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The map label for this gene is hbpA [H]

Identifier: 86747352

GI number: 86747352

Start: 254956

End: 256557

Strand: Direct

Name: hbpA [H]

Synonym: RPB_0226

Alternate gene names: 86747352

Gene position: 254956-256557 (Clockwise)

Preceding gene: 86747344

Following gene: 86747361

Centisome position: 4.78

GC content: 63.55

Gene sequence:

>1602_bases
ATGAAGCGATTTGGAAGTGCGTTGGTGCTGGCCGCGATGATCGCAGCCGCGGGCGTCATCGGGCCCGCGCGTGCGGAATC
GGTGGTGCGTTACGGCATCTCTATGGCGGACATTCCGCTCACCACCGGCCAGCCCGATCGCGGCGCAGGTGCCTATCAAT
TCACCGGCTATACGATCTACGATCCGCTGGTGGCCTGGGAGATGAATGTCGCCGATCGGCCCGGCAAGCTGGTGCCCGGC
CTTGCGACCGAATGGAAAGTCGACGAGTCCGACAAGACCAAATGGCGCTTTACTCTGCGCAAGGGCGTCAAGTTCCACGA
CGGCAGCGACTTCAATGCCGACGCAGTGATCTGGAATCTCGACAAGGTGCTCAACGAAAAGGCACCGCAGTTCGACAAGC
GGCAGAGCGCGCAGGTCAAGACCCGGCTTCCGTCGGTAAAGAGTTACGCCAAGATCGACGATTCAACCATCGAGATCACC
ACCAAGGCGGTCGACTCCTTCTTCCCCTATCAGATGCTCTGGTTCCTGGTGTCGAGCCCGGCGCAATACGACAAGGTCGG
CAAGGACTGGGACAAGTTCGCGGCGCAGCCGTCGGGCACGGGTCCCTTCAAGCTCACCAAGCTGGTGCCGCGCGAACTCG
CCGAGCTGACCAGGAACGCCGACTATTGGGACAAGGCGCGGCTGCCGAAGACCGACAAGCTCGTGCTGGTGCCGATGCCC
GAAGCGCTCACCCGCACCAACGCGTTGCTCGCCGGCCAGGTCGATCTGATCGAGACGCCCGCGCCCGATGCCGTGCCGCA
GCTCAAATCGGCCGGCATGAAGCTGGTCGACAATGTCACGCCGCATGTCTGGAACTATCACCTCAGCGTGCTGCCCGGCT
CGCCGTGGACCGATGTCCGTCTGCGCAAGGCGCTGAATCTCGCGATCGACCGCGACGCCGTGGTCGGGCTGATGAACGGC
CTCGCCAAGCCGGCGGTCGGCCAGGTCGATCCGTCGAGCCCGTGGTTCGGCAAGCCGACCTTCAAGATCAAATACGACCT
CGCCGAGGCCAAGCGGCTGGTGAAGGAAGCCGGCTACTCGCCGGAGAAGCCGCTGAAGGCCAAGTTCATCATCGCCACCG
GCGGCACCGGCCAGATGCTGTCGCTGCCGATGAACGAGTTCCTGCAGCAGAGCTTCAAGGAGATCGGCATCGACGTCGAG
TTCAAGGTGGTCGAACTCGAAGTGCTGTACACCGCGTGGCGCAAGGGCGCGGCCGACGAGAGCATGGCCGGCATCACCGC
CAACAATATCGCCTATGTGACGTCCGATCCGCTCTACGCGATCGTGCGGTTCTTCCACTCCGGACAGGTGGCGCCGGTCG
GCGTCAATTGGGGCGGCTACAAGAACCCCAAGGTCGACGCGCTGATCGACGAGGCGAAGACCAACTTCGATCCGGTCAAG
CAGGACGAGCTGCTGGCGCAGGCGCATGCCCAGATCGTCGACGACGCGGCGCTGGTCTGGGTGGTGCACGACACCAACCC
GCACGCGCTGTCGCCGAAGGTGAAGAGCTTCGTGCAGGCCCAGCACTGGTTCCAGGACCTGACCACGATCGGGCTGCAAT
AG

Upstream 100 bases:

>100_bases
TCATTCGTATGCATATAATTCGACCGATCGGCCGGCGGACGCCGGACTTGGCACTCGCATTGCAGGCTGATGCGCCATGC
TCACGAGGAGATTCGTTGCA

Downstream 100 bases:

>100_bases
ACCGGTTCATCGCTTGATTGAAGCAACGCGGCATTCTCGCGTGCTGAGTCGTCATCCTGAGGTGCGAGCGAAGCGAGCCT
CGAAGGATGCGGCCAAGGTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Hemin-binding lipoprotein [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKRFGSALVLAAMIAAAGVIGPARAESVVRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAWEMNVADRPGKLVPG
LATEWKVDESDKTKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLNEKAPQFDKRQSAQVKTRLPSVKSYAKIDDSTIEIT
TKAVDSFFPYQMLWFLVSSPAQYDKVGKDWDKFAAQPSGTGPFKLTKLVPRELAELTRNADYWDKARLPKTDKLVLVPMP
EALTRTNALLAGQVDLIETPAPDAVPQLKSAGMKLVDNVTPHVWNYHLSVLPGSPWTDVRLRKALNLAIDRDAVVGLMNG
LAKPAVGQVDPSSPWFGKPTFKIKYDLAEAKRLVKEAGYSPEKPLKAKFIIATGGTGQMLSLPMNEFLQQSFKEIGIDVE
FKVVELEVLYTAWRKGAADESMAGITANNIAYVTSDPLYAIVRFFHSGQVAPVGVNWGGYKNPKVDALIDEAKTNFDPVK
QDELLAQAHAQIVDDAALVWVVHDTNPHALSPKVKSFVQAQHWFQDLTTIGLQ

Sequences:

>Translated_533_residues
MKRFGSALVLAAMIAAAGVIGPARAESVVRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAWEMNVADRPGKLVPG
LATEWKVDESDKTKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLNEKAPQFDKRQSAQVKTRLPSVKSYAKIDDSTIEIT
TKAVDSFFPYQMLWFLVSSPAQYDKVGKDWDKFAAQPSGTGPFKLTKLVPRELAELTRNADYWDKARLPKTDKLVLVPMP
EALTRTNALLAGQVDLIETPAPDAVPQLKSAGMKLVDNVTPHVWNYHLSVLPGSPWTDVRLRKALNLAIDRDAVVGLMNG
LAKPAVGQVDPSSPWFGKPTFKIKYDLAEAKRLVKEAGYSPEKPLKAKFIIATGGTGQMLSLPMNEFLQQSFKEIGIDVE
FKVVELEVLYTAWRKGAADESMAGITANNIAYVTSDPLYAIVRFFHSGQVAPVGVNWGGYKNPKVDALIDEAKTNFDPVK
QDELLAQAHAQIVDDAALVWVVHDTNPHALSPKVKSFVQAQHWFQDLTTIGLQ
>Mature_533_residues
MKRFGSALVLAAMIAAAGVIGPARAESVVRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAWEMNVADRPGKLVPG
LATEWKVDESDKTKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLNEKAPQFDKRQSAQVKTRLPSVKSYAKIDDSTIEIT
TKAVDSFFPYQMLWFLVSSPAQYDKVGKDWDKFAAQPSGTGPFKLTKLVPRELAELTRNADYWDKARLPKTDKLVLVPMP
EALTRTNALLAGQVDLIETPAPDAVPQLKSAGMKLVDNVTPHVWNYHLSVLPGSPWTDVRLRKALNLAIDRDAVVGLMNG
LAKPAVGQVDPSSPWFGKPTFKIKYDLAEAKRLVKEAGYSPEKPLKAKFIIATGGTGQMLSLPMNEFLQQSFKEIGIDVE
FKVVELEVLYTAWRKGAADESMAGITANNIAYVTSDPLYAIVRFFHSGQVAPVGVNWGGYKNPKVDALIDEAKTNFDPVK
QDELLAQAHAQIVDDAALVWVVHDTNPHALSPKVKSFVQAQHWFQDLTTIGLQ

Specific function: Important role in heme acquisition or metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=475, Percent_Identity=25.4736842105263, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789966, Length=557, Percent_Identity=24.9551166965889, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1787052, Length=433, Percent_Identity=27.7136258660508, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1789397, Length=466, Percent_Identity=23.3905579399142, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789887, Length=394, Percent_Identity=25.6345177664975, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081878, Length=480, Percent_Identity=23.3333333333333, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787762, Length=471, Percent_Identity=24.203821656051, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787551, Length=312, Percent_Identity=26.6025641025641, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58798; Mature: 58798

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFGSALVLAAMIAAAGVIGPARAESVVRYGISMADIPLTTGQPDRGAGAYQFTGYTIY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEECCEEEE
DPLVAWEMNVADRPGKLVPGLATEWKVDESDKTKWRFTLRKGVKFHDGSDFNADAVIWNL
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCEEEEEH
DKVLNEKAPQFDKRQSAQVKTRLPSVKSYAKIDDSTIEITTKAVDSFFPYQMLWFLVSSP
HHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHCHHHHHHHHHCCC
AQYDKVGKDWDKFAAQPSGTGPFKLTKLVPRELAELTRNADYWDKARLPKTDKLVLVPMP
HHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEECC
EALTRTNALLAGQVDLIETPAPDAVPQLKSAGMKLVDNVTPHVWNYHLSVLPGSPWTDVR
HHHHHCCCEEECCEEEECCCCCCHHHHHHHCCHHHHHCCCCCEEEEEEEEECCCCCHHHH
LRKALNLAIDRDAVVGLMNGLAKPAVGQVDPSSPWFGKPTFKIKYDLAEAKRLVKEAGYS
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCC
PEKPLKAKFIIATGGTGQMLSLPMNEFLQQSFKEIGIDVEFKVVELEVLYTAWRKGAADE
CCCCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHCCCCCC
SMAGITANNIAYVTSDPLYAIVRFFHSGQVAPVGVNWGGYKNPKVDALIDEAKTNFDPVK
HHCCEEECCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCC
QDELLAQAHAQIVDDAALVWVVHDTNPHALSPKVKSFVQAQHWFQDLTTIGLQ
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRFGSALVLAAMIAAAGVIGPARAESVVRYGISMADIPLTTGQPDRGAGAYQFTGYTIY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEECCEEEE
DPLVAWEMNVADRPGKLVPGLATEWKVDESDKTKWRFTLRKGVKFHDGSDFNADAVIWNL
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCEEEEEH
DKVLNEKAPQFDKRQSAQVKTRLPSVKSYAKIDDSTIEITTKAVDSFFPYQMLWFLVSSP
HHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHCHHHHHHHHHCCC
AQYDKVGKDWDKFAAQPSGTGPFKLTKLVPRELAELTRNADYWDKARLPKTDKLVLVPMP
HHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEECC
EALTRTNALLAGQVDLIETPAPDAVPQLKSAGMKLVDNVTPHVWNYHLSVLPGSPWTDVR
HHHHHCCCEEECCEEEECCCCCCHHHHHHHCCHHHHHCCCCCEEEEEEEEECCCCCHHHH
LRKALNLAIDRDAVVGLMNGLAKPAVGQVDPSSPWFGKPTFKIKYDLAEAKRLVKEAGYS
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCC
PEKPLKAKFIIATGGTGQMLSLPMNEFLQQSFKEIGIDVEFKVVELEVLYTAWRKGAADE
CCCCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHCCCCCC
SMAGITANNIAYVTSDPLYAIVRFFHSGQVAPVGVNWGGYKNPKVDALIDEAKTNFDPVK
HHCCEEECCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCC
QDELLAQAHAQIVDDAALVWVVHDTNPHALSPKVKSFVQAQHWFQDLTTIGLQ
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339409; 7542800; 2041470 [H]