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The map label for this gene is phaG [H]

Identifier: 86747280

GI number: 86747280

Start: 166200

End: 166619

Strand: Direct

Name: phaG [H]

Synonym: RPB_0153

Alternate gene names: 86747280

Gene position: 166200-166619 (Clockwise)

Preceding gene: 86747279

Following gene: 86747284

Centisome position: 3.12

GC content: 63.81

Gene sequence:

>420_bases
ATGACGATGGCCGGACTGAGCGAATTGCTGGTGTCGGCGCTGATCCTGATCGGCGCGTTCTTCCTGTTCGTCGGATCGTT
CGGTCTGGTGAAGCTGCCGGACGTGATGCGCCGGCTGCATGCGCCGACCAAATCCACCACGCTCGGCATCGGCTCGCTGC
TGATCGCCTCGATGCTGTATTTAGCGCTGCTGCGGGACGATCCGTCGCTGCACGAACTGATCATCTCGGTGTTCCTGTTC
CTCACCGCGCCGATCACCGCGCACATGATCTCCAAGGCCCACATCCTGCGCAACCGCGCGCTGCAGCAGGAATTGCCGCA
GCCGCCCGGCGACGAAGGCTGGGCGACGCTCGACCCGCATGGGGCGAACGAGACGCGCCTCGATCCGGAACCGACACGGT
CGACAAACGCCAAAGATTGA

Upstream 100 bases:

>100_bases
AGCCTGTATTTCGAAATCGCGCTTTTGTTCGCGTCGGTCGGGTTCCTGTCGACGGTCGCGTTCTGCAAATATCTGCTGCG
CGGCAACGTGATCGAATAGC

Downstream 100 bases:

>100_bases
AAACACCCGTTTCAGCCCGTCATGGCCGGGCTCGTCCCGGCCATCCACGTCTTGCCTCTTGCACGATCCTCAGACGTGGA
TGCCCGCGACAAGCGCGGGC

Product: putative monovalent cation/H+ antiporter subunit G

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein G; Pha system subunit G [H]

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MTMAGLSELLVSALILIGAFFLFVGSFGLVKLPDVMRRLHAPTKSTTLGIGSLLIASMLYLALLRDDPSLHELIISVFLF
LTAPITAHMISKAHILRNRALQQELPQPPGDEGWATLDPHGANETRLDPEPTRSTNAKD

Sequences:

>Translated_139_residues
MTMAGLSELLVSALILIGAFFLFVGSFGLVKLPDVMRRLHAPTKSTTLGIGSLLIASMLYLALLRDDPSLHELIISVFLF
LTAPITAHMISKAHILRNRALQQELPQPPGDEGWATLDPHGANETRLDPEPTRSTNAKD
>Mature_138_residues
TMAGLSELLVSALILIGAFFLFVGSFGLVKLPDVMRRLHAPTKSTTLGIGSLLIASMLYLALLRDDPSLHELIISVFLFL
TAPITAHMISKAHILRNRALQQELPQPPGDEGWATLDPHGANETRLDPEPTRSTNAKD

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG1320

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhG subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit G family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005133 [H]

Pfam domain/function: PF03334 PhaG_MnhG_YufB [H]

EC number: NA

Molecular weight: Translated: 15139; Mature: 15008

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAGLSELLVSALILIGAFFLFVGSFGLVKLPDVMRRLHAPTKSTTLGIGSLLIASMLY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LALLRDDPSLHELIISVFLFLTAPITAHMISKAHILRNRALQQELPQPPGDEGWATLDPH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCC
GANETRLDPEPTRSTNAKD
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMAGLSELLVSALILIGAFFLFVGSFGLVKLPDVMRRLHAPTKSTTLGIGSLLIASMLY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LALLRDDPSLHELIISVFLFLTAPITAHMISKAHILRNRALQQELPQPPGDEGWATLDPH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCC
GANETRLDPEPTRSTNAKD
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]