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The map label for this gene is ntrB [H]

Identifier: 86747812

GI number: 86747812

Start: 769550

End: 771037

Strand: Direct

Name: ntrB [H]

Synonym: RPB_0686

Alternate gene names: 86747812

Gene position: 769550-771037 (Clockwise)

Preceding gene: 86747801

Following gene: 86747817

Centisome position: 14.43

GC content: 67.47

Gene sequence:

>1488_bases
GTGCAGGCAGCGGATCAACAGCCTGAAACGCGGGCATGGCGGCGAGGCCTGGAGCCGAAGATCGGCTTGTCCGGGAAGCT
GCTGCTGTTGACGATTCCGCTGATCCTGATCGCCGAAATCCTGATCTACGTGCCGTCAATCGCCAATTTCCGCCTGAACC
GGCTCAACGACCGGCTTGCGGCTGCCAATACGGCCGCGCTGGTCCTGGACGCCTCGCCCTCCGGCATGGTGCCGGATACG
CTGGCGCGCCAGGTGCTGGACAGCATCGACGCCCGCGCGGTGGCGATCAAAATGGGCCAGCAGCGTCGCCTGCTGGCAAC
CTCGAACCTGCCCAGCACGATCGACCAGGTCGTCGATATGCGCCACGTCACGCCGTGGGGCGCGATCATCGATGCCTTTG
CGGTGATGCTGGAGAGCGGCAACCAGACCGTCCGGGTGATCGGCCCGGCGGAGGGCAACGCGCAGTTCATCGAAGTCGTG
ATCGACGAAGCGCCGCTGCGGATCGCCATGTACCGCTTCTCCAGCAATCTGCTGCTGGTGTCGTTGACCATCACCAGCCT
GACCACCGCGCTGATCTATCTGGCGCTGCATTTCCTGTTCGTGCGGCCGATGCGGCGGCTGACCGCCAACATGGTCAATT
TCCGCCGCGATCCGGAAAGCCCGGCTTCGATCGTGATCCCGGGCCCGCGCGGCGACGAAATCGGCCTCGCCGAGCGCGAA
TTGTCCGACATGCAGCGCGATCTGGTGTCGATGCTGCATCAGAAGAGCCGGCTCGCCGCGCTCGGCCTCGCGGTGTCGAA
GATCAATCACGATCTGCGCAATTTGCTGGCGTCGTCGCAGCTGCTGTCCGACCAGCTCTCCAGCGTGCCCGACCCGCGGG
TGCAGCGCTTCGCGCCGAAGCTGGTGCGCTCGCTGGAGCGGGCGATCGCGTTCTGCCAGTCGACGCTGTCCTATGGCCGC
GCCCAGGAGGCCGCACCGGACCGCCGGATGATCCTGATCGAACCGGTGGTCAACGAGGTCCGCGAGACCGCCGGGCTCGC
CTCCGACACGGCCGTCAGCTCGGTCACCTGGGTCTCGGCGATCGAGCGCGGGCTGACCATGGACGCCGACCCTGATCAAT
TGTTCCGGGTGCTGCTGAACCTGGTGCGCAACGCCGCGCAGGCGCTGGAAAGCCAGCCGCGCAGCGACGCCGCCTTGCAG
CAGATCCGGATCACCGGCCGTCGCGAGGGGTCGGTGACGATTCTGGAAGTGTCCGACACCGGCCCCGGCGTGCCGCAAAA
GGCCCGCGACCATCTGTTCGAGGCGTTCCACGGCTCGGTCCGGGCCGGCGGCTCGGGTCTCGGGCTGGCGATCGCCGCCG
AGCTGGTCCGCGCCCATGGCGGCGACATCAAGCTGGTCGAGGGAACGCTGGGCGCGACCTTCCGGATCTCGATTCCGGAC
CGGCCGGTCGACCTGCACAGCCTGCGCAACGAACGCGCCAGGGCCTGA

Upstream 100 bases:

>100_bases
ATGATCGAACGGTAATTTGACCTGGCGGTGTTCAGGGCGCATTTTGAGACATATATTCTTTGCGCCGAATCCGGAGGGAT
CGGAAGTCAGGGCAAATCGG

Downstream 100 bases:

>100_bases
TCCCGGCTTTGGGTCTGATTCCGCCTTGCGCCTGATCCAGCCTGGGGCTCGATCGAGCCCGATCGAGCTTTCGTTAGGTC
TGGCCCCGCCTTGTCCCGAT

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MQAADQQPETRAWRRGLEPKIGLSGKLLLLTIPLILIAEILIYVPSIANFRLNRLNDRLAAANTAALVLDASPSGMVPDT
LARQVLDSIDARAVAIKMGQQRRLLATSNLPSTIDQVVDMRHVTPWGAIIDAFAVMLESGNQTVRVIGPAEGNAQFIEVV
IDEAPLRIAMYRFSSNLLLVSLTITSLTTALIYLALHFLFVRPMRRLTANMVNFRRDPESPASIVIPGPRGDEIGLAERE
LSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLSSVPDPRVQRFAPKLVRSLERAIAFCQSTLSYGR
AQEAAPDRRMILIEPVVNEVRETAGLASDTAVSSVTWVSAIERGLTMDADPDQLFRVLLNLVRNAAQALESQPRSDAALQ
QIRITGRREGSVTILEVSDTGPGVPQKARDHLFEAFHGSVRAGGSGLGLAIAAELVRAHGGDIKLVEGTLGATFRISIPD
RPVDLHSLRNERARA

Sequences:

>Translated_495_residues
MQAADQQPETRAWRRGLEPKIGLSGKLLLLTIPLILIAEILIYVPSIANFRLNRLNDRLAAANTAALVLDASPSGMVPDT
LARQVLDSIDARAVAIKMGQQRRLLATSNLPSTIDQVVDMRHVTPWGAIIDAFAVMLESGNQTVRVIGPAEGNAQFIEVV
IDEAPLRIAMYRFSSNLLLVSLTITSLTTALIYLALHFLFVRPMRRLTANMVNFRRDPESPASIVIPGPRGDEIGLAERE
LSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLSSVPDPRVQRFAPKLVRSLERAIAFCQSTLSYGR
AQEAAPDRRMILIEPVVNEVRETAGLASDTAVSSVTWVSAIERGLTMDADPDQLFRVLLNLVRNAAQALESQPRSDAALQ
QIRITGRREGSVTILEVSDTGPGVPQKARDHLFEAFHGSVRAGGSGLGLAIAAELVRAHGGDIKLVEGTLGATFRISIPD
RPVDLHSLRNERARA
>Mature_495_residues
MQAADQQPETRAWRRGLEPKIGLSGKLLLLTIPLILIAEILIYVPSIANFRLNRLNDRLAAANTAALVLDASPSGMVPDT
LARQVLDSIDARAVAIKMGQQRRLLATSNLPSTIDQVVDMRHVTPWGAIIDAFAVMLESGNQTVRVIGPAEGNAQFIEVV
IDEAPLRIAMYRFSSNLLLVSLTITSLTTALIYLALHFLFVRPMRRLTANMVNFRRDPESPASIVIPGPRGDEIGLAERE
LSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLSSVPDPRVQRFAPKLVRSLERAIAFCQSTLSYGR
AQEAAPDRRMILIEPVVNEVRETAGLASDTAVSSVTWVSAIERGLTMDADPDQLFRVLLNLVRNAAQALESQPRSDAALQ
QIRITGRREGSVTILEVSDTGPGVPQKARDHLFEAFHGSVRAGGSGLGLAIAAELVRAHGGDIKLVEGTLGATFRISIPD
RPVDLHSLRNERARA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=28.4518828451883, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790300, Length=249, Percent_Identity=28.9156626506024, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790436, Length=115, Percent_Identity=35.6521739130435, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53944; Mature: 53944

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAADQQPETRAWRRGLEPKIGLSGKLLLLTIPLILIAEILIYVPSIANFRLNRLNDRLA
CCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AANTAALVLDASPSGMVPDTLARQVLDSIDARAVAIKMGQQRRLLATSNLPSTIDQVVDM
HCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEECCCCCCCEEECCCCCHHHHHHHHH
RHVTPWGAIIDAFAVMLESGNQTVRVIGPAEGNAQFIEVVIDEAPLRIAMYRFSSNLLLV
HCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCEEEE
SLTITSLTTALIYLALHFLFVRPMRRLTANMVNFRRDPESPASIVIPGPRGDEIGLAERE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHH
LSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLSSVPDPRVQRFAPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LVRSLERAIAFCQSTLSYGRAQEAAPDRRMILIEPVVNEVRETAGLASDTAVSSVTWVSA
HHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHH
IERGLTMDADPDQLFRVLLNLVRNAAQALESQPRSDAALQQIRITGRREGSVTILEVSDT
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCC
GPGVPQKARDHLFEAFHGSVRAGGSGLGLAIAAELVRAHGGDIKLVEGTLGATFRISIPD
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCC
RPVDLHSLRNERARA
CCCHHHHHHHHHCCC
>Mature Secondary Structure
MQAADQQPETRAWRRGLEPKIGLSGKLLLLTIPLILIAEILIYVPSIANFRLNRLNDRLA
CCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AANTAALVLDASPSGMVPDTLARQVLDSIDARAVAIKMGQQRRLLATSNLPSTIDQVVDM
HCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEECCCCCCCEEECCCCCHHHHHHHHH
RHVTPWGAIIDAFAVMLESGNQTVRVIGPAEGNAQFIEVVIDEAPLRIAMYRFSSNLLLV
HCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCEEEE
SLTITSLTTALIYLALHFLFVRPMRRLTANMVNFRRDPESPASIVIPGPRGDEIGLAERE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHH
LSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLSSVPDPRVQRFAPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LVRSLERAIAFCQSTLSYGRAQEAAPDRRMILIEPVVNEVRETAGLASDTAVSSVTWVSA
HHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHH
IERGLTMDADPDQLFRVLLNLVRNAAQALESQPRSDAALQQIRITGRREGSVTILEVSDT
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCC
GPGVPQKARDHLFEAFHGSVRAGGSGLGLAIAAELVRAHGGDIKLVEGTLGATFRISIPD
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCC
RPVDLHSLRNERARA
CCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]