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The map label for this gene is rsmB [H]

Identifier: 86750301

GI number: 86750301

Start: 3648054

End: 3649358

Strand: Reverse

Name: rsmB [H]

Synonym: RPB_3190

Alternate gene names: 86750301

Gene position: 3649358-3648054 (Counterclockwise)

Preceding gene: 86750302

Following gene: 86750300

Centisome position: 68.45

GC content: 72.18

Gene sequence:

>1305_bases
ATGACCCCCGCCGCACGGCTGTCTGCAGCCATCGACCTGATCGCCACCATCGACGGCCAGCGCATTCCTGCCGCCAAGGC
CCTGAAAGAGTGGGGCACGGCGCATCGCTACGCCGGCTCCGGCGATCGCTCTGCGATCGCGGGCCTGGTCTGGGATGTGC
TGCGCCGCCGCGCCTCCAGCGCCTTCCTGATGGACGACGACAGCGCCCGCGCCCGGGTGCTCGGCATGCTCAGGATCGAG
CGCGGCCTGACGACGGAGGCGATCGCGGCGCTGTGTGACGGCGGCCGGTTCGCGCCGGAGCCTTTGACCGACGCCGAACA
CGCCGCCTTGTCCGGGCGGACGCTCGACGCCGCGCCGCCGCATGTCGCCGGCGATTATCCGGAATGGCTCGACGGCGCGC
TGGCCGAGGTGTTCGGCGACGAGCGCGTCGCCGAGGCCGCCGCCATGGCAAGCCGCGCGCCGCTCGATCTGCGCGTCAAT
ACGCTGAAGGGCAAGCCCGACAAGGTGCTGGCCTCGCTCGCGCATCTCGGCGCCAGGGCGACGCCGTGGTCGCCGCTGGG
GCTGCGCATCGAGCTCGGCGCCGACGCCCGCAATCCCGGGGTGCAGGCCGAGCCGGATTTCATCAAGGGCGCCATCGAGG
TGCAGGACGAGGGCTCGCAGCTCGCGGCGCTGCTGTCGGCCGCCAAGCCCGGCGAGCAGGTGATCGACCTCTGCGCCGGC
GCCGGCGGCAAGACGCTGGCGCTAGCGGCGATGATGCAGGGCAAGGGCCGGCTGATCGCCACCGATTCCGACAAGCGCCA
GCTCGCGCCGATCCACGAGCGGCTGTCGCGCGCCGGCGTGCATAATTGCGACATCCGGGCTCCGAAGGGGCCGGCCGATA
CCCTGTCCGACATCCACGCCTCCGCCGATCTGGTGCTGGTCGACGCGCCGTGCACCGGCACCGGCACCTGGCGCCGCAAC
CCCGACGCCAAATGGCGGATGCGCCCCGGTGCGCTGGAAATCCGGCTGAAGGACCAGGCCGAGGTGCTGGCCCGCGCCGC
CGCGCTGGTGAAGCCCGGCGGCCGGATCGCCTATGTGACCTGCTCGGTGCTGCCGGCGGAGAACGGCGCGCAGATCAAGG
CGTTCACCGCCGCGCACCCGGACTTCGCCGTGACCCCGCCGGCCGAGGTCGCCGCCACGCTGTGGGACAAGGCCGAGGCC
TTCCTGGCCGCCACAAGAACCTCTTCCGAGGGGCTCTTGATGACCCCGCGCAAGACCGGGACGGACGGGTTTTTCGTGTC
GGTGCTGCGCAAGGCTGCGGCTTGA

Upstream 100 bases:

>100_bases
CAGGCGCGCTGGTGCTGCTGGCGATGTGGGTCTATTTCGCGCTGCGCATGCTGCTGCTGATCTGATATCCCTCGCGCCCC
TCGACGATTGAAAGACCGCA

Downstream 100 bases:

>100_bases
ACGAGCCGTCACCCTGAGGTGCGACCGCCGGGCGCTTCGCGCGCGGCGGGAGCCTCGAAGGGCGACGGCGTCGCGGGCGA
AGAGGCCGCAGAACAGCATC

Product: RNA methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTPAARLSAAIDLIATIDGQRIPAAKALKEWGTAHRYAGSGDRSAIAGLVWDVLRRRASSAFLMDDDSARARVLGMLRIE
RGLTTEAIAALCDGGRFAPEPLTDAEHAALSGRTLDAAPPHVAGDYPEWLDGALAEVFGDERVAEAAAMASRAPLDLRVN
TLKGKPDKVLASLAHLGARATPWSPLGLRIELGADARNPGVQAEPDFIKGAIEVQDEGSQLAALLSAAKPGEQVIDLCAG
AGGKTLALAAMMQGKGRLIATDSDKRQLAPIHERLSRAGVHNCDIRAPKGPADTLSDIHASADLVLVDAPCTGTGTWRRN
PDAKWRMRPGALEIRLKDQAEVLARAAALVKPGGRIAYVTCSVLPAENGAQIKAFTAAHPDFAVTPPAEVAATLWDKAEA
FLAATRTSSEGLLMTPRKTGTDGFFVSVLRKAAA

Sequences:

>Translated_434_residues
MTPAARLSAAIDLIATIDGQRIPAAKALKEWGTAHRYAGSGDRSAIAGLVWDVLRRRASSAFLMDDDSARARVLGMLRIE
RGLTTEAIAALCDGGRFAPEPLTDAEHAALSGRTLDAAPPHVAGDYPEWLDGALAEVFGDERVAEAAAMASRAPLDLRVN
TLKGKPDKVLASLAHLGARATPWSPLGLRIELGADARNPGVQAEPDFIKGAIEVQDEGSQLAALLSAAKPGEQVIDLCAG
AGGKTLALAAMMQGKGRLIATDSDKRQLAPIHERLSRAGVHNCDIRAPKGPADTLSDIHASADLVLVDAPCTGTGTWRRN
PDAKWRMRPGALEIRLKDQAEVLARAAALVKPGGRIAYVTCSVLPAENGAQIKAFTAAHPDFAVTPPAEVAATLWDKAEA
FLAATRTSSEGLLMTPRKTGTDGFFVSVLRKAAA
>Mature_433_residues
TPAARLSAAIDLIATIDGQRIPAAKALKEWGTAHRYAGSGDRSAIAGLVWDVLRRRASSAFLMDDDSARARVLGMLRIER
GLTTEAIAALCDGGRFAPEPLTDAEHAALSGRTLDAAPPHVAGDYPEWLDGALAEVFGDERVAEAAAMASRAPLDLRVNT
LKGKPDKVLASLAHLGARATPWSPLGLRIELGADARNPGVQAEPDFIKGAIEVQDEGSQLAALLSAAKPGEQVIDLCAGA
GGKTLALAAMMQGKGRLIATDSDKRQLAPIHERLSRAGVHNCDIRAPKGPADTLSDIHASADLVLVDAPCTGTGTWRRNP
DAKWRMRPGALEIRLKDQAEVLARAAALVKPGGRIAYVTCSVLPAENGAQIKAFTAAHPDFAVTPPAEVAATLWDKAEAF
LAATRTSSEGLLMTPRKTGTDGFFVSVLRKAAA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=253, Percent_Identity=30.8300395256917, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI76150623, Length=253, Percent_Identity=30.8300395256917, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI270288818, Length=300, Percent_Identity=28.6666666666667, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI8922322, Length=300, Percent_Identity=28.6666666666667, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI23199998, Length=300, Percent_Identity=28.6666666666667, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI270288816, Length=300, Percent_Identity=28.6666666666667, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI32698918, Length=184, Percent_Identity=29.8913043478261, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367212, Length=309, Percent_Identity=31.3915857605178, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081985, Length=294, Percent_Identity=29.9319727891156, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=260, Percent_Identity=30.3846153846154, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25143471, Length=175, Percent_Identity=34.8571428571429, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=300, Percent_Identity=28.6666666666667, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=181, Percent_Identity=32.0441988950276, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI22024126, Length=231, Percent_Identity=31.1688311688312, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355201, Length=221, Percent_Identity=28.5067873303167, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356579, Length=238, Percent_Identity=26.890756302521, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 45545; Mature: 45414

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAARLSAAIDLIATIDGQRIPAAKALKEWGTAHRYAGSGDRSAIAGLVWDVLRRRASS
CCCHHHHHHHHHHHEECCCCCCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHCCC
AFLMDDDSARARVLGMLRIERGLTTEAIAALCDGGRFAPEPLTDAEHAALSGRTLDAAPP
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCC
HVAGDYPEWLDGALAEVFGDERVAEAAAMASRAPLDLRVNTLKGKPDKVLASLAHLGARA
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCC
TPWSPLGLRIELGADARNPGVQAEPDFIKGAIEVQDEGSQLAALLSAAKPGEQVIDLCAG
CCCCCCCEEEEECCCCCCCCCCCCHHHHCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHC
AGGKTLALAAMMQGKGRLIATDSDKRQLAPIHERLSRAGVHNCDIRAPKGPADTLSDIHA
CCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHC
SADLVLVDAPCTGTGTWRRNPDAKWRMRPGALEIRLKDQAEVLARAAALVKPGGRIAYVT
CCCEEEEECCCCCCCCCCCCCCCCEEECCCEEEEEECHHHHHHHHHHHHHCCCCEEEEEE
CSVLPAENGAQIKAFTAAHPDFAVTPPAEVAATLWDKAEAFLAATRTSSEGLLMTPRKTG
EEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCC
TDGFFVSVLRKAAA
CCHHHHHHHHHHCC
>Mature Secondary Structure 
TPAARLSAAIDLIATIDGQRIPAAKALKEWGTAHRYAGSGDRSAIAGLVWDVLRRRASS
CCHHHHHHHHHHHEECCCCCCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHCCC
AFLMDDDSARARVLGMLRIERGLTTEAIAALCDGGRFAPEPLTDAEHAALSGRTLDAAPP
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCC
HVAGDYPEWLDGALAEVFGDERVAEAAAMASRAPLDLRVNTLKGKPDKVLASLAHLGARA
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCC
TPWSPLGLRIELGADARNPGVQAEPDFIKGAIEVQDEGSQLAALLSAAKPGEQVIDLCAG
CCCCCCCEEEEECCCCCCCCCCCCHHHHCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHC
AGGKTLALAAMMQGKGRLIATDSDKRQLAPIHERLSRAGVHNCDIRAPKGPADTLSDIHA
CCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHC
SADLVLVDAPCTGTGTWRRNPDAKWRMRPGALEIRLKDQAEVLARAAALVKPGGRIAYVT
CCCEEEEECCCCCCCCCCCCCCCCEEECCCEEEEEECHHHHHHHHHHHHHCCCCEEEEEE
CSVLPAENGAQIKAFTAAHPDFAVTPPAEVAATLWDKAEAFLAATRTSSEGLLMTPRKTG
EEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCC
TDGFFVSVLRKAAA
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA