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The map label for this gene is nuoE [H]

Identifier: 86748475

GI number: 86748475

Start: 1538622

End: 1539095

Strand: Direct

Name: nuoE [H]

Synonym: RPB_1350

Alternate gene names: 86748475

Gene position: 1538622-1539095 (Clockwise)

Preceding gene: 86748474

Following gene: 86748476

Centisome position: 28.86

GC content: 68.78

Gene sequence:

>474_bases
ATGAGCCTCAGCCCCGCCATCAGGACCGCGATCGCGCAGGCCGCCGCCCATCACGGCGGGCCGAAGGCCGCGATGCTGGA
AGCGCTGAAGTTGGTGCAGGAGGCCGAAGGCTGGGTCTCCGACGCGCATCTGAAGGAAGCAGCAGAGGCGCTCGGCGTGA
CCACCGCCGAGATCGACAGCCTCGCCACTTTCTACAGCCAGATCTTCCGGAGCCCGGTCGGCGATACCGTGATCCTGCTG
TGCGACGGGCTGAGCTGCTTTCTGTGCGGTGGCGACGCCGTGCGCGACGCGGTGATGAGCCGGCTCGGCATCGGCTTCGG
CGAGACCACGCCGGACGGCAAGTTCACGCTGATCAACATCTGCTGCGTCGGCGGCTGCGACCTTGCCCCGGTGGCGCTGG
TCGGGCCGGAGCGCAAGCTGGTCGGGCCGTTGACGGCGGACGATCTCGACGCGCTGATCGGAGGTGCGCGATGA

Upstream 100 bases:

>100_bases
CGCATCTGCAGATGATTTCGGCGGTGGCGCCCGGCATGATGGTGGCCGATCTCGTCGCCTATCTGGGCAGTATCGACTAC
GTGATGTCGGACGTCGACCG

Downstream 100 bases:

>100_bases
GCGACAAGCCGCTCACCGGCCGCGCCACGGCCGATCGTCAGCCGCACAGCCTCGCCGCATGGCGCCGGCTCGGCGGCGGC
GCTGGGTTGGCGCTGGCCTT

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSLSPAIRTAIAQAAAHHGGPKAAMLEALKLVQEAEGWVSDAHLKEAAEALGVTTAEIDSLATFYSQIFRSPVGDTVILL
CDGLSCFLCGGDAVRDAVMSRLGIGFGETTPDGKFTLINICCVGGCDLAPVALVGPERKLVGPLTADDLDALIGGAR

Sequences:

>Translated_157_residues
MSLSPAIRTAIAQAAAHHGGPKAAMLEALKLVQEAEGWVSDAHLKEAAEALGVTTAEIDSLATFYSQIFRSPVGDTVILL
CDGLSCFLCGGDAVRDAVMSRLGIGFGETTPDGKFTLINICCVGGCDLAPVALVGPERKLVGPLTADDLDALIGGAR
>Mature_156_residues
SLSPAIRTAIAQAAAHHGGPKAAMLEALKLVQEAEGWVSDAHLKEAAEALGVTTAEIDSLATFYSQIFRSPVGDTVILLC
DGLSCFLCGGDAVRDAVMSRLGIGFGETTPDGKFTLINICCVGGCDLAPVALVGPERKLVGPLTADDLDALIGGAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=135, Percent_Identity=37.037037037037, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788621, Length=134, Percent_Identity=43.2835820895522, Blast_Score=126, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17561328, Length=136, Percent_Identity=31.6176470588235, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI18859877, Length=137, Percent_Identity=31.3868613138686, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355353, Length=114, Percent_Identity=35.9649122807018, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 16110; Mature: 15979

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSPAIRTAIAQAAAHHGGPKAAMLEALKLVQEAEGWVSDAHLKEAAEALGVTTAEIDS
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LATFYSQIFRSPVGDTVILLCDGLSCFLCGGDAVRDAVMSRLGIGFGETTPDGKFTLINI
HHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
CCVGGCDLAPVALVGPERKLVGPLTADDLDALIGGAR
EEECCCCCCCEEEECCCCHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SLSPAIRTAIAQAAAHHGGPKAAMLEALKLVQEAEGWVSDAHLKEAAEALGVTTAEIDS
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LATFYSQIFRSPVGDTVILLCDGLSCFLCGGDAVRDAVMSRLGIGFGETTPDGKFTLINI
HHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
CCVGGCDLAPVALVGPERKLVGPLTADDLDALIGGAR
EEECCCCCCCEEEECCCCHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]