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The map label for this gene is fixL [H]

Identifier: 86748489

GI number: 86748489

Start: 1553924

End: 1555456

Strand: Reverse

Name: fixL [H]

Synonym: RPB_1364

Alternate gene names: 86748489

Gene position: 1555456-1553924 (Counterclockwise)

Preceding gene: 86748490

Following gene: 86748488

Centisome position: 29.17

GC content: 62.23

Gene sequence:

>1533_bases
GTGACGGATCAAGCCCCATCGTCATCGGAAGCCCTGGCGCGCGATCGGCGGCTGCAGCAGGGAATTGATCAATACGGCGT
GGGAAGTTGGGAACTCAATCTCGCCACGCGGGAATTGGCGTGGTCTCCGACGACCCGGCGGCTGTACGGCGTCACCACCG
ATACGCCGATCGATTTCGATGCCTTCATCGCGCTGGTCGATCTGGAAGACCGCGAGCGCGTCGCGCAGGCGCTGCAACGG
TCGATCGATCGCGGCGAGCATCTCGACATCATGTACCGGGTCGCAGATCGTGGTCAGCCCAGGCACTGGTTGCGCGCCCG
TGGCGGCCTGATCAAAGTCGAAGGCACGGCCGGGTATCTCTGTGGCATCGTTCTCGACATCGATCAGCAGAAGGTGCTCG
AGCAGGAACTCCGACTGCAGCAAAGCCAGTTGCGCTCGATTCTGGACACCGTGCCCGACGCGATGATCCTCATCGACGGC
CGGGGTATCATGCGGTTCTTCTCCAGCGCGGCCGAAAGGATGTTCGGGCTGTCCGAGAAAGAGGCGATCGGCCAGAACAT
CAGCGTTCTGATGCCCCGCCCGGACGCATCCCGGCACGACAACTACATTTCCCGCTACAACTCCACCGGAGAGCGCCACA
TCATCGGCATCGGCCGCATCGTCACCGGTCAGCGGAAGGACGGAACCACCTTCCCGATCCATCTGTCGATCGGCGAAATG
GAATCCGGCGGTGAGCGATATTTCACCGGCTTCATCCGCGATCTCACCGAGTACCAGCAGACTCAGGCGCGCCTCCACGA
ATTGCAGGCCGAACTGGTCCACGTCTCGCGGCTCACCGCGATGGGCGAAATGGCGTCGGCTCTGGCGCACGAGCTGAACC
AGCCGCTGTCCGCGATCAGCAATTACATGAAAGGTTCGCGCCGGCTGCTCGCCGACAGCAGCGATCCGAACGTGCCCAAG
ATCGAACACGCGATGGAACGGGCGGCCGAGCAGGCGCTGCGCGCCGGACAGATCATCCGCCGATTGCGTGATTTCGTCGC
CCGCGGCGAATCCGAAAAACGGGTCGAAAGCCTCGCCAAGCTGGTCGAGGAGGCCGGTGCCTTGGGCCTGACCGGCGCCC
GCGAACAGGGCGTGGTGCTGCGCTTCAAGCTCGATCCGGCGAGCGACATGGCGCTGGTCGACCGGGTTCAGATCCAGCAA
GTGCTCGTCAATTTGTTTCGCAACGCTCTTGAAGCGATGGCCAATTCGGAGAAAAAGGAACTGGTCGTCTCCAACGCCCG
CGTTGCCGATCAAATGATTGAGATTTCCGTCGCCGATACCGGCAGCGGGTTCCACGAGGACGTGAAATCAAGTCTGTTTC
AGACCTTCTTCACCACCAAAGAAACCGGAATGGGTGTTGGATTGTCGATAAGCCGCTCGATCATCGAGGCACATGGCGGC
CGGATGTGGGCCGAATCAAACCCCTCGGGGGGCGCGACCTTCCGCTTCACGTTGCCTGCCGCGGCCCAAGAGGACCTCGC
CGATGCCACGTAG

Upstream 100 bases:

>100_bases
CGGACGGTTTGCTGCTTCGAATTGCGTTTGTGCACTGCGTCCGTTGCGGACCAGAGCACCGATATGATAAGAGCAATTCA
ATGAGTTGCGAAGGTATCGC

Downstream 100 bases:

>100_bases
GGGGAACGTGTACGTCATCGACGACGATCAGGCGATGCGGGACTCGCTCGACTTCCTGCTCGGATCTGCCGATTTCGAGG
TGAGGCTGTTCGAGTCCGCG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTDQAPSSSEALARDRRLQQGIDQYGVGSWELNLATRELAWSPTTRRLYGVTTDTPIDFDAFIALVDLEDRERVAQALQR
SIDRGEHLDIMYRVADRGQPRHWLRARGGLIKVEGTAGYLCGIVLDIDQQKVLEQELRLQQSQLRSILDTVPDAMILIDG
RGIMRFFSSAAERMFGLSEKEAIGQNISVLMPRPDASRHDNYISRYNSTGERHIIGIGRIVTGQRKDGTTFPIHLSIGEM
ESGGERYFTGFIRDLTEYQQTQARLHELQAELVHVSRLTAMGEMASALAHELNQPLSAISNYMKGSRRLLADSSDPNVPK
IEHAMERAAEQALRAGQIIRRLRDFVARGESEKRVESLAKLVEEAGALGLTGAREQGVVLRFKLDPASDMALVDRVQIQQ
VLVNLFRNALEAMANSEKKELVVSNARVADQMIEISVADTGSGFHEDVKSSLFQTFFTTKETGMGVGLSISRSIIEAHGG
RMWAESNPSGGATFRFTLPAAAQEDLADAT

Sequences:

>Translated_510_residues
MTDQAPSSSEALARDRRLQQGIDQYGVGSWELNLATRELAWSPTTRRLYGVTTDTPIDFDAFIALVDLEDRERVAQALQR
SIDRGEHLDIMYRVADRGQPRHWLRARGGLIKVEGTAGYLCGIVLDIDQQKVLEQELRLQQSQLRSILDTVPDAMILIDG
RGIMRFFSSAAERMFGLSEKEAIGQNISVLMPRPDASRHDNYISRYNSTGERHIIGIGRIVTGQRKDGTTFPIHLSIGEM
ESGGERYFTGFIRDLTEYQQTQARLHELQAELVHVSRLTAMGEMASALAHELNQPLSAISNYMKGSRRLLADSSDPNVPK
IEHAMERAAEQALRAGQIIRRLRDFVARGESEKRVESLAKLVEEAGALGLTGAREQGVVLRFKLDPASDMALVDRVQIQQ
VLVNLFRNALEAMANSEKKELVVSNARVADQMIEISVADTGSGFHEDVKSSLFQTFFTTKETGMGVGLSISRSIIEAHGG
RMWAESNPSGGATFRFTLPAAAQEDLADAT
>Mature_509_residues
TDQAPSSSEALARDRRLQQGIDQYGVGSWELNLATRELAWSPTTRRLYGVTTDTPIDFDAFIALVDLEDRERVAQALQRS
IDRGEHLDIMYRVADRGQPRHWLRARGGLIKVEGTAGYLCGIVLDIDQQKVLEQELRLQQSQLRSILDTVPDAMILIDGR
GIMRFFSSAAERMFGLSEKEAIGQNISVLMPRPDASRHDNYISRYNSTGERHIIGIGRIVTGQRKDGTTFPIHLSIGEME
SGGERYFTGFIRDLTEYQQTQARLHELQAELVHVSRLTAMGEMASALAHELNQPLSAISNYMKGSRRLLADSSDPNVPKI
EHAMERAAEQALRAGQIIRRLRDFVARGESEKRVESLAKLVEEAGALGLTGAREQGVVLRFKLDPASDMALVDRVQIQQV
LVNLFRNALEAMANSEKKELVVSNARVADQMIEISVADTGSGFHEDVKSSLFQTFFTTKETGMGVGLSISRSIIEAHGGR
MWAESNPSGGATFRFTLPAAAQEDLADAT

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=377, Percent_Identity=29.973474801061, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=28.8793103448276, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790300, Length=378, Percent_Identity=26.984126984127, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=403, Percent_Identity=24.317617866005, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=27.5109170305677, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788713, Length=411, Percent_Identity=21.654501216545, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=119, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788381, Length=235, Percent_Identity=22.9787234042553, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87081816, Length=258, Percent_Identity=28.2945736434109, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788393, Length=154, Percent_Identity=29.8701298701299, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081921, Length=122, Percent_Identity=26.2295081967213, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56556; Mature: 56425

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQAPSSSEALARDRRLQQGIDQYGVGSWELNLATRELAWSPTTRRLYGVTTDTPIDFD
CCCCCCCCHHHHHHHHHHHHCHHHHCCCCEEEEEEHHHHCCCCCCCEEEECCCCCCCCHH
AFIALVDLEDRERVAQALQRSIDRGEHLDIMYRVADRGQPRHWLRARGGLIKVEGTAGYL
HEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCEEEEECCCCEE
CGIVLDIDQQKVLEQELRLQQSQLRSILDTVPDAMILIDGRGIMRFFSSAAERMFGLSEK
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHH
EAIGQNISVLMPRPDASRHDNYISRYNSTGERHIIGIGRIVTGQRKDGTTFPIHLSIGEM
HHHCCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEEEECCC
ESGGERYFTGFIRDLTEYQQTQARLHELQAELVHVSRLTAMGEMASALAHELNQPLSAIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NYMKGSRRLLADSSDPNVPKIEHAMERAAEQALRAGQIIRRLRDFVARGESEKRVESLAK
HHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LVEEAGALGLTGAREQGVVLRFKLDPASDMALVDRVQIQQVLVNLFRNALEAMANSEKKE
HHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LVVSNARVADQMIEISVADTGSGFHEDVKSSLFQTFFTTKETGMGVGLSISRSIIEAHGG
HHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
RMWAESNPSGGATFRFTLPAAAQEDLADAT
EEEECCCCCCCEEEEEECCCCCHHHHCCCC
>Mature Secondary Structure 
TDQAPSSSEALARDRRLQQGIDQYGVGSWELNLATRELAWSPTTRRLYGVTTDTPIDFD
CCCCCCCHHHHHHHHHHHHCHHHHCCCCEEEEEEHHHHCCCCCCCEEEECCCCCCCCHH
AFIALVDLEDRERVAQALQRSIDRGEHLDIMYRVADRGQPRHWLRARGGLIKVEGTAGYL
HEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCEEEEECCCCEE
CGIVLDIDQQKVLEQELRLQQSQLRSILDTVPDAMILIDGRGIMRFFSSAAERMFGLSEK
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHH
EAIGQNISVLMPRPDASRHDNYISRYNSTGERHIIGIGRIVTGQRKDGTTFPIHLSIGEM
HHHCCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEEEECCC
ESGGERYFTGFIRDLTEYQQTQARLHELQAELVHVSRLTAMGEMASALAHELNQPLSAIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NYMKGSRRLLADSSDPNVPKIEHAMERAAEQALRAGQIIRRLRDFVARGESEKRVESLAK
HHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LVEEAGALGLTGAREQGVVLRFKLDPASDMALVDRVQIQQVLVNLFRNALEAMANSEKKE
HHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LVVSNARVADQMIEISVADTGSGFHEDVKSSLFQTFFTTKETGMGVGLSISRSIIEAHGG
HHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
RMWAESNPSGGATFRFTLPAAAQEDLADAT
EEEECCCCCCCEEEEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]