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The map label for this gene is thyA

Identifier: 86749158

GI number: 86749158

Start: 2316065

End: 2316859

Strand: Direct

Name: thyA

Synonym: RPB_2036

Alternate gene names: 86749158

Gene position: 2316065-2316859 (Clockwise)

Preceding gene: 86749157

Following gene: 86749159

Centisome position: 43.44

GC content: 63.4

Gene sequence:

>795_bases
ATGATTCCGTACCACGACCTGCTCGAACGCATCCTGAGCGACGGCGCCGAAAAACACGACCGCACCGGCACCGGCACGCT
GTCGGTGTTCGGCCATCAGCTTCGGTTCGATCTCGCCGCCGGTTTTCCGATGCTGACCACCAAGCGGTTACCGCTCAAGG
CGATCATTCACGAGCTGCTGTGGTTTCTGCAGGGCGACACCAACATCAAATATCTGCACGACCACGGCGTCACGATCTGG
GACGAATGGGCGGACGCCAACGGTGACCTCGGCCCTGTCTATGGCGCGCAATGGCGCTCGTGGCCGACCGCGGACGGCCG
CAGCATCGATCAGATCGCCCATGTCGTCGACATGATCAGGCGCAACCCGGACTCGCGTCGGCTGATCGTCACGGCGTGGA
ATCCGGCGGATGTCGAGAAGATGGCGTTGCCGCCGTGTCATTGCCTGTTCCAGTTCTACGTCGCGAACGGCAGGCTATCG
TGCCAATTGTATCAGCGCTCGGCGGACGTGTTTCTCGGCGTGCCGTTCAACATCGCGTCCTACGCGCTGCTGACGATGAT
GGTGGCGCAGGTCACGGGGCTGAAGCCCGGCGACTTCATCCACACGCTCGGCGACGCGCATCTGTATTCCAATCATCTGG
AGCAGGCGCGGCTGCAACTGACCCGCGCCCCGCGCGCGCTGCCGGCGATGGCGATCAATCCCGCCGTGACCGACATCTTC
GGCTTCCGCTACGAAGACTTCACCCTGCACGGCTACGATCCGCACCCGCACATCAAGGCGGAGGTGGCGGTGTGA

Upstream 100 bases:

>100_bases
GCAGGGGTATTTTCGGCATGAAATCCGAAGATCCGCAGCCTCGCGCAGCGACCGCTCCCGGCGGACGCCACGCCGGCGCC
ATTCCGGACACCGCCGACCG

Downstream 100 bases:

>100_bases
GTAGCCCGGGAGTTAGCTCGCGACTGTCGATCGTCCTCATCGTGGCGGTTGCCGACAATGGCGTGATCGGGCGGGACAAC
GCGATTCCGTGGCGGCTGAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIPYHDLLERILSDGAEKHDRTGTGTLSVFGHQLRFDLAAGFPMLTTKRLPLKAIIHELLWFLQGDTNIKYLHDHGVTIW
DEWADANGDLGPVYGAQWRSWPTADGRSIDQIAHVVDMIRRNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGRLS
CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKPGDFIHTLGDAHLYSNHLEQARLQLTRAPRALPAMAINPAVTDIF
GFRYEDFTLHGYDPHPHIKAEVAV

Sequences:

>Translated_264_residues
MIPYHDLLERILSDGAEKHDRTGTGTLSVFGHQLRFDLAAGFPMLTTKRLPLKAIIHELLWFLQGDTNIKYLHDHGVTIW
DEWADANGDLGPVYGAQWRSWPTADGRSIDQIAHVVDMIRRNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGRLS
CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKPGDFIHTLGDAHLYSNHLEQARLQLTRAPRALPAMAINPAVTDIF
GFRYEDFTLHGYDPHPHIKAEVAV
>Mature_264_residues
MIPYHDLLERILSDGAEKHDRTGTGTLSVFGHQLRFDLAAGFPMLTTKRLPLKAIIHELLWFLQGDTNIKYLHDHGVTIW
DEWADANGDLGPVYGAQWRSWPTADGRSIDQIAHVVDMIRRNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGRLS
CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKPGDFIHTLGDAHLYSNHLEQARLQLTRAPRALPAMAINPAVTDIF
GFRYEDFTLHGYDPHPHIKAEVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=54.8042704626335, Blast_Score=305, Evalue=2e-83,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=64.7727272727273, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=47.8723404255319, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=227, Percent_Identity=48.0176211453744, Blast_Score=213, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=55.4770318021201, Blast_Score=311, Evalue=3e-85,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_RHOP2 (Q2IYG7)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485654.1
- HSSP:   P0A884
- ProteinModelPortal:   Q2IYG7
- SMR:   Q2IYG7
- STRING:   Q2IYG7
- GeneID:   3909851
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2036
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   RPAL316058:RPB_2036-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 29751; Mature: 29751

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPYHDLLERILSDGAEKHDRTGTGTLSVFGHQLRFDLAAGFPMLTTKRLPLKAIIHELL
CCCHHHHHHHHHHCCHHHCCCCCCCCCEEHHHHHEEHHHCCCCCEECCCCCHHHHHHHHH
WFLQGDTNIKYLHDHGVTIWDEWADANGDLGPVYGAQWRSWPTADGRSIDQIAHVVDMIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGRLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVTGLKPGDFIHTLGDAHLYSNHLEQARLQLTRAPRALPAMAINPAVTDIF
HHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHH
GFRYEDFTLHGYDPHPHIKAEVAV
CCCCCCEEEECCCCCCCCEEEECC
>Mature Secondary Structure
MIPYHDLLERILSDGAEKHDRTGTGTLSVFGHQLRFDLAAGFPMLTTKRLPLKAIIHELL
CCCHHHHHHHHHHCCHHHCCCCCCCCCEEHHHHHEEHHHCCCCCEECCCCCHHHHHHHHH
WFLQGDTNIKYLHDHGVTIWDEWADANGDLGPVYGAQWRSWPTADGRSIDQIAHVVDMIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGRLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVTGLKPGDFIHTLGDAHLYSNHLEQARLQLTRAPRALPAMAINPAVTDIF
HHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHH
GFRYEDFTLHGYDPHPHIKAEVAV
CCCCCCEEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA