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Identifier: 86747799

GI number: 86747799

Start: 757821

End: 758348

Strand: Direct

Name: def

Synonym: RPB_0673

Alternate gene names: 86747799

Gene position: 757821-758348 (Clockwise)

Preceding gene: 86747797

Following gene: 86747800

Centisome position: 14.21

GC content: 62.12

Gene sequence:

>528_bases
ATGGCCCTGCGCGAAATCATCATCCTGCCGGACAAGCGGCTGCGTGAAATCTCCAAGCCCGTCGCCGAGGTGACGCCGGA
GATCCGCAAGCTCGCGGACGACATGTTCGAGAGCATGTACGAGGCGCCCGGCATCGGGCTGGCGGCGATTCAGATCGCCG
AGCCGGTGCGGCTGATCACGATGGACATCGTGCGCAAAGAGGGCAACGGCAAGAGCGATCCGCGGGCCTTCATCAATCCG
GAGATCGTCGGCGCCTCGGCCGAGCTGAACGTCTACGAGGAAGGCTGCCTGTCGATCCCGGAATACTACGCCGAGGTCGA
GCGGCCCAAGACGGTGCGGATTCGTTACACCGATCTCGACGGTCAGGTGAAGGAAGAGGACGCCGACGGCCTGTTCGCGA
CCTGCATCCAGCACGAGATCGACCACCTCAACGGCGTGCTGTTCGTCGATCATATCTCGAAGCTGAAAAAGGCGATGGTC
GTCCGCAAGTTCGAGAAGGCCGCCAAGCGCGGCATCAAATACGTCTGA

Upstream 100 bases:

>100_bases
TCACGCTGAACAGATCTTTAAGCGGGCCCGCGCCGAAAGTGGCAGATCCTCCGGTCGGTCCCGGATTGACCCGCAAGCGC
GCGCGCCTTAAATCGCCGCC

Downstream 100 bases:

>100_bases
GCGTTCGCGCAGACGACCGGCCGACGCGTTGCGGCGCTCGGCCCTTCGTCGGCCCAGACCTGGGACCTCACGCCATGCCT
CTTCGCCTCGTTTTCATGGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MALREIIILPDKRLREISKPVAEVTPEIRKLADDMFESMYEAPGIGLAAIQIAEPVRLITMDIVRKEGNGKSDPRAFINP
EIVGASAELNVYEEGCLSIPEYYAEVERPKTVRIRYTDLDGQVKEEDADGLFATCIQHEIDHLNGVLFVDHISKLKKAMV
VRKFEKAAKRGIKYV

Sequences:

>Translated_175_residues
MALREIIILPDKRLREISKPVAEVTPEIRKLADDMFESMYEAPGIGLAAIQIAEPVRLITMDIVRKEGNGKSDPRAFINP
EIVGASAELNVYEEGCLSIPEYYAEVERPKTVRIRYTDLDGQVKEEDADGLFATCIQHEIDHLNGVLFVDHISKLKKAMV
VRKFEKAAKRGIKYV
>Mature_174_residues
ALREIIILPDKRLREISKPVAEVTPEIRKLADDMFESMYEAPGIGLAAIQIAEPVRLITMDIVRKEGNGKSDPRAFINPE
IVGASAELNVYEEGCLSIPEYYAEVERPKTVRIRYTDLDGQVKEEDADGLFATCIQHEIDHLNGVLFVDHISKLKKAMVV
RKFEKAAKRGIKYV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=29.2993630573248, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1789682, Length=166, Percent_Identity=46.9879518072289, Blast_Score=149, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=33.7349397590361, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645726, Length=147, Percent_Identity=31.9727891156463, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_RHOP2 (Q2J2C6)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484295.1
- ProteinModelPortal:   Q2J2C6
- SMR:   Q2J2C6
- STRING:   Q2J2C6
- GeneID:   3908600
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0673
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   MVIKKFT
- ProtClustDB:   PRK00150
- BioCyc:   RPAL316058:RPB_0673-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19741; Mature: 19610

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALREIIILPDKRLREISKPVAEVTPEIRKLADDMFESMYEAPGIGLAAIQIAEPVRLIT
CCCCCEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHH
MDIVRKEGNGKSDPRAFINPEIVGASAELNVYEEGCLSIPEYYAEVERPKTVRIRYTDLD
HHHHHHCCCCCCCCCEECCCEEECCCCCCCHHHHHHCCHHHHHHHCCCCCEEEEEEECCC
GQVKEEDADGLFATCIQHEIDHLNGVLFVDHISKLKKAMVVRKFEKAAKRGIKYV
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALREIIILPDKRLREISKPVAEVTPEIRKLADDMFESMYEAPGIGLAAIQIAEPVRLIT
CCCCEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHH
MDIVRKEGNGKSDPRAFINPEIVGASAELNVYEEGCLSIPEYYAEVERPKTVRIRYTDLD
HHHHHHCCCCCCCCCEECCCEEECCCCCCCHHHHHHCCHHHHHHHCCCCCEEEEEEECCC
GQVKEEDADGLFATCIQHEIDHLNGVLFVDHISKLKKAMVVRKFEKAAKRGIKYV
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA