Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 86749887

GI number: 86749887

Start: 3157787

End: 3159190

Strand: Reverse

Name: pdhB [H]

Synonym: RPB_2770

Alternate gene names: 86749887

Gene position: 3159190-3157787 (Counterclockwise)

Preceding gene: 86749888

Following gene: 86749886

Centisome position: 59.25

GC content: 65.67

Gene sequence:

>1404_bases
ATGCCAACTCAAGTTCTGATGCCCGCGCTGTCGCCGACCATGGAGAAGGGCAACCTTTCGAAATGGCTCAAGAAGGAAGG
CGACAAGGTCAAGAGCGGCGACGTGATCGCGGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCCGCCGATGACG
GCACGCTCGGCAAGATCCTGATCCCCGAAGGCACCAACGACGTCGCGGTCAACACCCCGATCGCCACTATCCTCGGCGAC
GGCGAAAGCGCCGCCGATGCCGACAAGGCACCCGATCCTGCCGCGCAGAACAAGAGCTCGCAGTCTGCACCGCCGGCCGC
AGCATCGGAAGCCGCTGAACCGAAGTCAGCGCCTGCGCAGAGTGCTCCGGAAGCGCCCGCTGTCTCGGCTGCCGCAGATC
CAGACATTCCCGCCGGCACCGAAATGGTGACGCAGACCATCCGCGAAGCGCTGCGTGACGCGATGGCCGAGGAGATGCGT
CGCGATCCCGACGTGTTCGTGATGGGCGAGGAAGTCGCCGAGTATCAGGGCGCCTACAAGGTGACGCAGGGGCTGCTGCA
GGAGTTCGGCGCGCGCCGCGTGATCGACACCCCGATCACCGAGCACGGCTTCGCCGGCGTCGGCGTCGGCGCGGCGATGA
CCGGGCTGAAGCCGATTGTCGAATTCATGACCTTCAACTTCGCCATGCAGGCGATCGACCAGATCATCAATTCCGCCGCC
AAGACGCTGTACATGTCCGGCGGGCAACTCGGCTGCTCGATCGTCTTCCGCGGCCCCAACGGCGCGGCCTCGCGCGTCGC
CGCGCAGCACAGCCAAGACTACTCGGCCTGGTACGCGCAGATTCCCGGCCTCAAGGTGGTCGCGCCGTCGACCGCCGCCG
ACTACAAGGGCCTGCTCAAGGCAGCGATCCGCGATCCCAACCCGGTGATCTTCCTCGAACACGAGATGATGTACGGGCAG
TCCGGCGAAGTGCCGAAGCTCGACGATTTCGTCATTCCGATCGGCAAGGCGCGCATCGCGCGCGCGGGCGAGCACGTCAC
GCTGATCTCGTGGTCGCACGGCATGACCTACGCGCTGAAGGCGGCCGAGGAACTGGCCAAAGACGGCATCGACGCCGAAG
TGATCGACCTGCGCACGCTGCGGCCGCTCGACACCGAGACGATCATCGCCTCGGTGAAGAAGACCGGCCGCGCGGTGGCG
GTCGAGGAGGGCTGGCAGCAGAACGGCGTCGGCGCCGAACTCGCTGCGCGGATTATGGAGCACGCGTTCGATTATCTGGA
TGCGCCGGTCAAGCGCGTGTCGGGCAAGGACGTGCCGATGCCTTACGCAGCCAATCTCGAGAAGCTGGCGCTGCCGAGCG
TCGCCGAAGTCGTCGAGGCGGCCAAAGCCGTCTGCTATCGGTGA

Upstream 100 bases:

>100_bases
TATCGCTGATGATGTCCGTCGCTGACGCCGATCGCGCGAATGCGCCTGTGCCGCTGTCGCGCGGCGATCGCGCGCATGAT
TGAATGACTGCGGAGCCCAA

Downstream 100 bases:

>100_bases
GGCCATGACCGGCCCGAAGCAACAGCCGCTGCCGCCCGACGTGGTCGGCCGCGAGGACGCCACCGAGGTGCTGCGCGCTT
TCGTGCTCGACGGCGGGCTG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPTQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADDGTLGKILIPEGTNDVAVNTPIATILGD
GESAADADKAPDPAAQNKSSQSAPPAAASEAAEPKSAPAQSAPEAPAVSAAADPDIPAGTEMVTQTIREALRDAMAEEMR
RDPDVFVMGEEVAEYQGAYKVTQGLLQEFGARRVIDTPITEHGFAGVGVGAAMTGLKPIVEFMTFNFAMQAIDQIINSAA
KTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPSTAADYKGLLKAAIRDPNPVIFLEHEMMYGQ
SGEVPKLDDFVIPIGKARIARAGEHVTLISWSHGMTYALKAAEELAKDGIDAEVIDLRTLRPLDTETIIASVKKTGRAVA
VEEGWQQNGVGAELAARIMEHAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR

Sequences:

>Translated_467_residues
MPTQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADDGTLGKILIPEGTNDVAVNTPIATILGD
GESAADADKAPDPAAQNKSSQSAPPAAASEAAEPKSAPAQSAPEAPAVSAAADPDIPAGTEMVTQTIREALRDAMAEEMR
RDPDVFVMGEEVAEYQGAYKVTQGLLQEFGARRVIDTPITEHGFAGVGVGAAMTGLKPIVEFMTFNFAMQAIDQIINSAA
KTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPSTAADYKGLLKAAIRDPNPVIFLEHEMMYGQ
SGEVPKLDDFVIPIGKARIARAGEHVTLISWSHGMTYALKAAEELAKDGIDAEVIDLRTLRPLDTETIIASVKKTGRAVA
VEEGWQQNGVGAELAARIMEHAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR
>Mature_466_residues
PTQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADDGTLGKILIPEGTNDVAVNTPIATILGDG
ESAADADKAPDPAAQNKSSQSAPPAAASEAAEPKSAPAQSAPEAPAVSAAADPDIPAGTEMVTQTIREALRDAMAEEMRR
DPDVFVMGEEVAEYQGAYKVTQGLLQEFGARRVIDTPITEHGFAGVGVGAAMTGLKPIVEFMTFNFAMQAIDQIINSAAK
TLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPSTAADYKGLLKAAIRDPNPVIFLEHEMMYGQS
GEVPKLDDFVIPIGKARIARAGEHVTLISWSHGMTYALKAAEELAKDGIDAEVIDLRTLRPLDTETIIASVKKTGRAVAV
EEGWQQNGVGAELAARIMEHAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=321, Percent_Identity=59.190031152648, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI291084858, Length=321, Percent_Identity=56.0747663551402, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=35.6037151702786, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=35.6037151702786, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=51.8518518518518, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=51.8518518518518, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI31711992, Length=132, Percent_Identity=42.4242424242424, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI260898739, Length=81, Percent_Identity=50.6172839506173, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI225637463, Length=322, Percent_Identity=27.3291925465839, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI225637461, Length=322, Percent_Identity=27.3291925465839, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI225637459, Length=322, Percent_Identity=27.3291925465839, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI133778974, Length=241, Percent_Identity=29.4605809128631, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=62.5386996904025, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17506935, Length=333, Percent_Identity=37.8378378378378, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17560088, Length=134, Percent_Identity=44.7761194029851, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17539652, Length=257, Percent_Identity=30.7392996108949, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=59.0214067278288, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324258, Length=136, Percent_Identity=50, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321632, Length=71, Percent_Identity=57.7464788732394, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=61.0591900311526, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=61.0591900311526, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI160714832, Length=315, Percent_Identity=39.0476190476191, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI160714828, Length=315, Percent_Identity=39.0476190476191, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=62.9213483146067, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=62.9213483146067, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=82, Percent_Identity=53.6585365853659, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45551847, Length=254, Percent_Identity=31.496062992126, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45550715, Length=254, Percent_Identity=31.496062992126, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645119, Length=254, Percent_Identity=31.496062992126, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24582497, Length=72, Percent_Identity=51.3888888888889, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24666278, Length=262, Percent_Identity=28.6259541984733, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49431; Mature: 49299

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADDGTLGKIL
CCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEEEE
IPEGTNDVAVNTPIATILGDGESAADADKAPDPAAQNKSSQSAPPAAASEAAEPKSAPAQ
ECCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
SAPEAPAVSAAADPDIPAGTEMVTQTIREALRDAMAEEMRRDPDVFVMGEEVAEYQGAYK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHH
VTQGLLQEFGARRVIDTPITEHGFAGVGVGAAMTGLKPIVEFMTFNFAMQAIDQIINSAA
HHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPSTAADYKGLLK
HHEEECCCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCEEEECCCCHHHHHHHHH
AAIRDPNPVIFLEHEMMYGQSGEVPKLDDFVIPIGKARIARAGEHVTLISWSHGMTYALK
HHHCCCCCEEEEEEHHHCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEECCCCHHHHH
AAEELAKDGIDAEVIDLRTLRPLDTETIIASVKKTGRAVAVEEGWQQNGVGAELAARIME
HHHHHHHCCCCEEEEEHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
HAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR
HHHHHHHHHHHHCCCCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADDGTLGKIL
CCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEEEE
IPEGTNDVAVNTPIATILGDGESAADADKAPDPAAQNKSSQSAPPAAASEAAEPKSAPAQ
ECCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
SAPEAPAVSAAADPDIPAGTEMVTQTIREALRDAMAEEMRRDPDVFVMGEEVAEYQGAYK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHH
VTQGLLQEFGARRVIDTPITEHGFAGVGVGAAMTGLKPIVEFMTFNFAMQAIDQIINSAA
HHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPSTAADYKGLLK
HHEEECCCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCEEEECCCCHHHHHHHHH
AAIRDPNPVIFLEHEMMYGQSGEVPKLDDFVIPIGKARIARAGEHVTLISWSHGMTYALK
HHHCCCCCEEEEEEHHHCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEECCCCHHHHH
AAEELAKDGIDAEVIDLRTLRPLDTETIIASVKKTGRAVAVEEGWQQNGVGAELAARIME
HHHHHHHCCCCEEEEEHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
HAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR
HHHHHHHHHHHHCCCCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]