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The map label for this gene is dapL [H]

Identifier: 86747502

GI number: 86747502

Start: 415696

End: 416958

Strand: Direct

Name: dapL [H]

Synonym: RPB_0376

Alternate gene names: 86747502

Gene position: 415696-416958 (Clockwise)

Preceding gene: 86747501

Following gene: 86747503

Centisome position: 7.8

GC content: 69.83

Gene sequence:

>1263_bases
ATGCGGCCGGGCGCCTTGCAAAACTGGCGTTTTCGAATGGCTGTGCCCGCCTCCTCCGCATCAGCGCACGCTGTCGGCAG
TGCTCACGCTGCCGCGGGCAGCGAGCGCTCGCCATTCGCGCGCCTCACCGAATTGCTGGCGCCGTATCAGCCCGGCGAGC
GGCTGATCAATCTGTCGGTCGGCGAGCCGCAGCATCCGGTGCCGGATTTCGTCGGCCCGGTGCTGGCGCGGCATACCGCC
GAGTTCGGCCGCTATCCGATGGCCAAGGGCATCGCCCCGTTCCGCCAGGCCGCCGCGGCCTGGTTGACGAAACGCTTCGC
GCTGCCGCGCGCGCCCGATCCCGAGACGCAAGTGCTGGTGCTGAACGGCAGCCGCGAGGGCCTGTTCCTCGCCGCGGTCG
CGGCCGCGCGCTATGTCGGCCCGCGCGACGGCACGCCGGCGATCCTGATGCCGAATCCGTTCTATCCGGCCTATGCGGCG
GGCGCCCGCGCGGCGGGCTGCGAGGCGGTGTTCCTGCCGACCAATCCCGCCAACGGCTTCCTGCCCGATCTCGATGCGCT
CGACGCCGCGACGCTGAAGCGCACGGTGGCGATCTACATCGCCTCGCCGGCCAATCCGCAGGGCGCGGTGGCCTCGACTG
GTTACTTCGCGAAGTTGAAGGCGCTGGCGGATCGCCACGGCTTCCTGATCCTCGCCGACGAGTGCTACTCGGAAATCTAC
ACCCGCGATGCGCCCGGCAGCGCGCTGCAGGCGGCCGGCCCCGATTTCCGCAACATCGCGGTGTTCCAGTCGCTGTCGAA
GCGCTCGAACCTTCCGGGCATGCGCGTCGGCTTCGTCGCCGGCGACGCCGATTTCCTCGCGGCCTATCACGAGCTGCGCA
ACGTCGCCGCGCCGCAGGTGCCGGTGCCGCTACAGCACGTCGCGGTCGCCGCCTATGCGGACGAGGCGCATGTCGAGGAG
AACCGGCGGCTGTACCGGCTGAAATTCGATCTCGCCGACCAGATCATCGGCGCGCGCTACGGTTATCAGCGCCCCGCCGG
CGGCTTCTGCCTGTGGCTCGACGTCGCCGCGCAGGGCGGCGACGAAGCCGCGACGATCAATTTGTTCAAGCACGGCGGCG
TCCGCGTCATTCCCGGCAGCTATCTGGCGCGGCCGCAGGCCGACGGCAGCAATCCCGGCGCCGGCTACATTCGCGTCGCG
ATGGTGCAGGACAGTGAGACCACCGCCGAAGCGCTGCACCGTCTGGTGCGGATTCTCGAGTGA

Upstream 100 bases:

>100_bases
CGCACACGCATCGAGGCACGCCGCCGCCGCCCGTCGATGGTTAATCGCGTCTTAACCTCGCCGTATCTATGGTGGCTGAT
CTGTTGTCGCCCGCGCCGGA

Downstream 100 bases:

>100_bases
GTCGCGGGGCTGAAAGTTGAGCATGCCGGCGATCGAACGCGTCATCCCCCTGGTCGGGCACCTGCCGACATCGCTGCGCG
ATGCGCTGGCGCGGCGGCTG

Product: aminotransferase, class I and II

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRPGALQNWRFRMAVPASSASAHAVGSAHAAAGSERSPFARLTELLAPYQPGERLINLSVGEPQHPVPDFVGPVLARHTA
EFGRYPMAKGIAPFRQAAAAWLTKRFALPRAPDPETQVLVLNGSREGLFLAAVAAARYVGPRDGTPAILMPNPFYPAYAA
GARAAGCEAVFLPTNPANGFLPDLDALDAATLKRTVAIYIASPANPQGAVASTGYFAKLKALADRHGFLILADECYSEIY
TRDAPGSALQAAGPDFRNIAVFQSLSKRSNLPGMRVGFVAGDADFLAAYHELRNVAAPQVPVPLQHVAVAAYADEAHVEE
NRRLYRLKFDLADQIIGARYGYQRPAGGFCLWLDVAAQGGDEAATINLFKHGGVRVIPGSYLARPQADGSNPGAGYIRVA
MVQDSETTAEALHRLVRILE

Sequences:

>Translated_420_residues
MRPGALQNWRFRMAVPASSASAHAVGSAHAAAGSERSPFARLTELLAPYQPGERLINLSVGEPQHPVPDFVGPVLARHTA
EFGRYPMAKGIAPFRQAAAAWLTKRFALPRAPDPETQVLVLNGSREGLFLAAVAAARYVGPRDGTPAILMPNPFYPAYAA
GARAAGCEAVFLPTNPANGFLPDLDALDAATLKRTVAIYIASPANPQGAVASTGYFAKLKALADRHGFLILADECYSEIY
TRDAPGSALQAAGPDFRNIAVFQSLSKRSNLPGMRVGFVAGDADFLAAYHELRNVAAPQVPVPLQHVAVAAYADEAHVEE
NRRLYRLKFDLADQIIGARYGYQRPAGGFCLWLDVAAQGGDEAATINLFKHGGVRVIPGSYLARPQADGSNPGAGYIRVA
MVQDSETTAEALHRLVRILE
>Mature_420_residues
MRPGALQNWRFRMAVPASSASAHAVGSAHAAAGSERSPFARLTELLAPYQPGERLINLSVGEPQHPVPDFVGPVLARHTA
EFGRYPMAKGIAPFRQAAAAWLTKRFALPRAPDPETQVLVLNGSREGLFLAAVAAARYVGPRDGTPAILMPNPFYPAYAA
GARAAGCEAVFLPTNPANGFLPDLDALDAATLKRTVAIYIASPANPQGAVASTGYFAKLKALADRHGFLILADECYSEIY
TRDAPGSALQAAGPDFRNIAVFQSLSKRSNLPGMRVGFVAGDADFLAAYHELRNVAAPQVPVPLQHVAVAAYADEAHVEE
NRRLYRLKFDLADQIIGARYGYQRPAGGFCLWLDVAAQGGDEAATINLFKHGGVRVIPGSYLARPQADGSNPGAGYIRVA
MVQDSETTAEALHRLVRILE

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507369, Length=236, Percent_Identity=25.4237288135593, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788722, Length=377, Percent_Identity=26.2599469496021, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788627, Length=389, Percent_Identity=23.1362467866324, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786816, Length=336, Percent_Identity=26.4880952380952, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790797, Length=311, Percent_Identity=25.08038585209, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788332, Length=284, Percent_Identity=27.8169014084507, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17567663, Length=407, Percent_Identity=23.3415233415233, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR019881
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.83 [H]

Molecular weight: Translated: 44818; Mature: 44818

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGALQNWRFRMAVPASSASAHAVGSAHAAAGSERSPFARLTELLAPYQPGERLINLSV
CCCCCCCCCEEEEEECCCCCCCHHCCCHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
GEPQHPVPDFVGPVLARHTAEFGRYPMAKGIAPFRQAAAAWLTKRFALPRAPDPETQVLV
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
LNGSREGLFLAAVAAARYVGPRDGTPAILMPNPFYPAYAAGARAAGCEAVFLPTNPANGF
EECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHCCCCCCCCCEEEECCCCCCCC
LPDLDALDAATLKRTVAIYIASPANPQGAVASTGYFAKLKALADRHGFLILADECYSEIY
CCCCHHHHHHHHEEEEEEEEECCCCCCCCEECCCHHHHHHHHHCCCCEEEEHHHHHHHHH
TRDAPGSALQAAGPDFRNIAVFQSLSKRSNLPGMRVGFVAGDADFLAAYHELRNVAAPQV
CCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
PVPLQHVAVAAYADEAHVEENRRLYRLKFDLADQIIGARYGYQRPAGGFCLWLDVAAQGG
CCCHHHHEEEECCCHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCC
DEAATINLFKHGGVRVIPGSYLARPQADGSNPGAGYIRVAMVQDSETTAEALHRLVRILE
CCEEEEEEEECCCEEEECCHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MRPGALQNWRFRMAVPASSASAHAVGSAHAAAGSERSPFARLTELLAPYQPGERLINLSV
CCCCCCCCCEEEEEECCCCCCCHHCCCHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
GEPQHPVPDFVGPVLARHTAEFGRYPMAKGIAPFRQAAAAWLTKRFALPRAPDPETQVLV
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
LNGSREGLFLAAVAAARYVGPRDGTPAILMPNPFYPAYAAGARAAGCEAVFLPTNPANGF
EECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHCCCCCCCCCEEEECCCCCCCC
LPDLDALDAATLKRTVAIYIASPANPQGAVASTGYFAKLKALADRHGFLILADECYSEIY
CCCCHHHHHHHHEEEEEEEEECCCCCCCCEECCCHHHHHHHHHCCCCEEEEHHHHHHHHH
TRDAPGSALQAAGPDFRNIAVFQSLSKRSNLPGMRVGFVAGDADFLAAYHELRNVAAPQV
CCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
PVPLQHVAVAAYADEAHVEENRRLYRLKFDLADQIIGARYGYQRPAGGFCLWLDVAAQGG
CCCHHHHEEEECCCHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCC
DEAATINLFKHGGVRVIPGSYLARPQADGSNPGAGYIRVAMVQDSETTAEALHRLVRILE
CCEEEEEEEECCCEEEECCHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA