Click here to switch to the map view.

The map label for this gene is pdhA [H]

Identifier: 86749888

GI number: 86749888

Start: 3159282

End: 3160316

Strand: Reverse

Name: pdhA [H]

Synonym: RPB_2771

Alternate gene names: 86749888

Gene position: 3160316-3159282 (Counterclockwise)

Preceding gene: 86749889

Following gene: 86749887

Centisome position: 59.27

GC content: 64.54

Gene sequence:

>1035_bases
ATGGCCGCACCCAAGAAGAGCGCCGCGAAAGAGGCGGGGCAGGACAAGGACCCTGCACCGAACAAGCCGCGGGTCCCGGA
TTTCTCGAAGGAACAGGAGCTGCGCGCGTTTCGCGACATGCTGCTGATCCGCCGCTTCGAAGAGAAAGCCGGCCAGCTCT
ACGGCATGGGTGCGATCGGCGGATTCTGCCATCTCTATATCGGCCAGGAAGCCGTCGTCGTCGGCATGCAGATGGCGCTG
CGCGAGGGCGATCAGGTCATCACTGGCTATCGCGACCACGGCCACATGCTCGCCTGCGACATGGACGCCAAGGGCGTGAT
GGCCGAGCTGACCGGCCGCCGCGGGGGCTACTCCAAGGGCAAGGGCGGCTCCATGCATATGTTCAGCATGGAGAAGCACT
TCTACGGCGGCCACGGCATCGTCGGCGCGCAGGTCTCGCTCGGCACCGGCATCGCCTTCGCCAACCGCTATCGCGACAAT
GGCAGCGTCTGCCTGGCCTATTTCGGCGACGGCGCCTCCAATCAGGGGCAGGTCTACGAGAGCTTCAACATGGCGGAGCT
GTGGAAGCTCCCCGTGGTCTACGTCATCGAGAACAACCGCTACGCGATGGGCACGTCGGTGACGCGTTCGTCGGCGCAGA
CCGACTTCTCCAAGCGCGGCATCTCGTTCAACATTCCCGGCGAGCAGGTCGACGGCATGGACGTCCGCGCGGTCAAGGCC
GCGGGCGACAAGGCGGTGGCGCATTGCCGCGCCGGCAACGGCCCCTACATCCTGGAGATGCAGACCTATCGCTATCGTGG
CCACTCGATGTCGGACCCGGCGAAGTACCGGACCCGCGAGGAGGTCGACAAGATCCGCAACGATCAGGACCCGATCGAGC
AGGTGCGGCAGCGTCTGCTCGGGCAGGACATGACCGAGGACGATCTGAAGAAGATCGACGCCGAGATCCGCAAGATCGTC
AACGAGGCGGCCGATTTCGCGCAGAACGATCCCGAGCCAGATCCCGCCGAACTCTACACCGATGTGTATCGCTGA

Upstream 100 bases:

>100_bases
GCAGTGCGGCAAACTGAATTGCGTGTTGCGGCGCGCGGCTCCTTTCACGACTGAATAAGTTACGGTAGAGAGGCTGATCT
TCTCTCTCATCGGAATCGCC

Downstream 100 bases:

>100_bases
TGATGTCCGTCGCTGACGCCGATCGCGCGAATGCGCCTGTGCCGCTGTCGCGCGGCGATCGCGCGCATGATTGAATGACT
GCGGAGCCCAAATGCCAACT

Product: pyruvate dehydrogenase alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAAPKKSAAKEAGQDKDPAPNKPRVPDFSKEQELRAFRDMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAVVVGMQMAL
REGDQVITGYRDHGHMLACDMDAKGVMAELTGRRGGYSKGKGGSMHMFSMEKHFYGGHGIVGAQVSLGTGIAFANRYRDN
GSVCLAYFGDGASNQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGISFNIPGEQVDGMDVRAVKA
AGDKAVAHCRAGNGPYILEMQTYRYRGHSMSDPAKYRTREEVDKIRNDQDPIEQVRQRLLGQDMTEDDLKKIDAEIRKIV
NEAADFAQNDPEPDPAELYTDVYR

Sequences:

>Translated_344_residues
MAAPKKSAAKEAGQDKDPAPNKPRVPDFSKEQELRAFRDMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAVVVGMQMAL
REGDQVITGYRDHGHMLACDMDAKGVMAELTGRRGGYSKGKGGSMHMFSMEKHFYGGHGIVGAQVSLGTGIAFANRYRDN
GSVCLAYFGDGASNQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGISFNIPGEQVDGMDVRAVKA
AGDKAVAHCRAGNGPYILEMQTYRYRGHSMSDPAKYRTREEVDKIRNDQDPIEQVRQRLLGQDMTEDDLKKIDAEIRKIV
NEAADFAQNDPEPDPAELYTDVYR
>Mature_343_residues
AAPKKSAAKEAGQDKDPAPNKPRVPDFSKEQELRAFRDMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAVVVGMQMALR
EGDQVITGYRDHGHMLACDMDAKGVMAELTGRRGGYSKGKGGSMHMFSMEKHFYGGHGIVGAQVSLGTGIAFANRYRDNG
SVCLAYFGDGASNQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGISFNIPGEQVDGMDVRAVKAA
GDKAVAHCRAGNGPYILEMQTYRYRGHSMSDPAKYRTREEVDKIRNDQDPIEQVRQRLLGQDMTEDDLKKIDAEIRKIVN
EAADFAQNDPEPDPAELYTDVYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=323, Percent_Identity=51.3931888544892, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI291084742, Length=323, Percent_Identity=51.0835913312694, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI4505685, Length=323, Percent_Identity=51.0835913312694, Blast_Score=314, Evalue=7e-86,
Organism=Homo sapiens, GI291084744, Length=330, Percent_Identity=50, Blast_Score=307, Evalue=7e-84,
Organism=Homo sapiens, GI291084757, Length=323, Percent_Identity=45.8204334365325, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI11386135, Length=321, Percent_Identity=29.9065420560748, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI258645172, Length=321, Percent_Identity=30.5295950155763, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32564172, Length=329, Percent_Identity=50.4559270516717, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17536047, Length=329, Percent_Identity=50.4559270516717, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI86563355, Length=326, Percent_Identity=25.7668711656442, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI86563357, Length=326, Percent_Identity=25.7668711656442, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321026, Length=326, Percent_Identity=48.4662576687117, Blast_Score=303, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24639744, Length=318, Percent_Identity=50.6289308176101, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI28571106, Length=318, Percent_Identity=50.6289308176101, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI24639740, Length=318, Percent_Identity=50.6289308176101, Blast_Score=323, Evalue=9e-89,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=51.6339869281046, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24639748, Length=319, Percent_Identity=50.4702194357367, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI21355903, Length=322, Percent_Identity=25.776397515528, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 38167; Mature: 38035

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPKKSAAKEAGQDKDPAPNKPRVPDFSKEQELRAFRDMLLIRRFEEKAGQLYGMGAIG
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCHHC
GFCHLYIGQEAVVVGMQMALREGDQVITGYRDHGHMLACDMDAKGVMAELTGRRGGYSKG
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHCCCCCCCCCC
KGGSMHMFSMEKHFYGGHGIVGAQVSLGTGIAFANRYRDNGSVCLAYFGDGASNQGQVYE
CCCCEEEEEHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCCCEEC
SFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGISFNIPGEQVDGMDVRAVKA
CCCHHHHHCCCEEEEEECCCEEECCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEC
AGDKAVAHCRAGNGPYILEMQTYRYRGHSMSDPAKYRTREEVDKIRNDQDPIEQVRQRLL
CCCHHHEEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
GQDMTEDDLKKIDAEIRKIVNEAADFAQNDPEPDPAELYTDVYR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AAPKKSAAKEAGQDKDPAPNKPRVPDFSKEQELRAFRDMLLIRRFEEKAGQLYGMGAIG
CCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCHHC
GFCHLYIGQEAVVVGMQMALREGDQVITGYRDHGHMLACDMDAKGVMAELTGRRGGYSKG
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHCCCCCCCCCC
KGGSMHMFSMEKHFYGGHGIVGAQVSLGTGIAFANRYRDNGSVCLAYFGDGASNQGQVYE
CCCCEEEEEHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCCCEEC
SFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGISFNIPGEQVDGMDVRAVKA
CCCHHHHHCCCEEEEEECCCEEECCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEC
AGDKAVAHCRAGNGPYILEMQTYRYRGHSMSDPAKYRTREEVDKIRNDQDPIEQVRQRLL
CCCHHHEEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
GQDMTEDDLKKIDAEIRKIVNEAADFAQNDPEPDPAELYTDVYR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]