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The map label for this gene is purB [H]

Identifier: 86609374

GI number: 86609374

Start: 2002741

End: 2004036

Strand: Reverse

Name: purB [H]

Synonym: CYB_1918

Alternate gene names: 86609374

Gene position: 2004036-2002741 (Counterclockwise)

Preceding gene: 86609377

Following gene: 86609373

Centisome position: 65.78

GC content: 61.65

Gene sequence:

>1296_bases
TTGATCGAGCGCTACACTCTGCCCGAAATGGGCCAGATTTGGTCCACTGCCCACAAGCTCCAGACCTGGCTGCAGGTGGA
GCTGGCTGTCTGTGAAGCGCAAGCGGAGCTGGGGCGGATCCCGCAAGAAGCCCTGCAGGAGATCAGAGCCAAAGCCGCCT
TTGACCCGGCGCGGGTTTTGGAAATTGAGGCGGAAGTGAAGCACGATGTTATTGCCTTCTTAACCAACCTCAACGAGCAC
GTGGGCGAGGCAGGGCGCTACATCCACCTGGGCCTGACCAGCTCGGATGTGTTGGATACGGCCCTGGCCCTGCAGCTTGT
GGCCTCGGTGGAGATCTTGCAAGCCCGCCTGGCCGACCTCATCGCTGCTATCCGCGACCAAGCTCGGCAGCACCGCTACA
CCCTCATGATCGGCCGCACCCACGGTGTTCACGCCGAGCCGATTACCTTTGGCTTTAAGCTGGCAGGGTGGCTGGCCGAG
TGCCTGCGCCACCAAGAGCGCCTAGCTCGCCTGCGGGAAGTGGTGGCGGTGGGCAAGATCTCTGGCGCCGTGGGCACCTA
CGCCAACGTGCCTCCCCAGGTGGAGCAGTTGGCCTGCGCTCGCCTGGGCCTGAAGCCCGATACAGCCTCGACACAGGTAA
TCTCGCGGGATCGCCACGCTGAATACGTGCAAACCTTGGCTCTGATTGGCTCTTCCCTAGAGCGCTTTGCTACTGAAATC
CGCCACCTGCAGCGAACAGAGGTACTGGAGGCTGAGGAGTACTTTGCCCCCGGCCAAAAGGGATCCTCTGCTATGCCCCA
CAAGCGCAACCCCATCAAATCTGAACAGCTAACCGGGATTTCCCGCCTCCTGCGGGGGTATGCCCTTCCGGCTCTGGAAA
ACATTCCTCTTTGGCATGAGCGGGACATCTCCCACAGCTCCGTAGAGCGGGTCATCCTGCCGGATGCCTCCATCCTCTGC
CACTATCTGTTGGTCAACATGGCGGAGCTGGTGGGCCAGCTGCAGGTTCACGCTCGCAACATGGAGCGCAATCTCAACCT
CTACGGCGGCGTCATCTTCAGCCAGCAGGTGCTGCTGGCTTTGGTGGAGAAGGGCCTTAGCCGCGAGGAAGCCTACCGCC
TGGTGCAGGAAAATGCTCACCGCGCCTGGAACCAGCCCGGCGGGGATTTCCGCGCCAATCTGCTGGCGGATCCCCGAGTG
ATGGCCCACCTCAGCGAGGCGGAACTGCAGGCTTGTTTTGATCCGCGGCGCCACCTAACCCATTTGGACGAGATCTTTGC
CCGCTTGGGCATTTGA

Upstream 100 bases:

>100_bases
AGCCAAGCCCGCAGGGATCCCCCAGCGGCTTTTCCTCGAGGGGGGACGCCAAAGGCTAAAATAGCCTAGCTCATCCTACT
GTACCGACGGAAAAGGCACC

Downstream 100 bases:

