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The map label for this gene is truA [H]

Identifier: 86747801

GI number: 86747801

Start: 759551

End: 760288

Strand: Direct

Name: truA [H]

Synonym: RPB_0675

Alternate gene names: 86747801

Gene position: 759551-760288 (Clockwise)

Preceding gene: 86747800

Following gene: 86747812

Centisome position: 14.25

GC content: 69.38

Gene sequence:

>738_bases
ATGCCCCGCTACAAACTCACCATCGAATATGACGGCGCGCCGTTTTGCGGCTGGCAGTTGCAGCCGACGTTGCCGTCGGT
GCAGGGCGCGCTGGAGGCGGCGGCGCTGGCGACCTGCGGCGAAGCGGTGCGGGTGCACGGCGCCGGCCGCACCGATGCGG
GCGTGCACGCGCTGGGGCAGGTGGCGCATGTCGACATTCCGAAGCCGTTCCGCGCCGACAAGCTGCGCGACGCGCTCAAC
GCCCATGTGCGGCCGCATCCGATCGCGGTCCTGTCCGCCGAACTCGTGGCGGATGATTTCGAGGCGCGGTTCTCGGCGAT
CCGGCGGCACTACCGCTATCGCATCGTCAACCGCCGCTCCAATCTCGCGCTCGAGCTCGGCAAAGTGTGGCGGGTGCCGA
AGCCACTCGACACCGACGCGATGCACCGCGCAGCGCAAGTGCTGATCGGCAAGCACGACTTCACGACGTTCCGCGACACC
GAGTGCCAGGCCGCCTCGCCGGCCAAGACGCTGGACGTGCTCGACGTCGTGCGGAACGGCGACGCCGTCGATATCATCAC
CAATGCGCGTTCCTATCTGCACAGCCAGGTGCGCTCGATGGTCGGCTCCCTGGTGTGGGTCGGCGAAGGCCGCTGGACGG
CGGATGATCTCGCCGCCGCGCTCGCCGCCCGCCGCCGCTCCGCCTGCGGCCCGGTGGCGCCGCCGGATGGGTTGTATCTG
GTGCAGGTGGATTACTGA

Upstream 100 bases:

>100_bases
CTGCAATACTGGGTCGCCCGGTCGAGCCGGGCGATGACGTGGTGGTGATGGGCTTCGCTCCCGCTCCAGCACCGCGCGCG
GTCGCAGAAACAAGAGAAGC

Downstream 100 bases:

>100_bases
GTACTAGAGCTGTTCATCGCTTGATTGAAGCGGCGCGGCATTCTCGAATGCGGAGTCGTCATCCTGAGGTGCGAGCGCAG
CGAGCCTCGAAGGATGCGGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPRYKLTIEYDGAPFCGWQLQPTLPSVQGALEAAALATCGEAVRVHGAGRTDAGVHALGQVAHVDIPKPFRADKLRDALN
AHVRPHPIAVLSAELVADDFEARFSAIRRHYRYRIVNRRSNLALELGKVWRVPKPLDTDAMHRAAQVLIGKHDFTTFRDT
ECQAASPAKTLDVLDVVRNGDAVDIITNARSYLHSQVRSMVGSLVWVGEGRWTADDLAAALAARRRSACGPVAPPDGLYL
VQVDY

Sequences:

>Translated_245_residues
MPRYKLTIEYDGAPFCGWQLQPTLPSVQGALEAAALATCGEAVRVHGAGRTDAGVHALGQVAHVDIPKPFRADKLRDALN
AHVRPHPIAVLSAELVADDFEARFSAIRRHYRYRIVNRRSNLALELGKVWRVPKPLDTDAMHRAAQVLIGKHDFTTFRDT
ECQAASPAKTLDVLDVVRNGDAVDIITNARSYLHSQVRSMVGSLVWVGEGRWTADDLAAALAARRRSACGPVAPPDGLYL
VQVDY
>Mature_244_residues
PRYKLTIEYDGAPFCGWQLQPTLPSVQGALEAAALATCGEAVRVHGAGRTDAGVHALGQVAHVDIPKPFRADKLRDALNA
HVRPHPIAVLSAELVADDFEARFSAIRRHYRYRIVNRRSNLALELGKVWRVPKPLDTDAMHRAAQVLIGKHDFTTFRDTE
CQAASPAKTLDVLDVVRNGDAVDIITNARSYLHSQVRSMVGSLVWVGEGRWTADDLAAALAARRRSACGPVAPPDGLYLV
QVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=35.2727272727273, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=42.6829268292683, Blast_Score=174, Evalue=3e-45,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=30.4635761589404, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 26835; Mature: 26704

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYKLTIEYDGAPFCGWQLQPTLPSVQGALEAAALATCGEAVRVHGAGRTDAGVHALGQ
CCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHHHH
VAHVDIPKPFRADKLRDALNAHVRPHPIAVLSAELVADDFEARFSAIRRHYRYRIVNRRS
HEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
NLALELGKVWRVPKPLDTDAMHRAAQVLIGKHDFTTFRDTECQAASPAKTLDVLDVVRNG
CCEEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
DAVDIITNARSYLHSQVRSMVGSLVWVGEGRWTADDLAAALAARRRSACGPVAPPDGLYL
CEEEEHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
VQVDY
EEECC
>Mature Secondary Structure 
PRYKLTIEYDGAPFCGWQLQPTLPSVQGALEAAALATCGEAVRVHGAGRTDAGVHALGQ
CCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHHHH
VAHVDIPKPFRADKLRDALNAHVRPHPIAVLSAELVADDFEARFSAIRRHYRYRIVNRRS
HEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
NLALELGKVWRVPKPLDTDAMHRAAQVLIGKHDFTTFRDTECQAASPAKTLDVLDVVRNG
CCEEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
DAVDIITNARSYLHSQVRSMVGSLVWVGEGRWTADDLAAALAARRRSACGPVAPPDGLYL
CEEEEHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
VQVDY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA