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The map label for this gene is ndhF [H]

Identifier: 86610296

GI number: 86610296

Start: 2971940

End: 2973775

Strand: Direct

Name: ndhF [H]

Synonym: CYB_2875

Alternate gene names: 86610296

Gene position: 2971940-2973775 (Clockwise)

Preceding gene: 86610295

Following gene: 86610297

Centisome position: 97.55

GC content: 60.29

Gene sequence:

>1836_bases
ATGAGCTTGAACGGGCTGGTCACAACTGCATGGCTGATTCCCCTCTACGCTTTGGTGGCGTGGGCGGGAGCGCTGATCTG
GTCCCCAGGAATCACCCGGCGCACGGGACCACGTCCGGCAGGTTATTTGAATGCCCTGCTGACCTTAGTGGCTTTCCTTC
ACAGTGTGGGAGCGCTGGTGGCGATTTGGGATCAGCCGGCGCAGGTCATTCGGTTGCCTTGGCTGCAGGTGGGGGATCTA
ATGTTAGAGCTGCCGCTGCAGGTGTCATCGGTAACTCTGGGGGCCTGCGCTGTGGTGACGGGTCTGAACCTGGTGGCCCA
GATCTACGGCTTTGGCTACATGGAAATGGACTGGGGCTGGGCGCGGTTTTTCTCCCTGCTGGGCCTGTTTGAGGGGGGGA
TGTGTGCCTTGGCTTTGTGCGACTCCCTCTTTTTCAGCTACGTGATCCTGGAGATTTTGACCCTGGGCACTTACCTGTTG
GTGGGCTTTTGGTTCAACCAATCGCTGGTGGTGACGGGAGCGCGGGATGCTTTTTTAACCAAGCGCATCGGCGACCTGTT
GCTCTTGATGGGGGTAATCGCCATCTATCCCCTGACCGGCTCCTGGGAGTTTGAGGATTTGGCGGCTTGGGCTGAGGCGG
TGCAGGCAGGATCTGTCACTGTGGATCCCACCCTGCTGGCCCTGGTAGGGCTGGGGTTGCTGGCCGGCCCCGTAGGCAAG
TGTGCCCAATTTCCTCTGCACCTGTGGCTGGATGAGGCGATGGAAGGGCCGATCCCCTCGACGATCTTGCGCAATGCGGT
GGTGGTGGGCACAGGGGCCTGGGTGTTGATTCGCCTGCAACCCCTCTACGAGCTTTCCCCAACGGTGCTGAAGGTGATGC
TGACTATCGGCAGCTTGACGGCGGTGGGGGGATCCCTAGTGGCCTTGGCCCAGATCGATGCCAAGCGGGCCCTGTCTTAT
CTGGTGAGCGCCTACATGGGCTTGATCTTTGTGGCGGTGGGCACCGGTCATCCCCAGCAAGCCTATGGGTTGCTGCTGAC
CTATGCGCCCGCGATGGCCCTGTTGGTGATGGCGCTGGGCATGGTGATCTGGAACAGCATTACCCAGGATCTGCGCTATC
TAGGAGGCTTGTGGTCGCGGCGCCCTATCTCGGGGCTGTGCTTCTTGACCGGGATGGCTGGAATCATCGCTTTGCCTCCC
TTCGGCTCCTTTTGGGTGCTGACGGATCTCTGGCAGCAGTTGGTGCAACCGGGGCAGGGGTTGTGGGCGGCAGTTGTGCT
GGTTACCAATGCGGTGACGGCGCTGGGGCTAACGCGGGTATTTGGCCTTGTTTGGGGGGGCAAACCGCAGCAGATGAGCG
AGCGCTCGCCAGAGGTGCATTGGCCGATGATGTTGCCCAGCACCGCCATGATGGGGGTAACGCTGCACATGCCCATGATC
CTATCGGCACTAGGTCTGATTCCCGCTCTGGAAGGGATCCCTGTTGGGGTAATGACGGGCTCCAGCTTGACAGGCATTGC
CTTGGGAGCCTGGCTGTATCTGCGTCCGCAGCGGCAGCCGGTGGACTGGGCCTTCTTGGCCGACTGGCAGGATTTGTTGG
CCAACGACTTCTACACCCCCAAGCTCTACCGGGCCAGTGTGGTGTTTGCGGTACGCATCATCTCTGAGGTTACAGCCTTG
TTCGACCGCTATGTGGTAGACGGCTTGGTAAATTTGACCAGCTTGGCGGTATTGGCGGGTGGACAGGTTTTGAAATACAC
CACCTCTGGCCAGTTGCAAGCCTACGTGCTGACGATTGTGGTGGGGGTGGCCCTGCTGGGCTGGCTGATTCTCTAG

Upstream 100 bases:

>100_bases
TTTATCTATCCTAATTTGCAGCTAAGATGCTGCTTAAGATTTTTTACAGGATCTCAGCAAGTGTTCCAGTTTGGCCAAGA
TCCCTGGCCGAAGGAGGATT

Downstream 100 bases:

>100_bases
GCTTGGGCAAGGTAGCGGGACGGAGTCCTTAGATAAAGAGGGAAAAGACCATGCTGAGTGCCTTGATTGTGTTGCCGATC
TTGGGATCCCTGCTCCTGTT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSLNGLVTTAWLIPLYALVAWAGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALVAIWDQPAQVIRLPWLQVGDL
MLELPLQVSSVTLGACAVVTGLNLVAQIYGFGYMEMDWGWARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLL
VGFWFNQSLVVTGARDAFLTKRIGDLLLLMGVIAIYPLTGSWEFEDLAAWAEAVQAGSVTVDPTLLALVGLGLLAGPVGK
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLKVMLTIGSLTAVGGSLVALAQIDAKRALSY
LVSAYMGLIFVAVGTGHPQQAYGLLLTYAPAMALLVMALGMVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGIIALPP
FGSFWVLTDLWQQLVQPGQGLWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHMPMI
LSALGLIPALEGIPVGVMTGSSLTGIALGAWLYLRPQRQPVDWAFLADWQDLLANDFYTPKLYRASVVFAVRIISEVTAL
FDRYVVDGLVNLTSLAVLAGGQVLKYTTSGQLQAYVLTIVVGVALLGWLIL

Sequences:

>Translated_611_residues
MSLNGLVTTAWLIPLYALVAWAGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALVAIWDQPAQVIRLPWLQVGDL
MLELPLQVSSVTLGACAVVTGLNLVAQIYGFGYMEMDWGWARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLL
VGFWFNQSLVVTGARDAFLTKRIGDLLLLMGVIAIYPLTGSWEFEDLAAWAEAVQAGSVTVDPTLLALVGLGLLAGPVGK
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLKVMLTIGSLTAVGGSLVALAQIDAKRALSY
LVSAYMGLIFVAVGTGHPQQAYGLLLTYAPAMALLVMALGMVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGIIALPP
FGSFWVLTDLWQQLVQPGQGLWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHMPMI
LSALGLIPALEGIPVGVMTGSSLTGIALGAWLYLRPQRQPVDWAFLADWQDLLANDFYTPKLYRASVVFAVRIISEVTAL
FDRYVVDGLVNLTSLAVLAGGQVLKYTTSGQLQAYVLTIVVGVALLGWLIL
>Mature_610_residues
SLNGLVTTAWLIPLYALVAWAGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALVAIWDQPAQVIRLPWLQVGDLM
LELPLQVSSVTLGACAVVTGLNLVAQIYGFGYMEMDWGWARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLLV
GFWFNQSLVVTGARDAFLTKRIGDLLLLMGVIAIYPLTGSWEFEDLAAWAEAVQAGSVTVDPTLLALVGLGLLAGPVGKC
AQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLKVMLTIGSLTAVGGSLVALAQIDAKRALSYL
VSAYMGLIFVAVGTGHPQQAYGLLLTYAPAMALLVMALGMVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGIIALPPF
GSFWVLTDLWQQLVQPGQGLWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHMPMIL
SALGLIPALEGIPVGVMTGSSLTGIALGAWLYLRPQRQPVDWAFLADWQDLLANDFYTPKLYRASVVFAVRIISEVTALF
DRYVVDGLVNLTSLAVLAGGQVLKYTTSGQLQAYVLTIVVGVALLGWLIL

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=359, Percent_Identity=28.9693593314763, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1788614, Length=560, Percent_Identity=28.5714285714286, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788831, Length=394, Percent_Identity=26.3959390862944, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788829, Length=454, Percent_Identity=26.2114537444934, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788827, Length=410, Percent_Identity=25.3658536585366, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788613, Length=302, Percent_Identity=23.841059602649, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 65780; Mature: 65649

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNGLVTTAWLIPLYALVAWAGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEE
AIWDQPAQVIRLPWLQVGDLMLELPLQVSSVTLGACAVVTGLNLVAQIYGFGYMEMDWGW
EEECCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHH
ARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLLVGFWFNQSLVVTGARDAFLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHH
KRIGDLLLLMGVIAIYPLTGSWEFEDLAAWAEAVQAGSVTVDPTLLALVGLGLLAGPVGK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCHHH
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLKVMLTIGSLT
HHHCHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEEECHHHHCCHHHHHHHHHHHHHH
AVGGSLVALAQIDAKRALSYLVSAYMGLIFVAVGTGHPQQAYGLLLTYAPAMALLVMALG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGIIALPPFGSFWVLTDLWQQLVQPGQG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCC
LWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHMPMI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHH
LSALGLIPALEGIPVGVMTGSSLTGIALGAWLYLRPQRQPVDWAFLADWQDLLANDFYTP
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
KLYRASVVFAVRIISEVTALFDRYVVDGLVNLTSLAVLAGGQVLKYTTSGQLQAYVLTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
VGVALLGWLIL
HHHHHHHHHHC
>Mature Secondary Structure 
SLNGLVTTAWLIPLYALVAWAGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEE
AIWDQPAQVIRLPWLQVGDLMLELPLQVSSVTLGACAVVTGLNLVAQIYGFGYMEMDWGW
EEECCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHH
ARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLLVGFWFNQSLVVTGARDAFLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHH
KRIGDLLLLMGVIAIYPLTGSWEFEDLAAWAEAVQAGSVTVDPTLLALVGLGLLAGPVGK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCHHH
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLKVMLTIGSLT
HHHCHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEEECHHHHCCHHHHHHHHHHHHHH
AVGGSLVALAQIDAKRALSYLVSAYMGLIFVAVGTGHPQQAYGLLLTYAPAMALLVMALG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGIIALPPFGSFWVLTDLWQQLVQPGQG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCC
LWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHMPMI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHH
LSALGLIPALEGIPVGVMTGSSLTGIALGAWLYLRPQRQPVDWAFLADWQDLLANDFYTP
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
KLYRASVVFAVRIISEVTALFDRYVVDGLVNLTSLAVLAGGQVLKYTTSGQLQAYVLTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
VGVALLGWLIL
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]