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The map label for this gene is yngE [H]

Identifier: 86750371

GI number: 86750371

Start: 3728651

End: 3730273

Strand: Reverse

Name: yngE [H]

Synonym: RPB_3260

Alternate gene names: 86750371

Gene position: 3730273-3728651 (Counterclockwise)

Preceding gene: 86750372

Following gene: 86750370

Centisome position: 69.96

GC content: 65.68

Gene sequence:

>1623_bases
ATGGCAATCATCGAATCCACCGTCGCGCCGGGGAGCGAAGTCTTCAAGAGCAACCGCGACGGCATGCTGGCGCTGATCGA
ACGCATGCGGATGCTGGAGGATCGTGCCCGGATCGCTTCCGCCGCATCCTCGGAGCGTTTCCATAAGCGCGGACAATTGC
TGCCGCGCGAACGCGTCGCGCTGGTGCTCGATCCCGGAACGCCGTTCCTGGAACTCTCGACGCTGGCCGGCTACATGTTC
GACACGGCGAATGCCGAGAAGAGTGTTCCGGGTGGCGGGGTGATCGGCGGCATCGGCTACGTCGCCGGCGTTCGCTGCAT
GATCAGCGCCAACGATTCCGGCATCGACGCCGGGGCGCTGCAGCCTTACGGGCTCGACAAGACGCTGCGCATCCAGGAAC
TGGCGCTGGAGAACAAGCTGCCCTTCGTGCAACTGGTCGAAAGCGCCGGCGCCAATCTGTTGCGCTATCGCGTCGAGGAC
TTCGTGCGCGGCGGCAATATTTTCCGCAATCTCGCATTGATGTCGGCGGCTGGACTGCCGGTGGTGACGGTGACGCACGG
CTCGTCGACCGCGGGTGGCGCGTATCAGACGGGATTGTCCGACTACATCGTCATGGTGCGCGGCCGGACGCGGGCGTTCC
TCGCCGGGCCGCCGCTGCTCAAGGCCGCGACCGGCGAGATCGCCACCGAGGAAGAGCTGGGCGGCGCCGAGATGCACACC
TCGGTCTCCGGGCTCGGCGACTACCTCGCGGAAGACGACCGCGATGCGCTGCGGATCGCGCGCGACATCATGGCCGGGCT
GCCGTGGGATCGCTCGGGACCGGGGCATGATCCCGCGACCTATCACCCGCCGCTGCACGATCCGGAAGAACTTCTCGGCA
TCATGCCGATGGATCACAAGCGCCCGGTCGATATGCGCCAGGTGATCGCGCGGATTGTCGACGATTCCGATTTCACCGAG
ATGTCGCCGAATTACGGTCCGGCGACGGTCGTCGGCCACGCCAAGATCGTCGGCCAGCCGATCGGCATCATCACCAACAA
CGGCCCGCTCGACCCCGCCGGCGCCAACAAGGCGACGCACTTCATTCAGGCGTGCTGCCAGTCGCGCACGCCGATCCTGT
ATCTCAACAACACCACCGGCTACATCGTCGGCAAGGCCTATGAGGAAGCCGGCATGATCAAGCACGGCTCGAAGATGATC
CAGGCGGTGACCTCCGCGTCGGTGCCGCAGATCACGATCTATTGCGGCGCCTCGTTCGGTGCCGGCAATTACGGCATGTG
CGGCCGCGGCTTCCATCCGCGGTTCTGCTTTTCCTGGCCGAACGCCAAGACCGCGGTGATGGGCGGTGAGCAGGCGGCGG
AGACCATGGCGATCGTCGCCGAAGCCGGCGCTGCACGAAAAGGCATTCCGGTCGACCGCGACAAGCTCGACCAGATGAAG
GGCGGCATCACCGCGGTGTTCAATGGCCAGATGGACGTGTTCGCGACTTCGGCGCGGATGCTCGACGACGGCGTGATCGA
CCCGCGTGACACCCGCGCCGTGCTGGCCGAGGTGCTGGCGATCTGTCGCGAAAGCGAGGCGCGCCAACCTCAGCGTATGC
AATTCGCGGTGTCCCGGGCATGA

Upstream 100 bases:

>100_bases
AGATTCCGGGTTCGCGAACTGCGTTCGCGCCCCGGAATGACGAACAAGAGGCGAGATTTCGGGGCGCTCTAATGCGTCTC
GGAACCACAAGGAACACCGA

Downstream 100 bases:

>100_bases
GTGCAACGCGCATGTTGAAATCCACACCTTTCACCAAAATCCTGATCGCCAATCGCGGCGAGATCGCGCTGCGCGTGATG
CGCACCGCACGCCGGATGGG

Product: propionyl-CoA carboxylase

Products: ADP; phosphate; (S)-methylmalonyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAIIESTVAPGSEVFKSNRDGMLALIERMRMLEDRARIASAASSERFHKRGQLLPRERVALVLDPGTPFLELSTLAGYMF
DTANAEKSVPGGGVIGGIGYVAGVRCMISANDSGIDAGALQPYGLDKTLRIQELALENKLPFVQLVESAGANLLRYRVED
FVRGGNIFRNLALMSAAGLPVVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPPLLKAATGEIATEEELGGAEMHT
SVSGLGDYLAEDDRDALRIARDIMAGLPWDRSGPGHDPATYHPPLHDPEELLGIMPMDHKRPVDMRQVIARIVDDSDFTE
MSPNYGPATVVGHAKIVGQPIGIITNNGPLDPAGANKATHFIQACCQSRTPILYLNNTTGYIVGKAYEEAGMIKHGSKMI
QAVTSASVPQITIYCGASFGAGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQAAETMAIVAEAGAARKGIPVDRDKLDQMK
GGITAVFNGQMDVFATSARMLDDGVIDPRDTRAVLAEVLAICRESEARQPQRMQFAVSRA

Sequences:

>Translated_540_residues
MAIIESTVAPGSEVFKSNRDGMLALIERMRMLEDRARIASAASSERFHKRGQLLPRERVALVLDPGTPFLELSTLAGYMF
DTANAEKSVPGGGVIGGIGYVAGVRCMISANDSGIDAGALQPYGLDKTLRIQELALENKLPFVQLVESAGANLLRYRVED
FVRGGNIFRNLALMSAAGLPVVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPPLLKAATGEIATEEELGGAEMHT
SVSGLGDYLAEDDRDALRIARDIMAGLPWDRSGPGHDPATYHPPLHDPEELLGIMPMDHKRPVDMRQVIARIVDDSDFTE
MSPNYGPATVVGHAKIVGQPIGIITNNGPLDPAGANKATHFIQACCQSRTPILYLNNTTGYIVGKAYEEAGMIKHGSKMI
QAVTSASVPQITIYCGASFGAGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQAAETMAIVAEAGAARKGIPVDRDKLDQMK
GGITAVFNGQMDVFATSARMLDDGVIDPRDTRAVLAEVLAICRESEARQPQRMQFAVSRA
>Mature_539_residues
AIIESTVAPGSEVFKSNRDGMLALIERMRMLEDRARIASAASSERFHKRGQLLPRERVALVLDPGTPFLELSTLAGYMFD
TANAEKSVPGGGVIGGIGYVAGVRCMISANDSGIDAGALQPYGLDKTLRIQELALENKLPFVQLVESAGANLLRYRVEDF
VRGGNIFRNLALMSAAGLPVVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPPLLKAATGEIATEEELGGAEMHTS
VSGLGDYLAEDDRDALRIARDIMAGLPWDRSGPGHDPATYHPPLHDPEELLGIMPMDHKRPVDMRQVIARIVDDSDFTEM
SPNYGPATVVGHAKIVGQPIGIITNNGPLDPAGANKATHFIQACCQSRTPILYLNNTTGYIVGKAYEEAGMIKHGSKMIQ
AVTSASVPQITIYCGASFGAGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQAAETMAIVAEAGAARKGIPVDRDKLDQMKG
GITAVFNGQMDVFATSARMLDDGVIDPRDTRAVLAEVLAICRESEARQPQRMQFAVSRA

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG4799

COG function: function code I; Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI11545863, Length=512, Percent_Identity=44.7265625, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI119943100, Length=519, Percent_Identity=28.7090558766859, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI295821216, Length=539, Percent_Identity=27.8293135435993, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI310133460, Length=140, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552936, Length=523, Percent_Identity=43.5946462715105, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25147362, Length=491, Percent_Identity=28.9205702647658, Blast_Score=212, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI25147359, Length=491, Percent_Identity=28.9205702647658, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24586065, Length=531, Percent_Identity=41.6195856873823, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000022
- InterPro:   IPR011763
- InterPro:   IPR011762 [H]

Pfam domain/function: PF01039 Carboxyl_trans [H]

EC number: 6.4.1.3

Molecular weight: Translated: 57713; Mature: 57582

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50980 COA_CT_NTER ; PS50989 COA_CT_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIESTVAPGSEVFKSNRDGMLALIERMRMLEDRARIASAASSERFHKRGQLLPRERVA
CCEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LVLDPGTPFLELSTLAGYMFDTANAEKSVPGGGVIGGIGYVAGVRCMISANDSGIDAGAL
EEECCCCCCEEHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCEEEEEECCCCCCCCCCC
QPYGLDKTLRIQELALENKLPFVQLVESAGANLLRYRVEDFVRGGNIFRNLALMSAAGLP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
VVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPPLLKAATGEIATEEELGGAEMHT
EEEEECCCCCCCCHHHCCCCEEEEEEECCCEEEECCCCCHHHCCCCCCCHHHCCCHHHHH
SVSGLGDYLAEDDRDALRIARDIMAGLPWDRSGPGHDPATYHPPLHDPEELLGIMPMDHK
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
RPVDMRQVIARIVDDSDFTEMSPNYGPATVVGHAKIVGQPIGIITNNGPLDPAGANKATH
CCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHEECCCEEEEECCCCCCCCCCCHHHH
FIQACCQSRTPILYLNNTTGYIVGKAYEEAGMIKHGSKMIQAVTSASVPQITIYCGASFG
HHHHHHCCCCCEEEEECCCCEEEEECHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
AGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQAAETMAIVAEAGAARKGIPVDRDKLDQMK
CCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHC
GGITAVFNGQMDVFATSARMLDDGVIDPRDTRAVLAEVLAICRESEARQPQRMQFAVSRA
CCEEEEECCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEECCC
>Mature Secondary Structure 
AIIESTVAPGSEVFKSNRDGMLALIERMRMLEDRARIASAASSERFHKRGQLLPRERVA
CEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LVLDPGTPFLELSTLAGYMFDTANAEKSVPGGGVIGGIGYVAGVRCMISANDSGIDAGAL
EEECCCCCCEEHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCEEEEEECCCCCCCCCCC
QPYGLDKTLRIQELALENKLPFVQLVESAGANLLRYRVEDFVRGGNIFRNLALMSAAGLP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
VVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPPLLKAATGEIATEEELGGAEMHT
EEEEECCCCCCCCHHHCCCCEEEEEEECCCEEEECCCCCHHHCCCCCCCHHHCCCHHHHH
SVSGLGDYLAEDDRDALRIARDIMAGLPWDRSGPGHDPATYHPPLHDPEELLGIMPMDHK
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
RPVDMRQVIARIVDDSDFTEMSPNYGPATVVGHAKIVGQPIGIITNNGPLDPAGANKATH
CCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHEECCCEEEEECCCCCCCCCCCHHHH
FIQACCQSRTPILYLNNTTGYIVGKAYEEAGMIKHGSKMIQAVTSASVPQITIYCGASFG
HHHHHHCCCCCEEEEECCCCEEEEECHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
AGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQAAETMAIVAEAGAARKGIPVDRDKLDQMK
CCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHC
GGITAVFNGQMDVFATSARMLDDGVIDPRDTRAVLAEVLAICRESEARQPQRMQFAVSRA
CCEEEEECCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; propanoyl-CoA; HCO3-

Specific reaction: ATP + propanoyl-CoA + HCO3- = ADP + phosphate + (S)-methylmalonyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9387222 [H]