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The map label for this gene is nuoF [H]

Identifier: 86748476

GI number: 86748476

Start: 1539092

End: 1540378

Strand: Direct

Name: nuoF [H]

Synonym: RPB_1351

Alternate gene names: 86748476

Gene position: 1539092-1540378 (Clockwise)

Preceding gene: 86748475

Following gene: 86748477

Centisome position: 28.87

GC content: 70.16

Gene sequence:

>1287_bases
ATGAGCGACAAGCCGCTCACCGGCCGCGCCACGGCCGATCGTCAGCCGCACAGCCTCGCCGCATGGCGCCGGCTCGGCGG
CGGCGCTGGGTTGGCGCTGGCCTTGAAGCGCCACAGCCCCGAGGCGATCATCGCGCTGATCGAAGCCGCGGGTTTGCGTG
GTCGCGGCGGCGCGGGATTCCCGACCGCCAACAAATGGCGCTTCATGCGCGCCGGTGCCGAGAAGGCCGGGCCGGGCGCG
TGCTATCTCTGCGTCAACGGCGACGAGACCGAGCCCGGATCGTTCAAGGACCGCCTGCTGATGGAGGCGTTGCCACAACA
ACTGATCGAGGGCGCCACGATCGCCGCCTATGCGATGGGCGCAACCGAAGTGATCATCCTGGTGCGCGACGAATATCGCG
CCGCCGCCGCCGCGCTCTCCCGCGCCATCGTCGAGGCCGAACAGACCGGCCTGCTCGGCCGCGACATTCTCGGCTCGGGC
TTCGATCTGACGATGCGGGTGCACGCCTCGGCCGGCCGCTATATCGTCGGCGAGGAGACCGCGCTGATCGCCGCGCTGGA
AGGCGAACGTCCCGTGCCGCGGCATCGCCCGCCTTATCCCGCCGTCAGCGGGCTGTGGGGTCGGCCGACCACGGTCAACA
ATGTCGAGACGCTGTCGGCGGTGCCCTCGATCGTCGAGCACGGCGCCGAGTGGTATCAGGGTTTGTCGCACACCAACGAA
GGCGGCACCAAGCTGTACGGCGTCTCCGGCTGCGTCGCGCGTCCGCAACTGATCGAGGCGCCGATGGGCACCACCGCACG
CGAATTGTTCGATCTGTGCGGCGGCATTCGTCAAGGCCGCGCGCTGTTCGCCTTCCAGCCCGGCGGTGGCGCCACGGCGT
TTCTGGAGACGGCCGAGCTCGACGTGCCGCTCGACTTCGCGCACACCAAGAGGGCCGGCTCTTCGCTCGGCACCGGCGCG
CTGATCGTGCTGGACGACCACGCCTGTCCGGTGGCGGCGATCGGCCGCCACATGCGGTTCTATGCGCGCGAGAGCTGCGG
GCTGTGCACGCCCTGTCGCGACGGGCTGCCGTGGGTGGCGAAGCTGCTCGACACGATCGAGGCCGGCAAGGGCACGCGCG
GCGATATCGACCTGCTGCACCAGCACGTCGCGCTGTCCGGCCCGTCCGGCCGCTCCTATTGCGACCTCAACACCGGCGCG
CTGACGCCGCTGCGCAGCGGCCTCGAACGCTTCGGCGATATCTTCGCTGCCCATCTGCAGGGCTCCTGTCCGGTGGGCCG
CGCATGA

Upstream 100 bases:

>100_bases
GCGGCTGCGACCTTGCCCCGGTGGCGCTGGTCGGGCCGGAGCGCAAGCTGGTCGGGCCGTTGACGGCGGACGATCTCGAC
GCGCTGATCGGAGGTGCGCG

Downstream 100 bases:

>100_bases
CCAAGAAGATCGAGATCGACGGCCGCATCATCGAGGCCAAGGACGACGAGGACCTGTTGTCGGCATGCCTGACGCACGGG
GTCGATGTGCCGTATTTCTG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSDKPLTGRATADRQPHSLAAWRRLGGGAGLALALKRHSPEAIIALIEAAGLRGRGGAGFPTANKWRFMRAGAEKAGPGA
CYLCVNGDETEPGSFKDRLLMEALPQQLIEGATIAAYAMGATEVIILVRDEYRAAAAALSRAIVEAEQTGLLGRDILGSG
FDLTMRVHASAGRYIVGEETALIAALEGERPVPRHRPPYPAVSGLWGRPTTVNNVETLSAVPSIVEHGAEWYQGLSHTNE
GGTKLYGVSGCVARPQLIEAPMGTTARELFDLCGGIRQGRALFAFQPGGGATAFLETAELDVPLDFAHTKRAGSSLGTGA
LIVLDDHACPVAAIGRHMRFYARESCGLCTPCRDGLPWVAKLLDTIEAGKGTRGDIDLLHQHVALSGPSGRSYCDLNTGA
LTPLRSGLERFGDIFAAHLQGSCPVGRA

Sequences:

>Translated_428_residues
MSDKPLTGRATADRQPHSLAAWRRLGGGAGLALALKRHSPEAIIALIEAAGLRGRGGAGFPTANKWRFMRAGAEKAGPGA
CYLCVNGDETEPGSFKDRLLMEALPQQLIEGATIAAYAMGATEVIILVRDEYRAAAAALSRAIVEAEQTGLLGRDILGSG
FDLTMRVHASAGRYIVGEETALIAALEGERPVPRHRPPYPAVSGLWGRPTTVNNVETLSAVPSIVEHGAEWYQGLSHTNE
GGTKLYGVSGCVARPQLIEAPMGTTARELFDLCGGIRQGRALFAFQPGGGATAFLETAELDVPLDFAHTKRAGSSLGTGA
LIVLDDHACPVAAIGRHMRFYARESCGLCTPCRDGLPWVAKLLDTIEAGKGTRGDIDLLHQHVALSGPSGRSYCDLNTGA
LTPLRSGLERFGDIFAAHLQGSCPVGRA
>Mature_427_residues
SDKPLTGRATADRQPHSLAAWRRLGGGAGLALALKRHSPEAIIALIEAAGLRGRGGAGFPTANKWRFMRAGAEKAGPGAC
YLCVNGDETEPGSFKDRLLMEALPQQLIEGATIAAYAMGATEVIILVRDEYRAAAAALSRAIVEAEQTGLLGRDILGSGF
DLTMRVHASAGRYIVGEETALIAALEGERPVPRHRPPYPAVSGLWGRPTTVNNVETLSAVPSIVEHGAEWYQGLSHTNEG
GTKLYGVSGCVARPQLIEAPMGTTARELFDLCGGIRQGRALFAFQPGGGATAFLETAELDVPLDFAHTKRAGSSLGTGAL
IVLDDHACPVAAIGRHMRFYARESCGLCTPCRDGLPWVAKLLDTIEAGKGTRGDIDLLHQHVALSGPSGRSYCDLNTGAL
TPLRSGLERFGDIFAAHLQGSCPVGRA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=375, Percent_Identity=40.2666666666667, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI20149568, Length=375, Percent_Identity=40.2666666666667, Blast_Score=261, Evalue=6e-70,
Organism=Escherichia coli, GI1788620, Length=418, Percent_Identity=49.5215311004785, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17531727, Length=373, Percent_Identity=41.0187667560322, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=41.8666666666667, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI45550452, Length=381, Percent_Identity=39.6325459317585, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24653819, Length=346, Percent_Identity=35.8381502890173, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24653817, Length=317, Percent_Identity=34.384858044164, Blast_Score=188, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 45092; Mature: 44961

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKPLTGRATADRQPHSLAAWRRLGGGAGLALALKRHSPEAIIALIEAAGLRGRGGAGF
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
PTANKWRFMRAGAEKAGPGACYLCVNGDETEPGSFKDRLLMEALPQQLIEGATIAAYAMG
CCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECC
ATEVIILVRDEYRAAAAALSRAIVEAEQTGLLGRDILGSGFDLTMRVHASAGRYIVGEET
CEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCEEECCCE
ALIAALEGERPVPRHRPPYPAVSGLWGRPTTVNNVETLSAVPSIVEHGAEWYQGLSHTNE
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GGTKLYGVSGCVARPQLIEAPMGTTARELFDLCGGIRQGRALFAFQPGGGATAFLETAEL
CCCEEEECCCCCCCCHHHCCCCCCHHHHHHHHHCCHHCCCEEEEEECCCCCEEEEEECCC
DVPLDFAHTKRAGSSLGTGALIVLDDHACPVAAIGRHMRFYARESCGLCTPCRDGLPWVA
CCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
KLLDTIEAGKGTRGDIDLLHQHVALSGPSGRSYCDLNTGALTPLRSGLERFGDIFAAHLQ
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
GSCPVGRA
CCCCCCCC
>Mature Secondary Structure 
SDKPLTGRATADRQPHSLAAWRRLGGGAGLALALKRHSPEAIIALIEAAGLRGRGGAGF
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
PTANKWRFMRAGAEKAGPGACYLCVNGDETEPGSFKDRLLMEALPQQLIEGATIAAYAMG
CCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECC
ATEVIILVRDEYRAAAAALSRAIVEAEQTGLLGRDILGSGFDLTMRVHASAGRYIVGEET
CEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCEEECCCE
ALIAALEGERPVPRHRPPYPAVSGLWGRPTTVNNVETLSAVPSIVEHGAEWYQGLSHTNE
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GGTKLYGVSGCVARPQLIEAPMGTTARELFDLCGGIRQGRALFAFQPGGGATAFLETAEL
CCCEEEECCCCCCCCHHHCCCCCCHHHHHHHHHCCHHCCCEEEEEECCCCCEEEEEECCC
DVPLDFAHTKRAGSSLGTGALIVLDDHACPVAAIGRHMRFYARESCGLCTPCRDGLPWVA
CCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
KLLDTIEAGKGTRGDIDLLHQHVALSGPSGRSYCDLNTGALTPLRSGLERFGDIFAAHLQ
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
GSCPVGRA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]