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The map label for this gene is glyS [H]

Identifier: 86609951

GI number: 86609951

Start: 2613613

End: 2615805

Strand: Direct

Name: glyS [H]

Synonym: CYB_2517

Alternate gene names: 86609951

Gene position: 2613613-2615805 (Clockwise)

Preceding gene: 86609950

Following gene: 86609954

Centisome position: 85.79

GC content: 62.65

Gene sequence:

>2193_bases
ATGGTTGCCTATTTGCTAGAAGTCGGCTGTGAAGAGCTCCCAGCCAGTTTTGTGGATTCGGCTCTGGAGCAGTGGCGAAC
CGGGATCCCGGCCTCTTTGGCGGAGGCCCATTTGCAAGCGGAGCAGATGCAGCTTTTCGGTACGCCGCGCCGCCTGGCGG
TTCTGTTGCAGGGGTTGCCCACCCAGCAGCCGGATCGCACTTTGGAGGTGAAGGGGCCGCCGGTTCAGGTCGCCTACCAA
GATGGCCAGCTCACCCCTGCCGGGGAAAAATTTGCCCAAAAACAAGGGGTGGATCCCGGCAGCCTGGAAATCCGGCAAGT
GGGCAAGGGATCCTTTGTGTTTGCGGTGCAGCGGGTGCGGGGCCGACCGACGGCAGCCGTGCTCCAAGAATTGGCACCCG
CCTGGATTACCAACTTGAGTGGGGAACGGCTGATGCGCTGGGCCTGCGGGGATCTGAAGTTTCCGCGCCCCATTCGCTGG
CTGGTTTCCCTTTGGGATGATCAGGTGCTGCCTTTGCTGCTGCCGACCCACGAGGGCAATACCATGCCGGGCTTGGTGGC
CGGGCGGGTTAGCCGGGGGCACCGCGTTTTGGGATCCCAGCAGGTCACTCTGGAGCGGGCCGAGAGCTACGTTCAGCAAA
TGCAAGAGGCCGGGGTCAGCCCCGATCCGGCAGTGCGGGAAGCGTACATTCAGGCCGAGGTAACCAAGCTGGCCAGGCAG
GTGAATGGGGAAGCGCAGATCCCTGCCCCTCTGCTGCGGGAGGTGGTGCAGTTGGTGGAGTGGCCGACGGCGGTTTTGGG
GCGTTTTGAGCCGGAATTTTTGCGCCTGCCGCCGGCGGTGATTGAGACGGTGATGATCATCCACCAACGCTATTTCCCGG
TACGGGATGGCCGGGATCCCCAGAAATTGCTGCCCTATTTCATCACCATTTCCAATGGCGACCCTGGGCAATCGGAGCGG
ATCGGGGCAGGCAACAGCCGGGTGGTACGGGCGCGCCTGTCGGATGCCCGCTTTTTCTACGAAGAGGATCGGCGGATCCC
CTTGGCGCAGCGGGTGGAACGCCTGCAGGCGGTTACTTTTGCCGAAGGGCTGGGATCCATGCGGGACAAGGTGGAGCGCA
TCCGCCACATCAGCCGCCTCATTGCTGCTGCCTTGAACTTGGATGCCCAGGAACAAGCGCTGGTGGAGCGCACGGCTTAC
CTGTGCAAGGCGGATCTGGTTACCCAAATGGTGTACGAATTCCCCGAGCTGCAGGGGATCATGGGGGCCGACTACGCTCG
GCAGGATGGGGAACCGGAGGCGGTAGCCGAGGGGATTGAGCAACACTACTGGCCTTTGGGAGCGGGGGACGCTTTGCCCA
CAGCACTGACGGGGCGGGTGGTGGGCTGGGCAGATCGCCTGGATACCTTGGTGGGCATGTTTCGCTTGGGGCGGATCCCC
ACCGGCTCCTCGGATCGCTTTGCCCTGCGGCGGGCCGCCAACAGCCTGGTGCTGGTTGCCTGGGATGCCGGCTGGACTTT
TGACTTCAACTTGCTGTTGCAGAGCGTGGTGCAGGACTATGCCTTTGGCGATGTCCACACCTTGCGAGCCTTGCAGGAGT
TTTTGGCCCAACGGATACAGACCCTTTTGCAGGAGGAAAAGCGGATTGATTACGACTTGGTCAGGGCCGTAGTGGGGGAT
CCAGAGTCCGTCAAAACCGAGGATCCCCAGAAGGCCGAGCTCACCATGCGGGCCCTGGGCAACCTGCCCTTGACTTTGCT
GCGGGCGGAGTACCTGCAACACCTGCGGGCCACCGGGGAATTGGCGGATCTCTATCCCACCCTCAATCGCTGTGCCCGCC
TAGCAGCCCAAGGGGACTTGGACTACGCCGTGGTGGATCCGGCAGCCGCCGTGGATCCCGACCAGTTGCAGGAGGCGGCA
GAAAGGGAGTTGTACGAGGCTTGCCAGAAGGTCTATCGCCTGGGGGTGGAGCCAGCCCTTCAGGGAGACTTTGTCCCCTT
GGTGGAAGCTCTGCAAGCAGCAGCCCCTCAGGTAGCTCAGTTTTTTGAAGCCGTTCTGGTGATGGATCCGGATCCTGGTC
GGCGGGCCAGCCGGCTCAATTTGCTGGGGGTATTGCGCAACCAGTCGCGCCTGTTGGGGGACATGGGGGCTGTGGTCATG
GCAGGGGAGTCGTCGCCGGAGAAGACCTCCTAG

Upstream 100 bases:

>100_bases
TACCCCAGGTTCGGAGCAGTCGTGAGCCAGAAGGGAGGCCCAGAGCCATAGACTATGTTGATTGAGGAAGTGGGGATAGT
GCCCTGCGGAGCACAGGAGT

Downstream 100 bases:

>100_bases
CTGGCCAATCCAGACACGCTTGGAACCCTCACTCCCGGCCCCTCGGGAGCTTGGGAATGGACATGCAACCTATTATCCAA
GGAAGCGCAGACATTGCCGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MVAYLLEVGCEELPASFVDSALEQWRTGIPASLAEAHLQAEQMQLFGTPRRLAVLLQGLPTQQPDRTLEVKGPPVQVAYQ
DGQLTPAGEKFAQKQGVDPGSLEIRQVGKGSFVFAVQRVRGRPTAAVLQELAPAWITNLSGERLMRWACGDLKFPRPIRW
LVSLWDDQVLPLLLPTHEGNTMPGLVAGRVSRGHRVLGSQQVTLERAESYVQQMQEAGVSPDPAVREAYIQAEVTKLARQ
VNGEAQIPAPLLREVVQLVEWPTAVLGRFEPEFLRLPPAVIETVMIIHQRYFPVRDGRDPQKLLPYFITISNGDPGQSER
IGAGNSRVVRARLSDARFFYEEDRRIPLAQRVERLQAVTFAEGLGSMRDKVERIRHISRLIAAALNLDAQEQALVERTAY
LCKADLVTQMVYEFPELQGIMGADYARQDGEPEAVAEGIEQHYWPLGAGDALPTALTGRVVGWADRLDTLVGMFRLGRIP
TGSSDRFALRRAANSLVLVAWDAGWTFDFNLLLQSVVQDYAFGDVHTLRALQEFLAQRIQTLLQEEKRIDYDLVRAVVGD
PESVKTEDPQKAELTMRALGNLPLTLLRAEYLQHLRATGELADLYPTLNRCARLAAQGDLDYAVVDPAAAVDPDQLQEAA
ERELYEACQKVYRLGVEPALQGDFVPLVEALQAAAPQVAQFFEAVLVMDPDPGRRASRLNLLGVLRNQSRLLGDMGAVVM
AGESSPEKTS

Sequences:

>Translated_730_residues
MVAYLLEVGCEELPASFVDSALEQWRTGIPASLAEAHLQAEQMQLFGTPRRLAVLLQGLPTQQPDRTLEVKGPPVQVAYQ
DGQLTPAGEKFAQKQGVDPGSLEIRQVGKGSFVFAVQRVRGRPTAAVLQELAPAWITNLSGERLMRWACGDLKFPRPIRW
LVSLWDDQVLPLLLPTHEGNTMPGLVAGRVSRGHRVLGSQQVTLERAESYVQQMQEAGVSPDPAVREAYIQAEVTKLARQ
VNGEAQIPAPLLREVVQLVEWPTAVLGRFEPEFLRLPPAVIETVMIIHQRYFPVRDGRDPQKLLPYFITISNGDPGQSER
IGAGNSRVVRARLSDARFFYEEDRRIPLAQRVERLQAVTFAEGLGSMRDKVERIRHISRLIAAALNLDAQEQALVERTAY
LCKADLVTQMVYEFPELQGIMGADYARQDGEPEAVAEGIEQHYWPLGAGDALPTALTGRVVGWADRLDTLVGMFRLGRIP
TGSSDRFALRRAANSLVLVAWDAGWTFDFNLLLQSVVQDYAFGDVHTLRALQEFLAQRIQTLLQEEKRIDYDLVRAVVGD
PESVKTEDPQKAELTMRALGNLPLTLLRAEYLQHLRATGELADLYPTLNRCARLAAQGDLDYAVVDPAAAVDPDQLQEAA
ERELYEACQKVYRLGVEPALQGDFVPLVEALQAAAPQVAQFFEAVLVMDPDPGRRASRLNLLGVLRNQSRLLGDMGAVVM
AGESSPEKTS
>Mature_730_residues
MVAYLLEVGCEELPASFVDSALEQWRTGIPASLAEAHLQAEQMQLFGTPRRLAVLLQGLPTQQPDRTLEVKGPPVQVAYQ
DGQLTPAGEKFAQKQGVDPGSLEIRQVGKGSFVFAVQRVRGRPTAAVLQELAPAWITNLSGERLMRWACGDLKFPRPIRW
LVSLWDDQVLPLLLPTHEGNTMPGLVAGRVSRGHRVLGSQQVTLERAESYVQQMQEAGVSPDPAVREAYIQAEVTKLARQ
VNGEAQIPAPLLREVVQLVEWPTAVLGRFEPEFLRLPPAVIETVMIIHQRYFPVRDGRDPQKLLPYFITISNGDPGQSER
IGAGNSRVVRARLSDARFFYEEDRRIPLAQRVERLQAVTFAEGLGSMRDKVERIRHISRLIAAALNLDAQEQALVERTAY
LCKADLVTQMVYEFPELQGIMGADYARQDGEPEAVAEGIEQHYWPLGAGDALPTALTGRVVGWADRLDTLVGMFRLGRIP
TGSSDRFALRRAANSLVLVAWDAGWTFDFNLLLQSVVQDYAFGDVHTLRALQEFLAQRIQTLLQEEKRIDYDLVRAVVGD
PESVKTEDPQKAELTMRALGNLPLTLLRAEYLQHLRATGELADLYPTLNRCARLAAQGDLDYAVVDPAAAVDPDQLQEAA
ERELYEACQKVYRLGVEPALQGDFVPLVEALQAAAPQVAQFFEAVLVMDPDPGRRASRLNLLGVLRNQSRLLGDMGAVVM
AGESSPEKTS

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=712, Percent_Identity=33.2865168539326, Blast_Score=333, Evalue=3e-92,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80873; Mature: 80873

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAYLLEVGCEELPASFVDSALEQWRTGIPASLAEAHLQAEQMQLFGTPRRLAVLLQGLP
CCHHHHHHCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
TQQPDRTLEVKGPPVQVAYQDGQLTPAGEKFAQKQGVDPGSLEIRQVGKGSFVFAVQRVR
CCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHCCCCCCCEEHHHHHC
GRPTAAVLQELAPAWITNLSGERLMRWACGDLKFPRPIRWLVSLWDDQVLPLLLPTHEGN
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCC
TMPGLVAGRVSRGHRVLGSQQVTLERAESYVQQMQEAGVSPDPAVREAYIQAEVTKLARQ
CCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VNGEAQIPAPLLREVVQLVEWPTAVLGRFEPEFLRLPPAVIETVMIIHQRYFPVRDGRDP
HCCCCCCCHHHHHHHHHHHHCHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCH
QKLLPYFITISNGDPGQSERIGAGNSRVVRARLSDARFFYEEDRRIPLAQRVERLQAVTF
HHHCCEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AEGLGSMRDKVERIRHISRLIAAALNLDAQEQALVERTAYLCKADLVTQMVYEFPELQGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MGADYARQDGEPEAVAEGIEQHYWPLGAGDALPTALTGRVVGWADRLDTLVGMFRLGRIP
CCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGSSDRFALRRAANSLVLVAWDAGWTFDFNLLLQSVVQDYAFGDVHTLRALQEFLAQRIQ
CCCCCHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLLQEEKRIDYDLVRAVVGDPESVKTEDPQKAELTMRALGNLPLTLLRAEYLQHLRATGE
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCH
LADLYPTLNRCARLAAQGDLDYAVVDPAAAVDPDQLQEAAERELYEACQKVYRLGVEPAL
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QGDFVPLVEALQAAAPQVAQFFEAVLVMDPDPGRRASRLNLLGVLRNQSRLLGDMGAVVM
CCCHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
AGESSPEKTS
ECCCCCCCCC
>Mature Secondary Structure
MVAYLLEVGCEELPASFVDSALEQWRTGIPASLAEAHLQAEQMQLFGTPRRLAVLLQGLP
CCHHHHHHCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
TQQPDRTLEVKGPPVQVAYQDGQLTPAGEKFAQKQGVDPGSLEIRQVGKGSFVFAVQRVR
CCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHCCCCCCCEEHHHHHC
GRPTAAVLQELAPAWITNLSGERLMRWACGDLKFPRPIRWLVSLWDDQVLPLLLPTHEGN
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCC
TMPGLVAGRVSRGHRVLGSQQVTLERAESYVQQMQEAGVSPDPAVREAYIQAEVTKLARQ
CCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VNGEAQIPAPLLREVVQLVEWPTAVLGRFEPEFLRLPPAVIETVMIIHQRYFPVRDGRDP
HCCCCCCCHHHHHHHHHHHHCHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCH
QKLLPYFITISNGDPGQSERIGAGNSRVVRARLSDARFFYEEDRRIPLAQRVERLQAVTF
HHHCCEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AEGLGSMRDKVERIRHISRLIAAALNLDAQEQALVERTAYLCKADLVTQMVYEFPELQGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MGADYARQDGEPEAVAEGIEQHYWPLGAGDALPTALTGRVVGWADRLDTLVGMFRLGRIP
CCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGSSDRFALRRAANSLVLVAWDAGWTFDFNLLLQSVVQDYAFGDVHTLRALQEFLAQRIQ
CCCCCHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLLQEEKRIDYDLVRAVVGDPESVKTEDPQKAELTMRALGNLPLTLLRAEYLQHLRATGE
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCH
LADLYPTLNRCARLAAQGDLDYAVVDPAAAVDPDQLQEAAERELYEACQKVYRLGVEPAL
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QGDFVPLVEALQAAAPQVAQFFEAVLVMDPDPGRRASRLNLLGVLRNQSRLLGDMGAVVM
CCCHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
AGESSPEKTS
ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA