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The map label for this gene is appA [H]

Identifier: 86750196

GI number: 86750196

Start: 3513218

End: 3514825

Strand: Reverse

Name: appA [H]

Synonym: RPB_3082

Alternate gene names: 86750196

Gene position: 3514825-3513218 (Counterclockwise)

Preceding gene: 86750197

Following gene: 86750195

Centisome position: 65.92

GC content: 61.88

Gene sequence:

>1608_bases
ATGACTAGCCACATCAACAGACGAGTGTTTCTGGGTACGTCCGCCCTTGCGGCGATCGCCGCCGGAACGTCACTTTGCTT
TCCACCTGCTGCACTCGCGCAGGAGAAGCCGAAGAAGGGCGGCGTGCTGGTAGCGACCTGGGGTGGCTTCGAGCCGCAGG
CCGTGTTCGTTCCCGGCGGCGGTGGCTCCAGCCCGCTGATCAGCTCGACCAAGATCCTGGAGCCGCTGCTGCGCCAGGAC
AGCCAGGCCGGCTTCCTTCCCGTGCTGGCCACCGAGGTCAAGCCTTCGGCCGATTTCAAGTCGTACGACATCGTGCTCCG
TAAAGGCGTGACGTGGCACGACGGCAAGCCGTTCACCGCAGACGACGTGGTCTTCAGCATCGAAAAATACTGGCTGCAGA
CGATCGCCAAGGCGGCGCTGAAGAATTTCTCCGGGGCCGAGGTGACCGAAGGCGGGGTGCGCGTGAGCTTCAAAGAGCCG
ACGCCGGAGTTCTTCTTCAAGTCGGTGCTGGCGACCTCGCTGGTCATTCCGAAGCACGTCTACGACGGCTCGGAGATCGT
TACCAACCCCGCCAATAACGCGCCGATCGGCACCGGTCCGTTCAAGTTCAAGCAGTGGGTGCGCGGCAGCCATATTGAAT
ATGCCGCCAACGACAAATATTGGGATGCGGGCAAGCCCTATCTCAACGGCCTGGTGATGCGCTACTGGCGCGACGCCGCA
TCGCGCACCGCGGCCTTGGAAGCCGACGAACTGCAGCTCGGCATCTTCAACCCGATCCCGACCCCGGACATCGACCGGCT
GTCCAAGTCCGGCAAGTTCGTCGCATCGAACGACGGCTATCTCGGCGCGGCCTGGGCTTCGACCATCGAATTCAACAGCC
GCCGTGATATCGTCAAGGACCCGGCCGTGCGCCGCGCACTACTCACCGCCATCGACCGGGCGACCATCTCGGACGTTGTG
TATTTCGGCCGCGCCAAGCCCGGCACGTCCTTCGTCAGCTCGACAAACCCGAAATTCTACAATCCGAACCTGCCGCGATA
CGAGTTCGACGCGAAGAAAGCCGCCAAGATGCTCGACGATGCCGGCTACCCGAAGAAGGGCAAGTCGCGCTTCAAGGTTC
ACTTGCTTGCGGCCGGCTGGTTCGAGGAGAACGGCAAGGTCGGCCAGTTCGTGAAGCAGAATCTGGAAGACATCGGCGTC
GAAGTGACGCTCACCGTGCCCGACCGAGCGACTTCGCTGAAGCAGATCTACGGCGATTACGACTACGACATCGCGCTGTC
GAACTACGCGGCGCGGGTCGAGCTGGTTCCCCAGCAGACCGACTACCTCAGCACGAGCGGGATTGTGAAGGGCGCGGCCT
TCCGCAACGCCACCGGCTATTCCAATCCCGAAGTCGACGACATCGTCGCCAAGATGTCGGTGGAGTCCGACGAAGCCAAG
CGGAAGGACTTGGCCTTCAAATTGCAAGAGATTGCAGCGCGAGATCTGCCGATCACCGTTCTGGTCGAACTGATCCCGAC
GACGATGATGTCGAAGAAGGTCAAGGGCGTCGGCAATCGCGCCGATATTTCGGCGGACAGCCTGTCGGACGTTTGGCTCG
ACGTCTGA

Upstream 100 bases:

>100_bases
GCGATCCCCGCGTCGGTCAACTGATCTTCAAATTATCCCCGCCGAGCGCTGCTCGGCGGCCAACTGACAACGTCCAACAG
TTAGGGATCGGAGATTCGAG

Downstream 100 bases:

>100_bases
TTTGTAGAGGAGATGACGTGATGAAACGTTCGATCGTGCGGCGACTGTTTCAGATCATCTCGCTCATCCTCGGCGTCATC
GTGATGAATTTCACGCTCAT

Product: twin-arginine translocation pathway signal

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTSHINRRVFLGTSALAAIAAGTSLCFPPAALAQEKPKKGGVLVATWGGFEPQAVFVPGGGGSSPLISSTKILEPLLRQD
SQAGFLPVLATEVKPSADFKSYDIVLRKGVTWHDGKPFTADDVVFSIEKYWLQTIAKAALKNFSGAEVTEGGVRVSFKEP
TPEFFFKSVLATSLVIPKHVYDGSEIVTNPANNAPIGTGPFKFKQWVRGSHIEYAANDKYWDAGKPYLNGLVMRYWRDAA
SRTAALEADELQLGIFNPIPTPDIDRLSKSGKFVASNDGYLGAAWASTIEFNSRRDIVKDPAVRRALLTAIDRATISDVV
YFGRAKPGTSFVSSTNPKFYNPNLPRYEFDAKKAAKMLDDAGYPKKGKSRFKVHLLAAGWFEENGKVGQFVKQNLEDIGV
EVTLTVPDRATSLKQIYGDYDYDIALSNYAARVELVPQQTDYLSTSGIVKGAAFRNATGYSNPEVDDIVAKMSVESDEAK
RKDLAFKLQEIAARDLPITVLVELIPTTMMSKKVKGVGNRADISADSLSDVWLDV

Sequences:

>Translated_535_residues
MTSHINRRVFLGTSALAAIAAGTSLCFPPAALAQEKPKKGGVLVATWGGFEPQAVFVPGGGGSSPLISSTKILEPLLRQD
SQAGFLPVLATEVKPSADFKSYDIVLRKGVTWHDGKPFTADDVVFSIEKYWLQTIAKAALKNFSGAEVTEGGVRVSFKEP
TPEFFFKSVLATSLVIPKHVYDGSEIVTNPANNAPIGTGPFKFKQWVRGSHIEYAANDKYWDAGKPYLNGLVMRYWRDAA
SRTAALEADELQLGIFNPIPTPDIDRLSKSGKFVASNDGYLGAAWASTIEFNSRRDIVKDPAVRRALLTAIDRATISDVV
YFGRAKPGTSFVSSTNPKFYNPNLPRYEFDAKKAAKMLDDAGYPKKGKSRFKVHLLAAGWFEENGKVGQFVKQNLEDIGV
EVTLTVPDRATSLKQIYGDYDYDIALSNYAARVELVPQQTDYLSTSGIVKGAAFRNATGYSNPEVDDIVAKMSVESDEAK
RKDLAFKLQEIAARDLPITVLVELIPTTMMSKKVKGVGNRADISADSLSDVWLDV
>Mature_534_residues
TSHINRRVFLGTSALAAIAAGTSLCFPPAALAQEKPKKGGVLVATWGGFEPQAVFVPGGGGSSPLISSTKILEPLLRQDS
QAGFLPVLATEVKPSADFKSYDIVLRKGVTWHDGKPFTADDVVFSIEKYWLQTIAKAALKNFSGAEVTEGGVRVSFKEPT
PEFFFKSVLATSLVIPKHVYDGSEIVTNPANNAPIGTGPFKFKQWVRGSHIEYAANDKYWDAGKPYLNGLVMRYWRDAAS
RTAALEADELQLGIFNPIPTPDIDRLSKSGKFVASNDGYLGAAWASTIEFNSRRDIVKDPAVRRALLTAIDRATISDVVY
FGRAKPGTSFVSSTNPKFYNPNLPRYEFDAKKAAKMLDDAGYPKKGKSRFKVHLLAAGWFEENGKVGQFVKQNLEDIGVE
VTLTVPDRATSLKQIYGDYDYDIALSNYAARVELVPQQTDYLSTSGIVKGAAFRNATGYSNPEVDDIVAKMSVESDEAKR
KDLAFKLQEIAARDLPITVLVELIPTTMMSKKVKGVGNRADISADSLSDVWLDV

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=309, Percent_Identity=29.4498381877023, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787052, Length=461, Percent_Identity=24.7288503253796, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082063, Length=493, Percent_Identity=19.6754563894523, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58404; Mature: 58273

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHINRRVFLGTSALAAIAAGTSLCFPPAALAQEKPKKGGVLVATWGGFEPQAVFVPGG
CCCCCCCEEEECHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCEEEEECC
GGSSPLISSTKILEPLLRQDSQAGFLPVLATEVKPSADFKSYDIVLRKGVTWHDGKPFTA
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEECCCCCCCH
DDVVFSIEKYWLQTIAKAALKNFSGAEVTEGGVRVSFKEPTPEFFFKSVLATSLVIPKHV
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCHHH
YDGSEIVTNPANNAPIGTGPFKFKQWVRGSHIEYAANDKYWDAGKPYLNGLVMRYWRDAA
CCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHH
SRTAALEADELQLGIFNPIPTPDIDRLSKSGKFVASNDGYLGAAWASTIEFNSRRDIVKD
HHHEECCCCCEEEEECCCCCCCCHHHHHCCCCEEECCCCEEEEHHHHEEECCCCCCCCCC
PAVRRALLTAIDRATISDVVYFGRAKPGTSFVSSTNPKFYNPNLPRYEFDAKKAAKMLDD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCEECCHHHHHHHHHC
AGYPKKGKSRFKVHLLAAGWFEENGKVGQFVKQNLEDIGVEVTLTVPDRATSLKQIYGDY
CCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCC
DYDIALSNYAARVELVPQQTDYLSTSGIVKGAAFRNATGYSNPEVDDIVAKMSVESDEAK
CEEEEECCCEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
RKDLAFKLQEIAARDLPITVLVELIPTTMMSKKVKGVGNRADISADSLSDVWLDV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TSHINRRVFLGTSALAAIAAGTSLCFPPAALAQEKPKKGGVLVATWGGFEPQAVFVPGG
CCCCCCEEEECHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCEEEEECC
GGSSPLISSTKILEPLLRQDSQAGFLPVLATEVKPSADFKSYDIVLRKGVTWHDGKPFTA
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEECCCCCCCH
DDVVFSIEKYWLQTIAKAALKNFSGAEVTEGGVRVSFKEPTPEFFFKSVLATSLVIPKHV
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCHHH
YDGSEIVTNPANNAPIGTGPFKFKQWVRGSHIEYAANDKYWDAGKPYLNGLVMRYWRDAA
CCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHH
SRTAALEADELQLGIFNPIPTPDIDRLSKSGKFVASNDGYLGAAWASTIEFNSRRDIVKD
HHHEECCCCCEEEEECCCCCCCCHHHHHCCCCEEECCCCEEEEHHHHEEECCCCCCCCCC
PAVRRALLTAIDRATISDVVYFGRAKPGTSFVSSTNPKFYNPNLPRYEFDAKKAAKMLDD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCEECCHHHHHHHHHC
AGYPKKGKSRFKVHLLAAGWFEENGKVGQFVKQNLEDIGVEVTLTVPDRATSLKQIYGDY
CCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCC
DYDIALSNYAARVELVPQQTDYLSTSGIVKGAAFRNATGYSNPEVDDIVAKMSVESDEAK
CEEEEECCCEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
RKDLAFKLQEIAARDLPITVLVELIPTTMMSKKVKGVGNRADISADSLSDVWLDV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]