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The map label for this gene is pcrA [H]

Identifier: 86609547

GI number: 86609547

Start: 2194342

End: 2196501

Strand: Reverse

Name: pcrA [H]

Synonym: CYB_2100

Alternate gene names: 86609547

Gene position: 2196501-2194342 (Counterclockwise)

Preceding gene: 86609548

Following gene: 86609546

Centisome position: 72.09

GC content: 61.76

Gene sequence:

>2160_bases
ATGCTGGGTCTGGCTTCTTCTCCCTTTCCTGAGGTCACCCTGCGTCCGGCGCAGCAACAGGCGTTGGCCTACCAATCGGG
GCCGCTGGGGATCGCCGCCGTTCCGGGGAGCGGCAAGACCTTTATTCTGGAGCTGTTGATCGCCGATTTAATTCTCAATC
GAGGGATCCCACCGGAGCGCATCGGGGTGTTCACCTACATGCGTTCGGCGCGGGGCAACTTGATCAGCCGCATCAATCAA
CGTTTGCAACGGGCGGGGTATCCGGATCGCTTTACCCAGGCCTTTACCCTGCATTCTTTGTCGTTGCGGGTGTTGCGGGA
GGCCCCCAGCCGTTGGGAGGAGCTGTCTATTTTGGAGGGATACGAACAGGAGCGGTTGCTCAGCCGCCTGTGCCGCGCTT
GGCTCGTCCGCCATACTCAGCTCTGGGAGCCGCTGATCCCCGGCGACAACAACCCGCAAAAGATCGCCCAAAACCGAGCC
CAGTTCCGGCGCAGTTTCCAGCAGATGGTGCAGGTGGTCATCAGCAGGGCCAAGAATCTGCGCCTGGGGCCAGAAGTGAT
CCCCAGCGAGGGCTATCTGGGCTGGGCTGCTCCCATCTACGCCGGTTATCAAGCGGAATTGCGTCGCCTGGGCAAGTTAG
ACTACGACGACCTGGGCTGGCAAGCAGTGGATTTGATCGAGGGCAGTCCAGAGCTGCGGGAGCAGGTGCAGAACTGGTAC
GACTACCTGTTCGAGGATGAGGCCCAAGACAGTTCCCCCCTGCAGGAAGAGCTGCTGCGGCTGCTCAGCCAACGCACCGG
CAACCTGGTGCGGGTGGGGGATCCCAACCAGAGCATCATGGGCACCTTTACCAGCGCCGAGCCGCGGCTGTTTCGCGCCT
TTTGCCAGGCCAGTCCTCAGGTGGTGCTTTTGGATGAATCCAGCCGCAGTGCCCCCCAGATCATCGCTTTGGCCAACCGC
CTGGTGGAGTGGGTGAATCAGCAGCACCCCATCCCGGCTTTGCGCACCGCTCTGGCCCCCCAGCCCATTCAACTGGCCAC
CTCCGGTGGCCAAAACCCCCCGGCCAGCGAGGGTGGTGTGGACTTTCTGCGGGTCGTCGGATCCCCGGAAGAGGAGCTGC
AACAGGTGGCGCGGCAGGCCATTGCAGCCGCCCAAGCTTTTCCCGGCCAAACGGTGGCCATTCTGGTCACCACCAACGAG
CTGGGGGGCAAAGCCCTCAGCTATCTCCAGGAAATGGGAGCGGAGCGGGTGATCGATCTGTTGCGGGGCAGTCCCAGTCA
GCGGCGGGTGTTGTTGCAGCTCAAGGGAATCATCGACTGGTTTGCCCAGCCCACTGCCCCCACTCGCTTGGCTGCTGCTG
TGGATTGCTTGGCGGATTGGGCGGGGATCCCACGTGCTGAACTGGGGCCGATGTTGACCTGGATCCGCGCCTCTCTGCCG
GAGCGGCTGCTATTTCCCAACTTTGGTGAGGCTCCCGCTCTGCCCGATGGCCCGCCTTGGCAGAACTTGGGCAGGCTGCT
GCGCACCCTTGCCGGTTGGCTCCGGGCCGGGCGCTCCCCTTGGCCGGAGGTGCTGAATGTGATCGTTCAAAGCCTCTACC
GCAAGCCGGAAGAGCTGTTTGTGGGTCGCTATGTAATCGATCAGCTGGAGCAGGTGCTGGGATCCCTGCCCGAAACCGAT
TGGCAGGAGATCGCCCAAGAATTTCAAGTGATTCTGGATCACCGCCTCAACAACTTGCCTTCGGAGATCTTCGCCTTCGA
CCCCGAGCCGGGCAGCATCACTGTCACGACTCTGCATCGGGCCAAGGGGCTGGAATGGGATCAGGTGTTCATCCCCCAGG
TGTCGGCCTACGAGTTTCCCACCCAGTACGGGGAGCGAACCCTGGGGCTGAAGTTTTTGGATGAAGTGGATATGCAGGCA
GAGGCCCTGGCTCAGTTGCGACAGCTCAGCCCCAAAGGCGGCTGGGAGCTGCAGGCCGACTCGGCCACCAAGCAAGCCTT
TTTGGATTTGGCAGCAGAGCGATTGCGGCTGCTGTATGTGGGCATCACCCGCAGCAAACGACGGCTCACCCTCTCGGTAT
CCAGCCAATCTCGCTTTGGCACAGAGCAGAGTTCATCTTTGGTGTTTCGGGTGTTAAGTCGCAGTTTTAGGGCCGGTTGA

Upstream 100 bases:

>100_bases
CAGCTCTCTTGCCCCATGCCGATTGGATTGCCCCTCCTGTGGCAGAAGACGGGGTGGCGGCAGCCATCGAGACCTTCATC
CTCAGCTAGGCAGGGATCCC

Downstream 100 bases:

>100_bases
CCGGGTGAGTCTCTCGTTCTTCATGGATTGGCAACCTGCTAGCATGGCTAAGCCCGATAGCGCTTGCGCGCCGCCTTGCG
GCAATGGGGCAGTCTTTCAG

Product: UvrD/Rep family helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MLGLASSPFPEVTLRPAQQQALAYQSGPLGIAAVPGSGKTFILELLIADLILNRGIPPERIGVFTYMRSARGNLISRINQ
RLQRAGYPDRFTQAFTLHSLSLRVLREAPSRWEELSILEGYEQERLLSRLCRAWLVRHTQLWEPLIPGDNNPQKIAQNRA
QFRRSFQQMVQVVISRAKNLRLGPEVIPSEGYLGWAAPIYAGYQAELRRLGKLDYDDLGWQAVDLIEGSPELREQVQNWY
DYLFEDEAQDSSPLQEELLRLLSQRTGNLVRVGDPNQSIMGTFTSAEPRLFRAFCQASPQVVLLDESSRSAPQIIALANR
LVEWVNQQHPIPALRTALAPQPIQLATSGGQNPPASEGGVDFLRVVGSPEEELQQVARQAIAAAQAFPGQTVAILVTTNE
LGGKALSYLQEMGAERVIDLLRGSPSQRRVLLQLKGIIDWFAQPTAPTRLAAAVDCLADWAGIPRAELGPMLTWIRASLP
ERLLFPNFGEAPALPDGPPWQNLGRLLRTLAGWLRAGRSPWPEVLNVIVQSLYRKPEELFVGRYVIDQLEQVLGSLPETD
WQEIAQEFQVILDHRLNNLPSEIFAFDPEPGSITVTTLHRAKGLEWDQVFIPQVSAYEFPTQYGERTLGLKFLDEVDMQA
EALAQLRQLSPKGGWELQADSATKQAFLDLAAERLRLLYVGITRSKRRLTLSVSSQSRFGTEQSSSLVFRVLSRSFRAG

Sequences:

>Translated_719_residues
MLGLASSPFPEVTLRPAQQQALAYQSGPLGIAAVPGSGKTFILELLIADLILNRGIPPERIGVFTYMRSARGNLISRINQ
RLQRAGYPDRFTQAFTLHSLSLRVLREAPSRWEELSILEGYEQERLLSRLCRAWLVRHTQLWEPLIPGDNNPQKIAQNRA
QFRRSFQQMVQVVISRAKNLRLGPEVIPSEGYLGWAAPIYAGYQAELRRLGKLDYDDLGWQAVDLIEGSPELREQVQNWY
DYLFEDEAQDSSPLQEELLRLLSQRTGNLVRVGDPNQSIMGTFTSAEPRLFRAFCQASPQVVLLDESSRSAPQIIALANR
LVEWVNQQHPIPALRTALAPQPIQLATSGGQNPPASEGGVDFLRVVGSPEEELQQVARQAIAAAQAFPGQTVAILVTTNE
LGGKALSYLQEMGAERVIDLLRGSPSQRRVLLQLKGIIDWFAQPTAPTRLAAAVDCLADWAGIPRAELGPMLTWIRASLP
ERLLFPNFGEAPALPDGPPWQNLGRLLRTLAGWLRAGRSPWPEVLNVIVQSLYRKPEELFVGRYVIDQLEQVLGSLPETD
WQEIAQEFQVILDHRLNNLPSEIFAFDPEPGSITVTTLHRAKGLEWDQVFIPQVSAYEFPTQYGERTLGLKFLDEVDMQA
EALAQLRQLSPKGGWELQADSATKQAFLDLAAERLRLLYVGITRSKRRLTLSVSSQSRFGTEQSSSLVFRVLSRSFRAG
>Mature_719_residues
MLGLASSPFPEVTLRPAQQQALAYQSGPLGIAAVPGSGKTFILELLIADLILNRGIPPERIGVFTYMRSARGNLISRINQ
RLQRAGYPDRFTQAFTLHSLSLRVLREAPSRWEELSILEGYEQERLLSRLCRAWLVRHTQLWEPLIPGDNNPQKIAQNRA
QFRRSFQQMVQVVISRAKNLRLGPEVIPSEGYLGWAAPIYAGYQAELRRLGKLDYDDLGWQAVDLIEGSPELREQVQNWY
DYLFEDEAQDSSPLQEELLRLLSQRTGNLVRVGDPNQSIMGTFTSAEPRLFRAFCQASPQVVLLDESSRSAPQIIALANR
LVEWVNQQHPIPALRTALAPQPIQLATSGGQNPPASEGGVDFLRVVGSPEEELQQVARQAIAAAQAFPGQTVAILVTTNE
LGGKALSYLQEMGAERVIDLLRGSPSQRRVLLQLKGIIDWFAQPTAPTRLAAAVDCLADWAGIPRAELGPMLTWIRASLP
ERLLFPNFGEAPALPDGPPWQNLGRLLRTLAGWLRAGRSPWPEVLNVIVQSLYRKPEELFVGRYVIDQLEQVLGSLPETD
WQEIAQEFQVILDHRLNNLPSEIFAFDPEPGSITVTTLHRAKGLEWDQVFIPQVSAYEFPTQYGERTLGLKFLDEVDMQA
EALAQLRQLSPKGGWELQADSATKQAFLDLAAERLRLLYVGITRSKRRLTLSVSSQSRFGTEQSSSLVFRVLSRSFRAG

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=318, Percent_Identity=28.3018867924528, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80393; Mature: 80393

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLASSPFPEVTLRPAQQQALAYQSGPLGIAAVPGSGKTFILELLIADLILNRGIPPER
CCCCCCCCCCCCEECCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHH
IGVFTYMRSARGNLISRINQRLQRAGYPDRFTQAFTLHSLSLRVLREAPSRWEELSILEG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
YEQERLLSRLCRAWLVRHTQLWEPLIPGDNNPQKIAQNRAQFRRSFQQMVQVVISRAKNL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLGPEVIPSEGYLGWAAPIYAGYQAELRRLGKLDYDDLGWQAVDLIEGSPELREQVQNWY
CCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
DYLFEDEAQDSSPLQEELLRLLSQRTGNLVRVGDPNQSIMGTFTSAEPRLFRAFCQASPQ
HHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCHHHHHHHHHCCCC
VVLLDESSRSAPQIIALANRLVEWVNQQHPIPALRTALAPQPIQLATSGGQNPPASEGGV
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCH
DFLRVVGSPEEELQQVARQAIAAAQAFPGQTVAILVTTNELGGKALSYLQEMGAERVIDL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCHHHHHHH
LRGSPSQRRVLLQLKGIIDWFAQPTAPTRLAAAVDCLADWAGIPRAELGPMLTWIRASLP
HHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
ERLLFPNFGEAPALPDGPPWQNLGRLLRTLAGWLRAGRSPWPEVLNVIVQSLYRKPEELF
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHH
VGRYVIDQLEQVLGSLPETDWQEIAQEFQVILDHRLNNLPSEIFAFDPEPGSITVTTLHR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCEEEEEEHH
AKGLEWDQVFIPQVSAYEFPTQYGERTLGLKFLDEVDMQAEALAQLRQLSPKGGWELQAD
HCCCCCCCEECCCCHHHCCCHHHCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SATKQAFLDLAAERLRLLYVGITRSKRRLTLSVSSQSRFGTEQSSSLVFRVLSRSFRAG
HHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGLASSPFPEVTLRPAQQQALAYQSGPLGIAAVPGSGKTFILELLIADLILNRGIPPER
CCCCCCCCCCCCEECCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHH
IGVFTYMRSARGNLISRINQRLQRAGYPDRFTQAFTLHSLSLRVLREAPSRWEELSILEG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
YEQERLLSRLCRAWLVRHTQLWEPLIPGDNNPQKIAQNRAQFRRSFQQMVQVVISRAKNL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLGPEVIPSEGYLGWAAPIYAGYQAELRRLGKLDYDDLGWQAVDLIEGSPELREQVQNWY
CCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
DYLFEDEAQDSSPLQEELLRLLSQRTGNLVRVGDPNQSIMGTFTSAEPRLFRAFCQASPQ
HHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCHHHHHHHHHCCCC
VVLLDESSRSAPQIIALANRLVEWVNQQHPIPALRTALAPQPIQLATSGGQNPPASEGGV
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCH
DFLRVVGSPEEELQQVARQAIAAAQAFPGQTVAILVTTNELGGKALSYLQEMGAERVIDL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCHHHHHHH
LRGSPSQRRVLLQLKGIIDWFAQPTAPTRLAAAVDCLADWAGIPRAELGPMLTWIRASLP
HHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
ERLLFPNFGEAPALPDGPPWQNLGRLLRTLAGWLRAGRSPWPEVLNVIVQSLYRKPEELF
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHH
VGRYVIDQLEQVLGSLPETDWQEIAQEFQVILDHRLNNLPSEIFAFDPEPGSITVTTLHR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCEEEEEEHH
AKGLEWDQVFIPQVSAYEFPTQYGERTLGLKFLDEVDMQAEALAQLRQLSPKGGWELQAD
HCCCCCCCEECCCCHHHCCCHHHCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SATKQAFLDLAAERLRLLYVGITRSKRRLTLSVSSQSRFGTEQSSSLVFRVLSRSFRAG
HHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA