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The map label for this gene is fixJ [H]

Identifier: 86748487

GI number: 86748487

Start: 1552839

End: 1553231

Strand: Reverse

Name: fixJ [H]

Synonym: RPB_1362

Alternate gene names: 86748487

Gene position: 1553231-1552839 (Counterclockwise)

Preceding gene: 86748488

Following gene: 86748486

Centisome position: 29.13

GC content: 62.85

Gene sequence:

>393_bases
ATGACCACCGCATCCCCAAAATCTGTTGTCTATGTCGTTGACGACGATCAGGGCGTGCTCGGATCGCTGCGGTTTCTGCT
GGAAACCGACGGGTTCGAAGTATGCACGTTTCGCAGTGGTCCGGCGCTGCTCGATGCGCTCGACGACGGCCGTGCCGATT
GCATCGTGGTTGACTACAAGATGCCCGGGATGAACGGCATCGACCTCGCCCAGAGGCTGCGTGACCGCAAGATCACGACC
CCGGTGGTCATGATCACCGGCTACCCGGACGAGAGCATTTCGAGCCGCGCCGCCGCCGTCGGCGTCCGCCATGTGGTGCT
GAAGCCGCATCTGGAAGAGAGTCTGGTCTTCCACATCCGTGGTGCCTTGCAGGGAAGCGGCGCCCCCGCCTGA

Upstream 100 bases:

>100_bases
GTGATCAGGGATTGAGGCAGATCAACCTGCCTCGCACGCCAGCAACTATTCTCCCCTGCATGAAAGAATTGCAGTCAGGT
CGGGTGCGTGGGACGAACTG

Downstream 100 bases:

>100_bases
CCCGCCTGTGCAGCGGTCTGCGCCGCCCCCTTGATTTCACTGGATTTTCTCTGATCTGCCACGGAGAGCGGCGCCGCCCT
CCGTAAATCTACTTAGGTAA

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MTTASPKSVVYVVDDDQGVLGSLRFLLETDGFEVCTFRSGPALLDALDDGRADCIVVDYKMPGMNGIDLAQRLRDRKITT
PVVMITGYPDESISSRAAAVGVRHVVLKPHLEESLVFHIRGALQGSGAPA

Sequences:

>Translated_130_residues
MTTASPKSVVYVVDDDQGVLGSLRFLLETDGFEVCTFRSGPALLDALDDGRADCIVVDYKMPGMNGIDLAQRLRDRKITT
PVVMITGYPDESISSRAAAVGVRHVVLKPHLEESLVFHIRGALQGSGAPA
>Mature_129_residues
TTASPKSVVYVVDDDQGVLGSLRFLLETDGFEVCTFRSGPALLDALDDGRADCIVVDYKMPGMNGIDLAQRLRDRKITTP
VVMITGYPDESISSRAAAVGVRHVVLKPHLEESLVFHIRGALQGSGAPA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=103, Percent_Identity=31.0679611650485, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13957; Mature: 13826

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTASPKSVVYVVDDDQGVLGSLRFLLETDGFEVCTFRSGPALLDALDDGRADCIVVDYK
CCCCCCCEEEEEEECCCCHHHHEEEEEECCCCEEEEECCCCCEEEECCCCCCCEEEEEEE
MPGMNGIDLAQRLRDRKITTPVVMITGYPDESISSRAAAVGVRHVVLKPHLEESLVFHIR
CCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCEEEEEECCCCCCCEEEEEE
GALQGSGAPA
EEECCCCCCC
>Mature Secondary Structure 
TTASPKSVVYVVDDDQGVLGSLRFLLETDGFEVCTFRSGPALLDALDDGRADCIVVDYK
CCCCCCEEEEEEECCCCHHHHEEEEEECCCCEEEEECCCCCEEEECCCCCCCEEEEEEE
MPGMNGIDLAQRLRDRKITTPVVMITGYPDESISSRAAAVGVRHVVLKPHLEESLVFHIR
CCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCEEEEEECCCCCCCEEEEEE
GALQGSGAPA
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]