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The map label for this gene is dhfrIII [H]

Identifier: 86749159

GI number: 86749159

Start: 2316856

End: 2317395

Strand: Direct

Name: dhfrIII [H]

Synonym: RPB_2037

Alternate gene names: 86749159

Gene position: 2316856-2317395 (Clockwise)

Preceding gene: 86749158

Following gene: 86749160

Centisome position: 43.45

GC content: 63.7

Gene sequence:

>540_bases
GTGAGTAGCCCGGGAGTTAGCTCGCGACTGTCGATCGTCCTCATCGTGGCGGTTGCCGACAATGGCGTGATCGGGCGGGA
CAACGCGATTCCGTGGCGGCTGAAATCCGACCTGCAGCGCTTCAAGCAACTGACGCTGAACAAGCCGATCATCATGGGTC
GCAAGACCTATCAGTCGATCGGCCGGCCGTTGCCGGGACGAACCAATATCGTGGTGACCCGAGACCCGACCTTCCATGCG
GACGGCGTGGTGGTCGTGCCGTCATTCGACGCCGCGGAGCAAGTCGCATGCGGCGATGCGATGCGGCGTTTTGCCACCGA
AATCGCGGTGATCGGCGGCGCGGAAATCTATGCGCACTGGCTGCCGCGCGCGACTCGGATCGAATTGACCGAGGTACATG
CGAGCCCGCCGGGCGATGCGACTTTTCCCGCGCTCGACCCGTCGCAGTGGGAGGAAACGGCTCGGACGCATCACGCAGCC
GGCCCCCAGGACAGCGCCGATGTCTCGTATGTGACCTATCGACGCTGCACTGCACGTTAA

Upstream 100 bases:

>100_bases
CGATCAATCCCGCCGTGACCGACATCTTCGGCTTCCGCTACGAAGACTTCACCCTGCACGGCTACGATCCGCACCCGCAC
ATCAAGGCGGAGGTGGCGGT

Downstream 100 bases:

>100_bases
CCTTGAGCACCGCGCGATCCACATTGACAAAATTGCGGCAGGGCTTAGCTCGCGACAAGTGACGACGCGCGTTGTGCCTG
CCGGGCCCGTCCCCTATAAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSSPGVSSRLSIVLIVAVADNGVIGRDNAIPWRLKSDLQRFKQLTLNKPIIMGRKTYQSIGRPLPGRTNIVVTRDPTFHA
DGVVVVPSFDAAEQVACGDAMRRFATEIAVIGGAEIYAHWLPRATRIELTEVHASPPGDATFPALDPSQWEETARTHHAA
GPQDSADVSYVTYRRCTAR

Sequences:

>Translated_179_residues
MSSPGVSSRLSIVLIVAVADNGVIGRDNAIPWRLKSDLQRFKQLTLNKPIIMGRKTYQSIGRPLPGRTNIVVTRDPTFHA
DGVVVVPSFDAAEQVACGDAMRRFATEIAVIGGAEIYAHWLPRATRIELTEVHASPPGDATFPALDPSQWEETARTHHAA
GPQDSADVSYVTYRRCTAR
>Mature_178_residues
SSPGVSSRLSIVLIVAVADNGVIGRDNAIPWRLKSDLQRFKQLTLNKPIIMGRKTYQSIGRPLPGRTNIVVTRDPTFHAD
GVVVVPSFDAAEQVACGDAMRRFATEIAVIGGAEIYAHWLPRATRIELTEVHASPPGDATFPALDPSQWEETARTHHAAG
PQDSADVSYVTYRRCTAR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=164, Percent_Identity=45.1219512195122, Blast_Score=133, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=176, Percent_Identity=31.8181818181818, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=175, Percent_Identity=32.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=175, Percent_Identity=29.7142857142857, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647458, Length=175, Percent_Identity=29.7142857142857, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19532; Mature: 19401

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPGVSSRLSIVLIVAVADNGVIGRDNAIPWRLKSDLQRFKQLTLNKPIIMGRKTYQSI
CCCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHH
GRPLPGRTNIVVTRDPTFHADGVVVVPSFDAAEQVACGDAMRRFATEIAVIGGAEIYAHW
CCCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHH
LPRATRIELTEVHASPPGDATFPALDPSQWEETARTHHAAGPQDSADVSYVTYRRCTAR
CCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SSPGVSSRLSIVLIVAVADNGVIGRDNAIPWRLKSDLQRFKQLTLNKPIIMGRKTYQSI
CCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHH
GRPLPGRTNIVVTRDPTFHADGVVVVPSFDAAEQVACGDAMRRFATEIAVIGGAEIYAHW
CCCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHH
LPRATRIELTEVHASPPGDATFPALDPSQWEETARTHHAAGPQDSADVSYVTYRRCTAR
CCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]