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The map label for this gene is azoB [H]

Identifier: 86610295

GI number: 86610295

Start: 2970578

End: 2971534

Strand: Direct

Name: azoB [H]

Synonym: CYB_2874

Alternate gene names: 86610295

Gene position: 2970578-2971534 (Clockwise)

Preceding gene: 86610294

Following gene: 86610296

Centisome position: 97.5

GC content: 57.16

Gene sequence:

>957_bases
ATGAACGTGCTGGTTGTGGGGGCGACGGGAACGCTGGGCCGTCAAGTGGTACGCCGAGCCATCGAAGAGGGACACCAAGT
TACCTGTCTGGTGCGAAATCCGGCAAAGGCTGCTTTCTTAAGTGAATGGGGCGCTCACCTGAAGGTGGGAAACCTGCTTC
AGCCCAGCACCCTCAACTCCGCGATGGAAGACATCGAGGCGGTGCTCGACTGTGCTACGGTGCGGGTGACCGACACCCTC
AGCGCCCGTCAGGTGGACTGGGATGGCAAGGTTGCCCTGATCAATGCGGCACGGGCTGCCCAGGTAGGGCATTTTATCTT
TTTCTCCATCATGGGCGCCCACCACGAGTATCCCAATGTGCCCCTGATGAATTTCAAGCATCACATTGAGAAGTATTTGA
TCGGATCCCAGATGCCCTACACCATTTTCCGGCCAGCGGGCTTTATGCAGGGGTTGATTGGCCAGTACGCCATCCCCATC
TTGGAAGAACAAATCGTCTGGGTAACCGGCGAGGGCATGCCAACGGCCTACCTAGACACCCTAGATGCAGCCCGGTTTGC
GGTGCGAGCTCTCACTGTAGAAGCTGCCAAGCAGCAAATCTTTCCCTTGGTGGGGCCCAAGGCTTGGACGGCTCGAGAAG
TCATTGCCTTGTGCGAGCAGCTCTCCGGCAAAAAGGCCAAAGTCAGCACCATGCCGCTGGGACTGCTGAGAGGGGCGCGC
AAGGTGGCGCAGTTTTTTCAGTGGAGCTGGAACATTGCCGACCGGCTGGCGTTTGTGGAGGTGATCGCGGCCAAAGAACC
CATGCACGCCGATATGACGCCTGTATGCGAAACCCTGGGGATTGAACTGGCCTCTCTGACCTCTCTTGAGGACTACCTGG
CGGAGTATTTCCAGAAGATGTTGAAACGGATTCGCGAGCTGAAGGTCAAGCAACCTAAAGTCAAAACGCCCTTCTAG

Upstream 100 bases:

>100_bases
TTGGCAGAGGATCCCCATTGGTTTGTTGCTGCCTCTAAGGTTGTGTCGGGAGTTGCGCTGGTCTGATAGACTAAGCCTAA
GGTTAAGAAATGATAATTTT

Downstream 100 bases:

>100_bases
GTCGTTGTTCTCAATCTTCATCTTCTTGGCAATTTCACAGGGACTTACGATGGTAGCCTTGGGATGAAGAGGTGAATGGG
ATCCTGCTTGCGTTAGCCTA

Product: NAD-dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNVLVVGATGTLGRQVVRRAIEEGHQVTCLVRNPAKAAFLSEWGAHLKVGNLLQPSTLNSAMEDIEAVLDCATVRVTDTL
SARQVDWDGKVALINAARAAQVGHFIFFSIMGAHHEYPNVPLMNFKHHIEKYLIGSQMPYTIFRPAGFMQGLIGQYAIPI
LEEQIVWVTGEGMPTAYLDTLDAARFAVRALTVEAAKQQIFPLVGPKAWTAREVIALCEQLSGKKAKVSTMPLGLLRGAR
KVAQFFQWSWNIADRLAFVEVIAAKEPMHADMTPVCETLGIELASLTSLEDYLAEYFQKMLKRIRELKVKQPKVKTPF

Sequences:

>Translated_318_residues
MNVLVVGATGTLGRQVVRRAIEEGHQVTCLVRNPAKAAFLSEWGAHLKVGNLLQPSTLNSAMEDIEAVLDCATVRVTDTL
SARQVDWDGKVALINAARAAQVGHFIFFSIMGAHHEYPNVPLMNFKHHIEKYLIGSQMPYTIFRPAGFMQGLIGQYAIPI
LEEQIVWVTGEGMPTAYLDTLDAARFAVRALTVEAAKQQIFPLVGPKAWTAREVIALCEQLSGKKAKVSTMPLGLLRGAR
KVAQFFQWSWNIADRLAFVEVIAAKEPMHADMTPVCETLGIELASLTSLEDYLAEYFQKMLKRIRELKVKQPKVKTPF
>Mature_318_residues
MNVLVVGATGTLGRQVVRRAIEEGHQVTCLVRNPAKAAFLSEWGAHLKVGNLLQPSTLNSAMEDIEAVLDCATVRVTDTL
SARQVDWDGKVALINAARAAQVGHFIFFSIMGAHHEYPNVPLMNFKHHIEKYLIGSQMPYTIFRPAGFMQGLIGQYAIPI
LEEQIVWVTGEGMPTAYLDTLDAARFAVRALTVEAAKQQIFPLVGPKAWTAREVIALCEQLSGKKAKVSTMPLGLLRGAR
KVAQFFQWSWNIADRLAFVEVIAAKEPMHADMTPVCETLGIELASLTSLEDYLAEYFQKMLKRIRELKVKQPKVKTPF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=282, Percent_Identity=24.822695035461, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 35304; Mature: 35304

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVVGATGTLGRQVVRRAIEEGHQVTCLVRNPAKAAFLSEWGAHLKVGNLLQPSTLNS
CCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCEEECCCCCCHHHHH
AMEDIEAVLDCATVRVTDTLSARQVDWDGKVALINAARAAQVGHFIFFSIMGAHHEYPNV
HHHHHHHHHHHHHEEEECCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCC
PLMNFKHHIEKYLIGSQMPYTIFRPAGFMQGLIGQYAIPILEEQIVWVTGEGMPTAYLDT
CHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
LDAARFAVRALTVEAAKQQIFPLVGPKAWTAREVIALCEQLSGKKAKVSTMPLGLLRGAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEHHCCHHHHHHHH
KVAQFFQWSWNIADRLAFVEVIAAKEPMHADMTPVCETLGIELASLTSLEDYLAEYFQKM
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LKRIRELKVKQPKVKTPF
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVLVVGATGTLGRQVVRRAIEEGHQVTCLVRNPAKAAFLSEWGAHLKVGNLLQPSTLNS
CCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCEEECCCCCCHHHHH
AMEDIEAVLDCATVRVTDTLSARQVDWDGKVALINAARAAQVGHFIFFSIMGAHHEYPNV
HHHHHHHHHHHHHEEEECCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCC
PLMNFKHHIEKYLIGSQMPYTIFRPAGFMQGLIGQYAIPILEEQIVWVTGEGMPTAYLDT
CHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
LDAARFAVRALTVEAAKQQIFPLVGPKAWTAREVIALCEQLSGKKAKVSTMPLGLLRGAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEHHCCHHHHHHHH
KVAQFFQWSWNIADRLAFVEVIAAKEPMHADMTPVCETLGIELASLTSLEDYLAEYFQKM
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LKRIRELKVKQPKVKTPF
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]