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The map label for this gene is appB [H]

Identifier: 86750195

GI number: 86750195

Start: 3512229

End: 3513197

Strand: Reverse

Name: appB [H]

Synonym: RPB_3081

Alternate gene names: 86750195

Gene position: 3513197-3512229 (Counterclockwise)

Preceding gene: 86750196

Following gene: 86750194

Centisome position: 65.89

GC content: 63.26

Gene sequence:

>969_bases
ATGAAACGTTCGATCGTGCGGCGACTGTTTCAGATCATCTCGCTCATCCTCGGCGTCATCGTGATGAATTTCACGCTCAT
CCAGATGGCGCCGGGATCGCTGCTCGACATCATGACGTCCGAGCAGCAGGTCACCGATCCGGCGACGTTGCAGCACCTCA
AGCATTTGTACGGCCTCGATCAGCCGGCGATCGTGCAGCTGCTGCGCTACACCTGGTCGGTGTTCAGCTTCGACCTCGGC
TATTCGTATCGCCAGAACGCGCCGGTGTTCGACATCATCATGGCGCAGCTGCCCTCGACGCTGCTGCTGATGCTGACGTC
GCTCGGGGTGGCGGTGACGCTCGGGATGATCGCCGGCGTCATCGCGTCGGTGAAGGTCAACACGGCCTGGGACGTGGTGA
TCTCACTGGCAGCGGTGTTCTTCTTCGCCGCGCCGACGTTCTGGCTCGGAATCATGCTGATCATCTTCTTCGCCGTACAT
CTCGATTGGCTGCCGGTTGGCGGCATGACCACGATCGGTGGGAAGTTCGGGCCGGTCGGGCAGGTGGTCGACGTGATCCG
CCACCTGATCCTGCCGGCGCTGTCGCTCGGCCTGTTCTACGCCGCGATCTACACGCGGGTGATGCGTGCCTCGATGCTCG
AGGTGTTCTCGCTCGACTTCGTCCGCACCGCGCGCGCCAAGGGCCTGAGCAAGACCCGCGTGATCCTGGCGCACGTCTTC
CGCAACGCGCTGCTGCCGGTCGTCACGCTGCTCGGCCTGCAGCTCGGGACTGTCCTCGGCGGCTCGGTGGTGATCGAGGC
GGTGTTCTCCTGGCCGGGCATCGGCAGCTTGATGCTCGATAGCGTGATGAGTCGCAATTACCCGGTCGTGTTGGGCGTCT
TCGTTCTGTCGGCGGTCGTCGTCGCCGTCGCCAATCTGCTGGTCGATCTTGCCTACTACCGGCTTGATCCGCGAATCCGG
AGCCGATGA

Upstream 100 bases:

>100_bases
TGTCGAAGAAGGTCAAGGGCGTCGGCAATCGCGCCGATATTTCGGCGGACAGCCTGTCGGACGTTTGGCTCGACGTCTGA
TTTGTAGAGGAGATGACGTG

Downstream 100 bases:

>100_bases
ACATGAAAGATCGATCCGCTTTTGCCGAGTTTGCCTTGCGCTTCGCCCGTAACCGCGGCGCGCTGCTCGGAATTATCATC
ATTGTCGTCGTGTTGGGACT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKRSIVRRLFQIISLILGVIVMNFTLIQMAPGSLLDIMTSEQQVTDPATLQHLKHLYGLDQPAIVQLLRYTWSVFSFDLG
YSYRQNAPVFDIIMAQLPSTLLLMLTSLGVAVTLGMIAGVIASVKVNTAWDVVISLAAVFFFAAPTFWLGIMLIIFFAVH
LDWLPVGGMTTIGGKFGPVGQVVDVIRHLILPALSLGLFYAAIYTRVMRASMLEVFSLDFVRTARAKGLSKTRVILAHVF
RNALLPVVTLLGLQLGTVLGGSVVIEAVFSWPGIGSLMLDSVMSRNYPVVLGVFVLSAVVVAVANLLVDLAYYRLDPRIR
SR

Sequences:

>Translated_322_residues
MKRSIVRRLFQIISLILGVIVMNFTLIQMAPGSLLDIMTSEQQVTDPATLQHLKHLYGLDQPAIVQLLRYTWSVFSFDLG
YSYRQNAPVFDIIMAQLPSTLLLMLTSLGVAVTLGMIAGVIASVKVNTAWDVVISLAAVFFFAAPTFWLGIMLIIFFAVH
LDWLPVGGMTTIGGKFGPVGQVVDVIRHLILPALSLGLFYAAIYTRVMRASMLEVFSLDFVRTARAKGLSKTRVILAHVF
RNALLPVVTLLGLQLGTVLGGSVVIEAVFSWPGIGSLMLDSVMSRNYPVVLGVFVLSAVVVAVANLLVDLAYYRLDPRIR
SR
>Mature_322_residues
MKRSIVRRLFQIISLILGVIVMNFTLIQMAPGSLLDIMTSEQQVTDPATLQHLKHLYGLDQPAIVQLLRYTWSVFSFDLG
YSYRQNAPVFDIIMAQLPSTLLLMLTSLGVAVTLGMIAGVIASVKVNTAWDVVISLAAVFFFAAPTFWLGIMLIIFFAVH
LDWLPVGGMTTIGGKFGPVGQVVDVIRHLILPALSLGLFYAAIYTRVMRASMLEVFSLDFVRTARAKGLSKTRVILAHVF
RNALLPVVTLLGLQLGTVLGGSVVIEAVFSWPGIGSLMLDSVMSRNYPVVLGVFVLSAVVVAVANLLVDLAYYRLDPRIR
SR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=38.3480825958702, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1787053, Length=322, Percent_Identity=39.1304347826087, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1789888, Length=321, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1787497, Length=321, Percent_Identity=33.3333333333333, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787761, Length=332, Percent_Identity=34.6385542168675, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788504, Length=359, Percent_Identity=28.133704735376, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787550, Length=252, Percent_Identity=30.5555555555556, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35298; Mature: 35298

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSIVRRLFQIISLILGVIVMNFTLIQMAPGSLLDIMTSEQQVTDPATLQHLKHLYGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCCHHHHHHHHHHHCCC
QPAIVQLLRYTWSVFSFDLGYSYRQNAPVFDIIMAQLPSTLLLMLTSLGVAVTLGMIAGV
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASVKVNTAWDVVISLAAVFFFAAPTFWLGIMLIIFFAVHLDWLPVGGMTTIGGKFGPVG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHH
QVVDVIRHLILPALSLGLFYAAIYTRVMRASMLEVFSLDFVRTARAKGLSKTRVILAHVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RNALLPVVTLLGLQLGTVLGGSVVIEAVFSWPGIGSLMLDSVMSRNYPVVLGVFVLSAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
VAVANLLVDLAYYRLDPRIRSR
HHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MKRSIVRRLFQIISLILGVIVMNFTLIQMAPGSLLDIMTSEQQVTDPATLQHLKHLYGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCCHHHHHHHHHHHCCC
QPAIVQLLRYTWSVFSFDLGYSYRQNAPVFDIIMAQLPSTLLLMLTSLGVAVTLGMIAGV
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASVKVNTAWDVVISLAAVFFFAAPTFWLGIMLIIFFAVHLDWLPVGGMTTIGGKFGPVG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHH
QVVDVIRHLILPALSLGLFYAAIYTRVMRASMLEVFSLDFVRTARAKGLSKTRVILAHVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RNALLPVVTLLGLQLGTVLGGSVVIEAVFSWPGIGSLMLDSVMSRNYPVVLGVFVLSAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
VAVANLLVDLAYYRLDPRIRSR
HHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]