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The map label for this gene is fmt

Identifier: 86747800

GI number: 86747800

Start: 758423

End: 759361

Strand: Direct

Name: fmt

Synonym: RPB_0674

Alternate gene names: 86747800

Gene position: 758423-759361 (Clockwise)

Preceding gene: 86747799

Following gene: 86747801

Centisome position: 14.22

GC content: 70.07

Gene sequence:

>939_bases
ATGCCTCTTCGCCTCGTTTTCATGGGCACGCCCGATTTCGCGGTGCCGACGCTGCTGGCGCTGTCGGCCTATGGGCACGA
CATCGCGGCGGTCTATAGCCGCGAGCCGAAGCCGGCCGGGCGCGGCATGAAACTGCAGCACAGCCCGGTGGCGCAGGAGG
CGCAGCGGCTCGGGATTCCCGTGCTGACGCCGAAGACGCTGAGAACCGACGAGGCGTTGGCCGAATTCCGCAGCCACGAG
GCCGACGCGGCGGTGGTGGTCGCCTACGGCATGATCCTGCCGCAGGCGATCCTCGATGCACCAAAGCTCGGCTGCTACAA
TCTGCACGGCTCGCTGCTGCCGCGCTGGCGCGGCGCCGCGCCGCTCAACCGCGCCATCATGGCGGGCGACGCCGAATCGG
GCGTGATGGTGATGAAGATGGACGTCGGCCTCGACACCGGCGACGTCGCGATGGCCGAGCGGATCGCCATCACCGATGCG
ATGACCGTCACCGATCTGCACGACGCGCTGGCACGGCTCGGCGCCGACCTGATGGTGCGGGCGATGGCGGCGCTGGAGCG
CGGCGGGCTGCAGCTCGCCCGGCAGAGCGAACACGGCGTCAGCTACGCGGCCAAGATCGACAAGGCGGAAGCGAAGATCG
ATTTCGCCCGGCCGGCGCAGGCGGTGCTGCGCCACATTCACGGGCTGTCGCCGTTTCCCGGCGCGTGGTGCGAACTGCCG
ATCGACGGCGAGGCGGTGCGGGTCAAGATCCTGCGCTGCGCGCTCGCCGATGGTCGCGGCGCTCCGGGCGAGATGCTGGG
CGACGATCTCACCATCGCCTGCGCCGACGGCGCGATCCGCGTGCTGCAATTGCAGCGCGCCGGCAAGCAGCCGATGAGCG
CCGACGAGTTCCTGCGCGGCACGCCGATCGCGAAGGGCGTGCGGGTGAGTTCAAGCTGA

Upstream 100 bases:

>100_bases
CCAAGCGCGGCATCAAATACGTCTGAGCGTTCGCGCAGACGACCGGCCGACGCGTTGCGGCGCTCGGCCCTTCGTCGGCC
CAGACCTGGGACCTCACGCC

Downstream 100 bases:

>100_bases
GGCGGGGCGCCGCCCCACCCACAGCCGTCACCACCCGCGCATGCGGGTGGCCCAGTATCCCAGAGCGTTCATGATTGATA
ACCAGCGCTCTGCAATACTG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPLRLVFMGTPDFAVPTLLALSAYGHDIAAVYSREPKPAGRGMKLQHSPVAQEAQRLGIPVLTPKTLRTDEALAEFRSHE
ADAAVVVAYGMILPQAILDAPKLGCYNLHGSLLPRWRGAAPLNRAIMAGDAESGVMVMKMDVGLDTGDVAMAERIAITDA
MTVTDLHDALARLGADLMVRAMAALERGGLQLARQSEHGVSYAAKIDKAEAKIDFARPAQAVLRHIHGLSPFPGAWCELP
IDGEAVRVKILRCALADGRGAPGEMLGDDLTIACADGAIRVLQLQRAGKQPMSADEFLRGTPIAKGVRVSSS

Sequences:

>Translated_312_residues
MPLRLVFMGTPDFAVPTLLALSAYGHDIAAVYSREPKPAGRGMKLQHSPVAQEAQRLGIPVLTPKTLRTDEALAEFRSHE
ADAAVVVAYGMILPQAILDAPKLGCYNLHGSLLPRWRGAAPLNRAIMAGDAESGVMVMKMDVGLDTGDVAMAERIAITDA
MTVTDLHDALARLGADLMVRAMAALERGGLQLARQSEHGVSYAAKIDKAEAKIDFARPAQAVLRHIHGLSPFPGAWCELP
IDGEAVRVKILRCALADGRGAPGEMLGDDLTIACADGAIRVLQLQRAGKQPMSADEFLRGTPIAKGVRVSSS
>Mature_311_residues
PLRLVFMGTPDFAVPTLLALSAYGHDIAAVYSREPKPAGRGMKLQHSPVAQEAQRLGIPVLTPKTLRTDEALAEFRSHEA
DAAVVVAYGMILPQAILDAPKLGCYNLHGSLLPRWRGAAPLNRAIMAGDAESGVMVMKMDVGLDTGDVAMAERIAITDAM
TVTDLHDALARLGADLMVRAMAALERGGLQLARQSEHGVSYAAKIDKAEAKIDFARPAQAVLRHIHGLSPFPGAWCELPI
DGEAVRVKILRCALADGRGAPGEMLGDDLTIACADGAIRVLQLQRAGKQPMSADEFLRGTPIAKGVRVSSS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=242, Percent_Identity=28.9256198347107, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI238814322, Length=310, Percent_Identity=29.3548387096774, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI164663775, Length=319, Percent_Identity=26.3322884012539, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789683, Length=300, Percent_Identity=44, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1788589, Length=312, Percent_Identity=25.3205128205128, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI133930964, Length=258, Percent_Identity=27.906976744186, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319458, Length=326, Percent_Identity=26.0736196319018, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550868, Length=312, Percent_Identity=30.7692307692308, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571984, Length=234, Percent_Identity=33.7606837606838, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_RHOP2 (Q2J2C5)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484296.1
- ProteinModelPortal:   Q2J2C5
- SMR:   Q2J2C5
- STRING:   Q2J2C5
- GeneID:   3908601
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0674
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   RPAL316058:RPB_0674-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33153; Mature: 33022

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRLVFMGTPDFAVPTLLALSAYGHDIAAVYSREPKPAGRGMKLQHSPVAQEAQRLGIP
CCEEEEEECCCCHHHHHHHHHHHCCCCEEEHCCCCCCCCCCCCEECCCCHHHHHHHCCCC
VLTPKTLRTDEALAEFRSHEADAAVVVAYGMILPQAILDAPKLGCYNLHGSLLPRWRGAA
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PLNRAIMAGDAESGVMVMKMDVGLDTGDVAMAERIAITDAMTVTDLHDALARLGADLMVR
CCCHHEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMAALERGGLQLARQSEHGVSYAAKIDKAEAKIDFARPAQAVLRHIHGLSPFPGAWCELP
HHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCEECC
IDGEAVRVKILRCALADGRGAPGEMLGDDLTIACADGAIRVLQLQRAGKQPMSADEFLRG
CCCCHHEEEEEEHHHCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHCC
TPIAKGVRVSSS
CCCCCCEEECCC
>Mature Secondary Structure 
PLRLVFMGTPDFAVPTLLALSAYGHDIAAVYSREPKPAGRGMKLQHSPVAQEAQRLGIP
CEEEEEECCCCHHHHHHHHHHHCCCCEEEHCCCCCCCCCCCCEECCCCHHHHHHHCCCC
VLTPKTLRTDEALAEFRSHEADAAVVVAYGMILPQAILDAPKLGCYNLHGSLLPRWRGAA
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PLNRAIMAGDAESGVMVMKMDVGLDTGDVAMAERIAITDAMTVTDLHDALARLGADLMVR
CCCHHEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMAALERGGLQLARQSEHGVSYAAKIDKAEAKIDFARPAQAVLRHIHGLSPFPGAWCELP
HHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCEECC
IDGEAVRVKILRCALADGRGAPGEMLGDDLTIACADGAIRVLQLQRAGKQPMSADEFLRG
CCCCHHEEEEEEHHHCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHCC
TPIAKGVRVSSS
CCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA