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The map label for this gene is phaF [H]

Identifier: 86747279

GI number: 86747279

Start: 165929

End: 166198

Strand: Direct

Name: phaF [H]

Synonym: RPB_0152

Alternate gene names: 86747279

Gene position: 165929-166198 (Clockwise)

Preceding gene: 86747278

Following gene: 86747280

Centisome position: 3.11

GC content: 60.74

Gene sequence:

>270_bases
ATGATCGCAACAGCGTGCCTCATCGCCGTCGGGGTGATCAGCGTCTCGGCGCTGATGAACCTCTTCCGGCTCGCCACCGG
GCCCGACGTGCTCGACCGGATCCTGTCGCTCGATACGCTGACGATCAACGCCATCGGCCTCGTCGTGGTGATCGGAATCT
GGTTCGGCACCAGCCTGTATTTCGAAATCGCGCTTTTGTTCGCGTCGGTCGGGTTCCTGTCGACGGTCGCGTTCTGCAAA
TATCTGCTGCGCGGCAACGTGATCGAATAG

Upstream 100 bases:

>100_bases
GCAGCGCCATTCTGGTGCATTGCCTGCACACCACCGATCCCGTCGACACGGTCGAGCAGATCAAGACTCGCTACGAACGA
CGACTGAAGGAGATCTTCGG

Downstream 100 bases:

>100_bases
CATGACGATGGCCGGACTGAGCGAATTGCTGGTGTCGGCGCTGATCCTGATCGGCGCGTTCTTCCTGTTCGTCGGATCGT
TCGGTCTGGTGAAGCTGCCG

Product: putative monovalent cation/H+ antiporter subunit F

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein F; Pha system subunit F [H]

Number of amino acids: Translated: 89; Mature: 89

Protein sequence:

>89_residues
MIATACLIAVGVISVSALMNLFRLATGPDVLDRILSLDTLTINAIGLVVVIGIWFGTSLYFEIALLFASVGFLSTVAFCK
YLLRGNVIE

Sequences:

>Translated_89_residues
MIATACLIAVGVISVSALMNLFRLATGPDVLDRILSLDTLTINAIGLVVVIGIWFGTSLYFEIALLFASVGFLSTVAFCK
YLLRGNVIE
>Mature_89_residues
MIATACLIAVGVISVSALMNLFRLATGPDVLDRILSLDTLTINAIGLVVVIGIWFGTSLYFEIALLFASVGFLSTVAFCK
YLLRGNVIE

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG2212

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhF subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007208 [H]

Pfam domain/function: PF04066 MrpF_PhaF [H]

EC number: NA

Molecular weight: Translated: 9511; Mature: 9511

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATACLIAVGVISVSALMNLFRLATGPDVLDRILSLDTLTINAIGLVVVIGIWFGTSLY
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEIALLFASVGFLSTVAFCKYLLRGNVIE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIATACLIAVGVISVSALMNLFRLATGPDVLDRILSLDTLTINAIGLVVVIGIWFGTSLY
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEIALLFASVGFLSTVAFCKYLLRGNVIE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]