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The map label for this gene is queA [H]

Identifier: 86749987

GI number: 86749987

Start: 3268224

End: 3269378

Strand: Reverse

Name: queA [H]

Synonym: RPB_2870

Alternate gene names: 86749987

Gene position: 3269378-3268224 (Counterclockwise)

Preceding gene: 86749988

Following gene: 86749986

Centisome position: 61.32

GC content: 70.04

Gene sequence:

>1155_bases
ATGCCCGGGCTTGACCCGGGCATCCATCTTTCGAAGAGGATGGATCGCCGGATCAAGTCCGGCGATGACGGATACCGGAT
GCGCACCGACCTGTTCGACTTCGACCTGCCTGCCGAGAACATCGCGCTGCGGCCGATGAGCCCGCGCGATGCCGCGCGGA
TGCTGGTGGTGCGCCCCGGCGCGGCGCCGGAGGATCGCGTCGTGCGCGACCTGCCGGCGCTGCTGCAGCCCGGCGATCAG
CTCGTCGTCAACGATACGCGGGTGATCGCCGCGCAGCTCTCAGGCCGCCGGATCGGCCGCGCCACCGAGCCGAAGATCGA
GGCGACGCTGATCAAGCGGCTCGACGGCTCGCGCTGGCAGGCGCTGGTCAAGCCGGCGAAGAAGCTCGCCGAGGGCGACG
TCGTCCGCTTCGGCCATGACGGGCGGGTCTGTCTGCTCGGCCATCTCGACGCCACCGTCGAGGTCAAGGGCGAGGCCGGC
GAGGTGACGCTGGCGTTCTGCTTCCACGGGCCCGCGCTCGATCAGGCGATCGCCGAGCTCGGCGCGACGCCGCTGCCGCC
CTATATCGCGTCCAAGCGCGCGCCCGACGATCGGGACGCGGCCGACTATCAGACCATGTTCGCCGCCAACGAGGGCGCCG
TCGCGGCGCCGACCGCGGGGCTGCACTTCACGCCGGAACTCGACGCGGCACTCGCGGCGCGGGGCGTGACGGTGCACCGG
GTGACGCTGCATGTCGGGGCGGGGACGTTCCTGCCGGTGAAGACCGACGACACCGCCGAGCATCGGATGCACGCCGAATG
GGGCACGATCGGCCGTGACACCGCCGCGGCGCTGAACGCGGCGCGCGCGCAAGGCGGCCGCATCGTCGCGGTCGGCACCA
CCTCGCTGCGGCTGCTGGAAAGCGCGGCGCGCGACGACGGCACGATCGCGCCGTTCGCCGACGAAACCGCGATCTTCATC
ACGCCGGGCTACAAATTTCGTGCGGTCGACGTGCTGATGACCAATTTCCATCTGCCGCGGTCGACGCTGTTCATGCTGGT
GTCTGCGTTCTGCGGGCTGGAGACGATGCAGGCGGCCTACCGCCACGCCATCGAAACCGGCTATCGGTTCTATTCCTATG
GCGACGCCAGCCTGCTGTTCCGGGAGCCCACATGA

Upstream 100 bases:

>100_bases
GAACCCCGACAAGATCGTCAAGGCGACGATGGCCGCCGACGCGGCGGCTTGAGCTGCCGAGGTCGGCTCCTGACGCCGAC
GGCGCCACATAGAAGGTGTC

Downstream 100 bases:

>100_bases
GCGTGCCGAACCATTTCGACCTGCTCGCGACCGACGGCGCGGCCCGCACCGGCCGGCTGACCACGCCGCATGGCGTGGTG
CGGACGCCGGCCTTCATGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MPGLDPGIHLSKRMDRRIKSGDDGYRMRTDLFDFDLPAENIALRPMSPRDAARMLVVRPGAAPEDRVVRDLPALLQPGDQ
LVVNDTRVIAAQLSGRRIGRATEPKIEATLIKRLDGSRWQALVKPAKKLAEGDVVRFGHDGRVCLLGHLDATVEVKGEAG
EVTLAFCFHGPALDQAIAELGATPLPPYIASKRAPDDRDAADYQTMFAANEGAVAAPTAGLHFTPELDAALAARGVTVHR
VTLHVGAGTFLPVKTDDTAEHRMHAEWGTIGRDTAAALNAARAQGGRIVAVGTTSLRLLESAARDDGTIAPFADETAIFI
TPGYKFRAVDVLMTNFHLPRSTLFMLVSAFCGLETMQAAYRHAIETGYRFYSYGDASLLFREPT

Sequences:

>Translated_384_residues
MPGLDPGIHLSKRMDRRIKSGDDGYRMRTDLFDFDLPAENIALRPMSPRDAARMLVVRPGAAPEDRVVRDLPALLQPGDQ
LVVNDTRVIAAQLSGRRIGRATEPKIEATLIKRLDGSRWQALVKPAKKLAEGDVVRFGHDGRVCLLGHLDATVEVKGEAG
EVTLAFCFHGPALDQAIAELGATPLPPYIASKRAPDDRDAADYQTMFAANEGAVAAPTAGLHFTPELDAALAARGVTVHR
VTLHVGAGTFLPVKTDDTAEHRMHAEWGTIGRDTAAALNAARAQGGRIVAVGTTSLRLLESAARDDGTIAPFADETAIFI
TPGYKFRAVDVLMTNFHLPRSTLFMLVSAFCGLETMQAAYRHAIETGYRFYSYGDASLLFREPT
>Mature_383_residues
PGLDPGIHLSKRMDRRIKSGDDGYRMRTDLFDFDLPAENIALRPMSPRDAARMLVVRPGAAPEDRVVRDLPALLQPGDQL
VVNDTRVIAAQLSGRRIGRATEPKIEATLIKRLDGSRWQALVKPAKKLAEGDVVRFGHDGRVCLLGHLDATVEVKGEAGE
VTLAFCFHGPALDQAIAELGATPLPPYIASKRAPDDRDAADYQTMFAANEGAVAAPTAGLHFTPELDAALAARGVTVHRV
TLHVGAGTFLPVKTDDTAEHRMHAEWGTIGRDTAAALNAARAQGGRIVAVGTTSLRLLESAARDDGTIAPFADETAIFIT
PGYKFRAVDVLMTNFHLPRSTLFMLVSAFCGLETMQAAYRHAIETGYRFYSYGDASLLFREPT

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=361, Percent_Identity=44.3213296398892, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41564; Mature: 41433

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLDPGIHLSKRMDRRIKSGDDGYRMRTDLFDFDLPAENIALRPMSPRDAARMLVVRPG
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEEEEECCC
AAPEDRVVRDLPALLQPGDQLVVNDTRVIAAQLSGRRIGRATEPKIEATLIKRLDGSRWQ
CCCHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCHHHH
ALVKPAKKLAEGDVVRFGHDGRVCLLGHLDATVEVKGEAGEVTLAFCFHGPALDQAIAEL
HHHHHHHHHCCCCEEEECCCCCEEEEEECCEEEEEECCCCCEEEEEEECCHHHHHHHHHH
GATPLPPYIASKRAPDDRDAADYQTMFAANEGAVAAPTAGLHFTPELDAALAARGVTVHR
CCCCCCCHHHCCCCCCCCCCHHHHHEEECCCCCEECCCCCCEECCCHHHHHHHCCCEEEE
VTLHVGAGTFLPVKTDDTAEHRMHAEWGTIGRDTAAALNAARAQGGRIVAVGTTSLRLLE
EEEEECCCEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHH
SAARDDGTIAPFADETAIFITPGYKFRAVDVLMTNFHLPRSTLFMLVSAFCGLETMQAAY
HHCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHH
RHAIETGYRFYSYGDASLLFREPT
HHHHHHCCEEEEECCCEEEEECCC
>Mature Secondary Structure 
PGLDPGIHLSKRMDRRIKSGDDGYRMRTDLFDFDLPAENIALRPMSPRDAARMLVVRPG
CCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEEEEECCC
AAPEDRVVRDLPALLQPGDQLVVNDTRVIAAQLSGRRIGRATEPKIEATLIKRLDGSRWQ
CCCHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCHHHH
ALVKPAKKLAEGDVVRFGHDGRVCLLGHLDATVEVKGEAGEVTLAFCFHGPALDQAIAEL
HHHHHHHHHCCCCEEEECCCCCEEEEEECCEEEEEECCCCCEEEEEEECCHHHHHHHHHH
GATPLPPYIASKRAPDDRDAADYQTMFAANEGAVAAPTAGLHFTPELDAALAARGVTVHR
CCCCCCCHHHCCCCCCCCCCHHHHHEEECCCCCEECCCCCCEECCCHHHHHHHCCCEEEE
VTLHVGAGTFLPVKTDDTAEHRMHAEWGTIGRDTAAALNAARAQGGRIVAVGTTSLRLLE
EEEEECCCEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHH
SAARDDGTIAPFADETAIFITPGYKFRAVDVLMTNFHLPRSTLFMLVSAFCGLETMQAAY
HHCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHH
RHAIETGYRFYSYGDASLLFREPT
HHHHHHCCEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA