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The map label for this gene is guaA

Identifier: 86750300

GI number: 86750300

Start: 3646204

End: 3647826

Strand: Reverse

Name: guaA

Synonym: RPB_3189

Alternate gene names: 86750300

Gene position: 3647826-3646204 (Counterclockwise)

Preceding gene: 86750301

Following gene: 86750298

Centisome position: 68.42

GC content: 67.28

Gene sequence:

>1623_bases
ATGACAGCCCCCAGCACACCCTCCGCTTCGTCCGTCGTCCCCTCCGGCGCCGACACCTCGCCGCATGTCGCAGCGATCCA
CGAGAAGATCCTGATCGTCGATTTCGGCTCGCAGGTCACCCAGCTGATCGCCCGCCGCGTCCGCGAGGAGGGCGTCTATT
CCGAGATCGTGCCGTTCCAGAAGGCGGAGGCCGCCTTCGCCGAGATGAAGCCGAAGGCCGTGATCCTGTCCGGCGGCCCG
GCCTCGGTGCTCGACGCCGACGCGCCCGCTGCGCCGATGGCGATCCTCGAGGCCGGCGTGCCGGTGCTCGGGATCTGCTA
CGGTGAACAGACGCTGGCCAAGCAGCTCGGCGGCACGGTCGAAGCCGGCCATCACCGCGAATTCGGCCGCGCCACGATCG
AGATCACCGACGACTGCGCGCTGTTCGACGGCGTCTGGCAGAAGGGCGGCACATACGACGTCTGGATGAGCCATGGCGAC
CGCGTCACCAAGCTGCCCGACGGCTTCCGCGGCGTCGCCAAGGCGCCGGGCTCGCCGATCTCGGTGATCGCCGACGACAA
ACGCAAGTTCTACGCGACGCAGTTCCATCTCGAAGTGGTGCACACGCCGGACGGCGCGAAAATCCTGCGCAACTTCGTCC
GCAAGGTCGCGGGGCTGACCGGCGACTGGACGATGCGCGCCTTCCGCGAGGAGGCGATCGAGAAGATCCGCGCGCAGGTC
GGCACCGGCAAGGTGATCTGCGGCCTGTCCGGCGGCGTCGACTCGGCGGTCGCCGCGGTGCTGATCCATGAGGCGATCGG
CGATCAGCTCACCTGCGTGTTCGTCGACCACGGCATGCTGCGCAAGGACGAGGCCAAGACCGTCGTCGACCTGTTCCGGC
ATCACTACAACATCCCGCTGGTGCATGTGGACGCGTCGGAGACCTTCCTCGGCGCGCTCAGCGGCGTTACCGATCCGGAG
CAGAAGCGCAAGATCATCGGCAAGCTGTTCATCGACGTGTTCGACGCCGAGGCCAAGGTCGTCGGCGGCGCTGATTACTT
GGCGCAAGGCACGCTGTATCCCGACGTGATCGAGAGCGTGTCGTTCACCGGCGGCCCGTCGGTGACGATCAAGTCGCACC
ACAATGTCGGCGGCCTGCCCGAACGCATGAACATGAAGCTGGTCGAACCCTTGCGCGAATTGTTCAAGGACGAGGTGAGG
GCGCTCGGCCGCGAGCTCGGCCTGCCGGAGATTTTCGTCGGCCGCCACCCGTTCCCGGGCCCGGGCCTGGCCATCCGCTG
CCCCGGCGAGATCACCGCCGAGAAGCTCGACATCCTGCGCAACGCCGACGCGGTGTATATCGACCAGATCCGCAAGGCCG
GCCTCTACGACGCGATCTGGCAGGCCTTCGCGGTGCTGCTGCCGGTCAAGACCGTCGGCGTGATGGGCGACGGCCGCACC
TACGAGTACGTCGTCGGCCTGCGCGCCGTGACCTCGACCGACGGCATGACGGCCGATTACTACCCGTTCGACATGGCCTT
CCTGGGCGCCACCGCGACCCGGATCATCAACGAGGTCAAGGGCGTCAACCGCGTGGTCTACGACGTGACCAGCAAGCCGC
CGGGGACCATCGAGTGGGAGTGA

Upstream 100 bases:

>100_bases
GCCTCAGGATGACGGCGGGGGCGGGGCTGGCCTTGGACGTCGGCTTCGCATCCCCGTTTTCCCCGTTGCGAGCCGGGCTG
CGGTCGCGTATTTGCTGCCC

Downstream 100 bases:

>100_bases
TCCAGGTCCACCTGAGCGTAATCCGAAACGCGCCGCGGCACGACCCGACACGCTGAAAGATAGGGCCCTTCGTCTCTCAT
CGATCGGTGGTGATCGGTCG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MTAPSTPSASSVVPSGADTSPHVAAIHEKILIVDFGSQVTQLIARRVREEGVYSEIVPFQKAEAAFAEMKPKAVILSGGP
ASVLDADAPAAPMAILEAGVPVLGICYGEQTLAKQLGGTVEAGHHREFGRATIEITDDCALFDGVWQKGGTYDVWMSHGD
RVTKLPDGFRGVAKAPGSPISVIADDKRKFYATQFHLEVVHTPDGAKILRNFVRKVAGLTGDWTMRAFREEAIEKIRAQV
GTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGMLRKDEAKTVVDLFRHHYNIPLVHVDASETFLGALSGVTDPE
QKRKIIGKLFIDVFDAEAKVVGGADYLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVR
ALGRELGLPEIFVGRHPFPGPGLAIRCPGEITAEKLDILRNADAVYIDQIRKAGLYDAIWQAFAVLLPVKTVGVMGDGRT
YEYVVGLRAVTSTDGMTADYYPFDMAFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE

Sequences:

>Translated_540_residues
MTAPSTPSASSVVPSGADTSPHVAAIHEKILIVDFGSQVTQLIARRVREEGVYSEIVPFQKAEAAFAEMKPKAVILSGGP
ASVLDADAPAAPMAILEAGVPVLGICYGEQTLAKQLGGTVEAGHHREFGRATIEITDDCALFDGVWQKGGTYDVWMSHGD
RVTKLPDGFRGVAKAPGSPISVIADDKRKFYATQFHLEVVHTPDGAKILRNFVRKVAGLTGDWTMRAFREEAIEKIRAQV
GTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGMLRKDEAKTVVDLFRHHYNIPLVHVDASETFLGALSGVTDPE
QKRKIIGKLFIDVFDAEAKVVGGADYLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVR
ALGRELGLPEIFVGRHPFPGPGLAIRCPGEITAEKLDILRNADAVYIDQIRKAGLYDAIWQAFAVLLPVKTVGVMGDGRT
YEYVVGLRAVTSTDGMTADYYPFDMAFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE
>Mature_539_residues
TAPSTPSASSVVPSGADTSPHVAAIHEKILIVDFGSQVTQLIARRVREEGVYSEIVPFQKAEAAFAEMKPKAVILSGGPA
SVLDADAPAAPMAILEAGVPVLGICYGEQTLAKQLGGTVEAGHHREFGRATIEITDDCALFDGVWQKGGTYDVWMSHGDR
VTKLPDGFRGVAKAPGSPISVIADDKRKFYATQFHLEVVHTPDGAKILRNFVRKVAGLTGDWTMRAFREEAIEKIRAQVG
TGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGMLRKDEAKTVVDLFRHHYNIPLVHVDASETFLGALSGVTDPEQ
KRKIIGKLFIDVFDAEAKVVGGADYLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRA
LGRELGLPEIFVGRHPFPGPGLAIRCPGEITAEKLDILRNADAVYIDQIRKAGLYDAIWQAFAVLLPVKTVGVMGDGRTY
EYVVGLRAVTSTDGMTADYYPFDMAFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=553, Percent_Identity=37.7938517179023, Blast_Score=314, Evalue=1e-85,
Organism=Escherichia coli, GI1788854, Length=521, Percent_Identity=54.126679462572, Blast_Score=578, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71992717, Length=571, Percent_Identity=38.0035026269702, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71992710, Length=571, Percent_Identity=38.0035026269702, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI133901714, Length=571, Percent_Identity=38.0035026269702, Blast_Score=315, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6323873, Length=526, Percent_Identity=52.2813688212928, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI281365319, Length=550, Percent_Identity=36, Blast_Score=291, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_RHOP2 (Q2IV75)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486796.1
- ProteinModelPortal:   Q2IV75
- STRING:   Q2IV75
- GeneID:   3910990
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3189
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   RPAL316058:RPB_3189-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 58230; Mature: 58099

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 106-106 ACT_SITE 196-196 ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSTPSASSVVPSGADTSPHVAAIHEKILIVDFGSQVTQLIARRVREEGVYSEIVPFQ
CCCCCCCCCCCCCCCCCCCCCCCEEEECEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCH
KAEAAFAEMKPKAVILSGGPASVLDADAPAAPMAILEAGVPVLGICYGEQTLAKQLGGTV
HHHHHHHHCCCCEEEECCCCCHHCCCCCCCCHHHHHHCCCCEEEEEECHHHHHHHCCCCC
EAGHHREFGRATIEITDDCALFDGVWQKGGTYDVWMSHGDRVTKLPDGFRGVAKAPGSPI
CCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEECCCCCCCCHHHCCCCCCE
SVIADDKRKFYATQFHLEVVHTPDGAKILRNFVRKVAGLTGDWTMRAFREEAIEKIRAQV
EEEECCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGMLRKDEAKTVVDLFRHHYNIPL
CCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCE
VHVDASETFLGALSGVTDPEQKRKIIGKLFIDVFDAEAKVVGGADYLAQGTLYPDVIESV
EEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCHHHHHHC
SFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRALGRELGLPEIFVGRHPFPG
CCCCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PGLAIRCPGEITAEKLDILRNADAVYIDQIRKAGLYDAIWQAFAVLLPVKTVGVMGDGRT
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCE
YEYVVGLRAVTSTDGMTADYYPFDMAFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE
EEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TAPSTPSASSVVPSGADTSPHVAAIHEKILIVDFGSQVTQLIARRVREEGVYSEIVPFQ
CCCCCCCCCCCCCCCCCCCCCCEEEECEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCH
KAEAAFAEMKPKAVILSGGPASVLDADAPAAPMAILEAGVPVLGICYGEQTLAKQLGGTV
HHHHHHHHCCCCEEEECCCCCHHCCCCCCCCHHHHHHCCCCEEEEEECHHHHHHHCCCCC
EAGHHREFGRATIEITDDCALFDGVWQKGGTYDVWMSHGDRVTKLPDGFRGVAKAPGSPI
CCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEECCCCCCCCHHHCCCCCCE
SVIADDKRKFYATQFHLEVVHTPDGAKILRNFVRKVAGLTGDWTMRAFREEAIEKIRAQV
EEEECCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGMLRKDEAKTVVDLFRHHYNIPL
CCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCE
VHVDASETFLGALSGVTDPEQKRKIIGKLFIDVFDAEAKVVGGADYLAQGTLYPDVIESV
EEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCHHHHHHC
SFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRALGRELGLPEIFVGRHPFPG
CCCCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PGLAIRCPGEITAEKLDILRNADAVYIDQIRKAGLYDAIWQAFAVLLPVKTVGVMGDGRT
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCE
YEYVVGLRAVTSTDGMTADYYPFDMAFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE
EEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA