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The map label for this gene is tgt [H]

Identifier: 86749986

GI number: 86749986

Start: 3267085

End: 3268227

Strand: Reverse

Name: tgt [H]

Synonym: RPB_2869

Alternate gene names: 86749986

Gene position: 3268227-3267085 (Counterclockwise)

Preceding gene: 86749987

Following gene: 86749984

Centisome position: 61.3

GC content: 68.85

Gene sequence:

>1143_bases
ATGAGCGTGCCGAACCATTTCGACCTGCTCGCGACCGACGGCGCGGCCCGCACCGGCCGGCTGACCACGCCGCATGGCGT
GGTGCGGACGCCGGCCTTCATGCCGGTCGGCACCGCGGGCGCGATGAAGGGGCTGCACTGGCGCGAGGTGCGCGACGCCG
GCGCCGATATCGTGCTCGGCAACACCTATCATCTGATGCTGCGCCCGGGTGCGGAGCGGATCGCGGCGCTCGGCGGCTTG
CAGCGCTTCACCACCTGGAACGGGCCGATGCTGACCGACAGCGGCGGCTTCCAGGTGATGTCGCTGGCGCAGCTCCGCAA
AGTCTCCGAGAAGGCGGTGAACTTCCGCTCGCATATCGACGGCGCCGCAATCGAATTGTCGCCGGAACGCGCCATCGAAG
TGCAGCTTCTGCTCGGCAGCGACATCGCGATGCAGCTCGACGAATGCGTCCGGCTGCCGGCGGAGCGCGCCGACATCGAG
CGCGCGATGCAACTGTCGCTGCGCTGGGCCGAGCGCTGCAAGCGCGCGTTCGAGACGGCGCCGGACGGCCACATGCTGTT
CGGCATCGTCCAGGGCGGCGACGTGCCGGAACTGCGCCACGCCAGCGCGCGCGGGCTGATCGAGCTCGGCTTCCACGGCT
ATGCGATCGGCGGCCTCGCGGTCGGCGAGCCGCAGGAGGTGATGCTTGCGATGATCGAGGAGGCGGCGCCGATCCTGCCG
GCCGACCGGCCGCGCTATCTGATGGGCGTCGGTACGCCCGAGGACATGCTCGAAGCGGTGGCGCGCGGCATCGACATGTT
CGACTGCGTGATGCCGACCCGCAACGGCCGCCACGGCGTGGCGTTCACCAGGTTCGGACCGGTCAATCTGCGCAACGCCC
GCCACGCCGACGATCCGCGCCCGCTGGACGAGGCGAGCGACTGGCCCTCGACCCGCAACTGCTCACGGGCCTATCTCCAT
CATCTGATCAAGTCGGGCGAAACGCTCGGCGCGATGCTGCTGTCGGAGATCAACATCGCGTACTATCAGCGGCTGATGCG
CGATATCCGCGAAGCGATCACGCGCGGCTCGTTCGAGACGTTCCGGATGCAGACCCGAGCCGACTGGGAGCAGGGCGATA
TCGCATCAAGAGACCCCGCATGA

Upstream 100 bases:

>100_bases
GCGGGCTGGAGACGATGCAGGCGGCCTACCGCCACGCCATCGAAACCGGCTATCGGTTCTATTCCTATGGCGACGCCAGC
CTGCTGTTCCGGGAGCCCAC

Downstream 100 bases:

>100_bases
CCGCGAGAAACGCAGCGCCGAGATCAGTTACAGGCGAAGCGTTTCATCGAATGCTTGGTTACAAAGCCATACCCGCAGGT
GTCGCAGGTCCAGAGATAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSVPNHFDLLATDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGLHWREVRDAGADIVLGNTYHLMLRPGAERIAALGGL
QRFTTWNGPMLTDSGGFQVMSLAQLRKVSEKAVNFRSHIDGAAIELSPERAIEVQLLLGSDIAMQLDECVRLPAERADIE
RAMQLSLRWAERCKRAFETAPDGHMLFGIVQGGDVPELRHASARGLIELGFHGYAIGGLAVGEPQEVMLAMIEEAAPILP
ADRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNGRHGVAFTRFGPVNLRNARHADDPRPLDEASDWPSTRNCSRAYLH
HLIKSGETLGAMLLSEINIAYYQRLMRDIREAITRGSFETFRMQTRADWEQGDIASRDPA

Sequences:

>Translated_380_residues
MSVPNHFDLLATDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGLHWREVRDAGADIVLGNTYHLMLRPGAERIAALGGL
QRFTTWNGPMLTDSGGFQVMSLAQLRKVSEKAVNFRSHIDGAAIELSPERAIEVQLLLGSDIAMQLDECVRLPAERADIE
RAMQLSLRWAERCKRAFETAPDGHMLFGIVQGGDVPELRHASARGLIELGFHGYAIGGLAVGEPQEVMLAMIEEAAPILP
ADRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNGRHGVAFTRFGPVNLRNARHADDPRPLDEASDWPSTRNCSRAYLH
HLIKSGETLGAMLLSEINIAYYQRLMRDIREAITRGSFETFRMQTRADWEQGDIASRDPA
>Mature_379_residues
SVPNHFDLLATDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGLHWREVRDAGADIVLGNTYHLMLRPGAERIAALGGLQ
RFTTWNGPMLTDSGGFQVMSLAQLRKVSEKAVNFRSHIDGAAIELSPERAIEVQLLLGSDIAMQLDECVRLPAERADIER
AMQLSLRWAERCKRAFETAPDGHMLFGIVQGGDVPELRHASARGLIELGFHGYAIGGLAVGEPQEVMLAMIEEAAPILPA
DRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNGRHGVAFTRFGPVNLRNARHADDPRPLDEASDWPSTRNCSRAYLHH
LIKSGETLGAMLLSEINIAYYQRLMRDIREAITRGSFETFRMQTRADWEQGDIASRDPA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=346, Percent_Identity=44.2196531791908, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI13375872, Length=259, Percent_Identity=25.8687258687259, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786607, Length=355, Percent_Identity=53.2394366197183, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=375, Percent_Identity=42.4, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI19920510, Length=347, Percent_Identity=45.821325648415, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41848; Mature: 41716

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPNHFDLLATDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGLHWREVRDAGADIVLG
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCEEEC
NTYHLMLRPGAERIAALGGLQRFTTWNGPMLTDSGGFQVMSLAQLRKVSEKAVNFRSHID
CEEEEEECCCHHHHHHHHCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GAAIELSPERAIEVQLLLGSDIAMQLDECVRLPAERADIERAMQLSLRWAERCKRAFETA
CCEEEECCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDGHMLFGIVQGGDVPELRHASARGLIELGFHGYAIGGLAVGEPQEVMLAMIEEAAPILP
CCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCEEECCEECCCHHHHHHHHHHHHCCCCC
ADRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNGRHGVAFTRFGPVNLRNARHADDPR
CCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
PLDEASDWPSTRNCSRAYLHHLIKSGETLGAMLLSEINIAYYQRLMRDIREAITRGSFET
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FRMQTRADWEQGDIASRDPA
HEEHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVPNHFDLLATDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGLHWREVRDAGADIVLG
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCEEEC
NTYHLMLRPGAERIAALGGLQRFTTWNGPMLTDSGGFQVMSLAQLRKVSEKAVNFRSHID
CEEEEEECCCHHHHHHHHCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GAAIELSPERAIEVQLLLGSDIAMQLDECVRLPAERADIERAMQLSLRWAERCKRAFETA
CCEEEECCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDGHMLFGIVQGGDVPELRHASARGLIELGFHGYAIGGLAVGEPQEVMLAMIEEAAPILP
CCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCEEECCEECCCHHHHHHHHHHHHCCCCC
ADRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNGRHGVAFTRFGPVNLRNARHADDPR
CCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
PLDEASDWPSTRNCSRAYLHHLIKSGETLGAMLLSEINIAYYQRLMRDIREAITRGSFET
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FRMQTRADWEQGDIASRDPA
HEEHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]