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The map label for this gene is fixJ [H]

Identifier: 86748488

GI number: 86748488

Start: 1553317

End: 1553934

Strand: Reverse

Name: fixJ [H]

Synonym: RPB_1363

Alternate gene names: 86748488

Gene position: 1553934-1553317 (Counterclockwise)

Preceding gene: 86748489

Following gene: 86748487

Centisome position: 29.15

GC content: 62.46

Gene sequence:

>618_bases
ATGCCACGTAGGGGGAACGTGTACGTCATCGACGACGATCAGGCGATGCGGGACTCGCTCGACTTCCTGCTCGGATCTGC
CGATTTCGAGGTGAGGCTGTTCGAGTCCGCGCTGGATTTCCTCAACGAGGTGACGATGCTGCCGTTCGGCTGCATCGTCT
CCGACGTGCGGATGCCCGGCATGAACGGCATGGACCTGCTGAAGCAGCTCAAGAACGACCGCACGCGGCTTCCGGTGGTG
ATCATGACCGGGCATGGCGACGTTCCGCTGGCGGTCGAGGCGATGAAGCTCGGCGCGATCGACTTCATCGAGAAGCCGTT
CGAGGACGACCGGCTGATCGGAATGATCGAAACCGCGCTGAAGCAGGCCGGCGACGAAGCCAAAAGCGAGGCGGTGTCGC
AGGATATCGTCGCGCGGATCCAGTCGCTGAGCCCGCGCGAACGCCAAGTGATGGACGGTCTGATCGCCGGCATGTCGAAC
AAGGTGATCGCCCGTGAGTTCGATATCAGCCCGCGGACGATCGAGGTCTATCGCGCCAACGTCATGACCAAGATGCAGGC
CGGCAGCTTGTCGGAGCTGGTTCGGATGGCGATGCGTGGCGGCGTGATCAGGGATTGA

Upstream 100 bases:

>100_bases
ATCGAGGCACATGGCGGCCGGATGTGGGCCGAATCAAACCCCTCGGGGGGCGCGACCTTCCGCTTCACGTTGCCTGCCGC
GGCCCAAGAGGACCTCGCCG

Downstream 100 bases:

>100_bases
GGCAGATCAACCTGCCTCGCACGCCAGCAACTATTCTCCCCTGCATGAAAGAATTGCAGTCAGGTCGGGTGCGTGGGACG
AACTGATGACCACCGCATCC

Product: response regulator FixJ

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MPRRGNVYVIDDDQAMRDSLDFLLGSADFEVRLFESALDFLNEVTMLPFGCIVSDVRMPGMNGMDLLKQLKNDRTRLPVV
IMTGHGDVPLAVEAMKLGAIDFIEKPFEDDRLIGMIETALKQAGDEAKSEAVSQDIVARIQSLSPRERQVMDGLIAGMSN
KVIAREFDISPRTIEVYRANVMTKMQAGSLSELVRMAMRGGVIRD

Sequences:

>Translated_205_residues
MPRRGNVYVIDDDQAMRDSLDFLLGSADFEVRLFESALDFLNEVTMLPFGCIVSDVRMPGMNGMDLLKQLKNDRTRLPVV
IMTGHGDVPLAVEAMKLGAIDFIEKPFEDDRLIGMIETALKQAGDEAKSEAVSQDIVARIQSLSPRERQVMDGLIAGMSN
KVIAREFDISPRTIEVYRANVMTKMQAGSLSELVRMAMRGGVIRD
>Mature_204_residues
PRRGNVYVIDDDQAMRDSLDFLLGSADFEVRLFESALDFLNEVTMLPFGCIVSDVRMPGMNGMDLLKQLKNDRTRLPVVI
MTGHGDVPLAVEAMKLGAIDFIEKPFEDDRLIGMIETALKQAGDEAKSEAVSQDIVARIQSLSPRERQVMDGLIAGMSNK
VIAREFDISPRTIEVYRANVMTKMQAGSLSELVRMAMRGGVIRD

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=130, Percent_Identity=36.9230769230769, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790437, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=31.304347826087, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788905, Length=131, Percent_Identity=32.824427480916, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788222, Length=159, Percent_Identity=25.1572327044025, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789402, Length=199, Percent_Identity=25.6281407035176, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789809, Length=132, Percent_Identity=25.7575757575758, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=159, Percent_Identity=30.8176100628931, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1790860, Length=120, Percent_Identity=26.6666666666667, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22798; Mature: 22667

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRGNVYVIDDDQAMRDSLDFLLGSADFEVRLFESALDFLNEVTMLPFGCIVSDVRMPG
CCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MNGMDLLKQLKNDRTRLPVVIMTGHGDVPLAVEAMKLGAIDFIEKPFEDDRLIGMIETAL
CCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHH
KQAGDEAKSEAVSQDIVARIQSLSPRERQVMDGLIAGMSNKVIAREFDISPRTIEVYRAN
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHH
VMTKMQAGSLSELVRMAMRGGVIRD
HHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PRRGNVYVIDDDQAMRDSLDFLLGSADFEVRLFESALDFLNEVTMLPFGCIVSDVRMPG
CCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MNGMDLLKQLKNDRTRLPVVIMTGHGDVPLAVEAMKLGAIDFIEKPFEDDRLIGMIETAL
CCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHH
KQAGDEAKSEAVSQDIVARIQSLSPRERQVMDGLIAGMSNKVIAREFDISPRTIEVYRAN
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHH
VMTKMQAGSLSELVRMAMRGGVIRD
HHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]