF
NELIDGHSEIEPEVAFDDRDLAMIIYTSGTTSRPKGAMHCHLAVTMAVMSNAIELQLTRNDGITGQFPLFHCAAHVLLLS
YLAVGGKMALMRGFDPVACLEAIQRDKLTVFIGLPLMYQVILDHPRRKEFDLSTLRCCIYTMAPMPRPLLERAIAELCPN
FVQPSGQTEMYPATTMSQPDRQLARFGNYWGESCMVNETAIMDDAGNLLPPGEVGELVHRGPNVMLGYYKDPEATEAARK
FGWHHTGDLALIDTHGEVLFVDRKKDMIKSGGENVASVKIEETLLAHPAVMNAAVVGLPHPQWGEAVSGFVKLKPGAQAT
ETEIIEHCRKTLGGFQVPKLVRIVDEMPMTATGKLRKVELRNQFTDYFAMAQTG
>Mature_534_residues
MINLEATGGVPGPGRIGRVAIGDILRKSALRFPARIALTEGARQVSYTELERDANRFANYLVSRGLKPGDKISTICNNSV
EFVKALFGIHRAGLVWVPINTMLGPDDMSYILDHAGVKFALIDDNLHGQPERRAALESRGIALVAVDLTGKAKEAGLRSF
NELIDGHSEIEPEVAFDDRDLAMIIYTSGTTSRPKGAMHCHLAVTMAVMSNAIELQLTRNDGITGQFPLFHCAAHVLLLS
YLAVGGKMALMRGFDPVACLEAIQRDKLTVFIGLPLMYQVILDHPRRKEFDLSTLRCCIYTMAPMPRPLLERAIAELCPN
FVQPSGQTEMYPATTMSQPDRQLARFGNYWGESCMVNETAIMDDAGNLLPPGEVGELVHRGPNVMLGYYKDPEATEAARK
FGWHHTGDLALIDTHGEVLFVDRKKDMIKSGGENVASVKIEETLLAHPAVMNAAVVGLPHPQWGEAVSGFVKLKPGAQAT
ETEIIEHCRKTLGGFQVPKLVRIVDEMPMTATGKLRKVELRNQFTDYFAMAQTG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=28.2608695652174, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI187761345, Length=542, Percent_Identity=26.9372693726937, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI187761343, Length=542, Percent_Identity=26.9372693726937, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI38505220, Length=494, Percent_Identity=26.3157894736842, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI42544132, Length=493, Percent_Identity=25.5578093306288, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI58082049, Length=496, Percent_Identity=24.5967741935484, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI157311624, Length=497, Percent_Identity=24.1448692152917, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI157311622, Length=497, Percent_Identity=24.1448692152917, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI122937307, Length=498, Percent_Identity=26.5060240963855, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=24.21875, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI28416953, Length=529, Percent_Identity=23.062381852552, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI253970489, Length=566, Percent_Identity=25.2650176678445, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI8923896, Length=554, Percent_Identity=24.3682310469314, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=29.0262172284644, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI145693145, Length=522, Percent_Identity=27.3946360153257, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=28.1947261663286, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1786810, Length=511, Percent_Identity=26.2230919765166, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1790505, Length=521, Percent_Identity=23.0326295585413, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786801, Length=536, Percent_Identity=23.134328358209, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786529, Length=546, Percent_Identity=22.7106227106227, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=29.3478260869565, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=29.3478260869565, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=29.3478260869565, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17560308, Length=546, Percent_Identity=28.2051282051282, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17558820, Length=538, Percent_Identity=26.5799256505576, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=27.378640776699, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=502, Percent_Identity=25.4980079681275, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=499, Percent_Identity=25.0501002004008, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=502, Percent_Identity=26.2948207171315, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=530, Percent_Identity=23.2075471698113, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17551278, Length=551, Percent_Identity=25.5898366606171, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115534440, Length=540, Percent_Identity=24.8148148148148, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=509, Percent_Identity=22.7897838899804, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=352, Percent_Identity=26.7045454545455, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=389, Percent_Identity=24.4215938303342, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17541856, Length=233, Percent_Identity=27.0386266094421, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=343, Percent_Identity=29.4460641399417, Blast_Score=163, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6319264, Length=131, Percent_Identity=34.3511450381679, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24581924, Length=542, Percent_Identity=29.3357933579336, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18859661, Length=508, Percent_Identity=26.7716535433071, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355181, Length=518, Percent_Identity=25.2895752895753, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24666501, Length=480, Percent_Identity=25.8333333333333, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24666497, Length=480, Percent_Identity=25.8333333333333, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281366413, Length=480, Percent_Identity=25.8333333333333, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648255, Length=546, Percent_Identity=24.9084249084249, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648253, Length=545, Percent_Identity=24.4036697247706, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648260, Length=537, Percent_Identity=22.9050279329609, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI281365686, Length=394, Percent_Identity=24.8730964467005, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21358303, Length=524, Percent_Identity=21.9465648854962, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653035, Length=482, Percent_Identity=24.2738589211618, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI20130357, Length=487, Percent_Identity=23.6139630390144, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=528, Percent_Identity=22.5378787878788, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356947, Length=527, Percent_Identity=23.3396584440228, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161076582, Length=489, Percent_Identity=23.3128834355828, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648257, Length=540, Percent_Identity=22.5925925925926, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 58604; Mature: 58604

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLEATGGVPGPGRIGRVAIGDILRKSALRFPARIALTEGARQVSYTELERDANRFANY
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
LVSRGLKPGDKISTICNNSVEFVKALFGIHRAGLVWVPINTMLGPDDMSYILDHAGVKFA
HHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCEEE
LIDDNLHGQPERRAALESRGIALVAVDLTGKAKEAGLRSFNELIDGHSEIEPEVAFDDRD
EEECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCC
LAMIIYTSGTTSRPKGAMHCHLAVTMAVMSNAIELQLTRNDGITGQFPLFHCAAHVLLLS
EEEEEEECCCCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
YLAVGGKMALMRGFDPVACLEAIQRDKLTVFIGLPLMYQVILDHPRRKEFDLSTLRCCIY
HHHHCCHHHHHCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHH
TMAPMPRPLLERAIAELCPNFVQPSGQTEMYPATTMSQPDRQLARFGNYWGESCMVNETA
HHCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCE
IMDDAGNLLPPGEVGELVHRGPNVMLGYYKDPEATEAARKFGWHHTGDLALIDTHGEVLF
EECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCEEEEECCCCEEE
VDRKKDMIKSGGENVASVKIEETLLAHPAVMNAAVVGLPHPQWGEAVSGFVKLKPGAQAT
EECCHHHHHCCCCCEEEEEEHHHHHHCCHHHHEEEEECCCCCHHHHHCCEEEECCCCCHH
ETEIIEHCRKTLGGFQVPKLVRIVDEMPMTATGKLRKVELRNQFTDYFAMAQTG
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINLEATGGVPGPGRIGRVAIGDILRKSALRFPARIALTEGARQVSYTELERDANRFANY
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
LVSRGLKPGDKISTICNNSVEFVKALFGIHRAGLVWVPINTMLGPDDMSYILDHAGVKFA
HHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCEEE
LIDDNLHGQPERRAALESRGIALVAVDLTGKAKEAGLRSFNELIDGHSEIEPEVAFDDRD
EEECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCC
LAMIIYTSGTTSRPKGAMHCHLAVTMAVMSNAIELQLTRNDGITGQFPLFHCAAHVLLLS
EEEEEEECCCCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
YLAVGGKMALMRGFDPVACLEAIQRDKLTVFIGLPLMYQVILDHPRRKEFDLSTLRCCIY
HHHHCCHHHHHCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHH
TMAPMPRPLLERAIAELCPNFVQPSGQTEMYPATTMSQPDRQLARFGNYWGESCMVNETA
HHCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCE
IMDDAGNLLPPGEVGELVHRGPNVMLGYYKDPEATEAARKFGWHHTGDLALIDTHGEVLF
EECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCEEEEECCCCEEE
VDRKKDMIKSGGENVASVKIEETLLAHPAVMNAAVVGLPHPQWGEAVSGFVKLKPGAQAT
EECCHHHHHCCCCCEEEEEEHHHHHHCCHHHHEEEEECCCCCHHHHHCCEEEECCCCCHH
ETEIIEHCRKTLGGFQVPKLVRIVDEMPMTATGKLRKVELRNQFTDYFAMAQTG
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]