>100_bases
GGATCCCCTATGGTTTGGCAGCGGCAGCACGTCTTGGGCTTGGCGGATTTTACTCCCGAAGAATACCAGATGGTTTTGCA
GACCAGCGCCAGTTTCCAGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGQIWSTAHKLQTWLQVELAVCEAQAELGRIPQEALQEIRAKAAFDPARVLEIEAEVKHDVIAFLTNLNEH
VGEAGRYIHLGLTSSDVLDTALALQLVASVEILQARLADLIAAIRDQARQHRYTLMIGRTHGVHAEPITFGFKLAGWLAE
CLRHQERLARLREVVAVGKISGAVGTYANVPPQVEQLACARLGLKPDTASTQVISRDRHAEYVQTLALIGSSLERFATEI
RHLQRTEVLEAEEYFAPGQKGSSAMPHKRNPIKSEQLTGISRLLRGYALPALENIPLWHERDISHSSVERVILPDASILC
HYLLVNMAELVGQLQVHARNMERNLNLYGGVIFSQQVLLALVEKGLSREEAYRLVQENAHRAWNQPGGDFRANLLADPRV
MAHLSEAELQACFDPRRHLTHLDEIFARLGI

Sequences:

>Translated_431_residues
MIERYTLPEMGQIWSTAHKLQTWLQVELAVCEAQAELGRIPQEALQEIRAKAAFDPARVLEIEAEVKHDVIAFLTNLNEH
VGEAGRYIHLGLTSSDVLDTALALQLVASVEILQARLADLIAAIRDQARQHRYTLMIGRTHGVHAEPITFGFKLAGWLAE
CLRHQERLARLREVVAVGKISGAVGTYANVPPQVEQLACARLGLKPDTASTQVISRDRHAEYVQTLALIGSSLERFATEI
RHLQRTEVLEAEEYFAPGQKGSSAMPHKRNPIKSEQLTGISRLLRGYALPALENIPLWHERDISHSSVERVILPDASILC
HYLLVNMAELVGQLQVHARNMERNLNLYGGVIFSQQVLLALVEKGLSREEAYRLVQENAHRAWNQPGGDFRANLLADPRV
MAHLSEAELQACFDPRRHLTHLDEIFARLGI
>Mature_431_residues
MIERYTLPEMGQIWSTAHKLQTWLQVELAVCEAQAELGRIPQEALQEIRAKAAFDPARVLEIEAEVKHDVIAFLTNLNEH
VGEAGRYIHLGLTSSDVLDTALALQLVASVEILQARLADLIAAIRDQARQHRYTLMIGRTHGVHAEPITFGFKLAGWLAE
CLRHQERLARLREVVAVGKISGAVGTYANVPPQVEQLACARLGLKPDTASTQVISRDRHAEYVQTLALIGSSLERFATEI
RHLQRTEVLEAEEYFAPGQKGSSAMPHKRNPIKSEQLTGISRLLRGYALPALENIPLWHERDISHSSVERVILPDASILC
HYLLVNMAELVGQLQVHARNMERNLNLYGGVIFSQQVLLALVEKGLSREEAYRLVQENAHRAWNQPGGDFRANLLADPRV
MAHLSEAELQACFDPRRHLTHLDEIFARLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=431, Percent_Identity=28.0742459396752, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI183227688, Length=391, Percent_Identity=28.1329923273657, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1787376, Length=315, Percent_Identity=30.4761904761905, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790398, Length=436, Percent_Identity=27.2935779816514, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=390, Percent_Identity=28.974358974359, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564234, Length=335, Percent_Identity=30.7462686567164, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=27.5252525252525, Blast_Score=136, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24647570, Length=403, Percent_Identity=26.7990074441687, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI221473854, Length=255, Percent_Identity=27.4509803921569, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI78706858, Length=255, Percent_Identity=27.4509803921569, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48264; Mature: 48264

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGQIWSTAHKLQTWLQVELAVCEAQAELGRIPQEALQEIRAKAAFDPARVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHE
EIEAEVKHDVIAFLTNLNEHVGEAGRYIHLGLTSSDVLDTALALQLVASVEILQARLADL
EEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAIRDQARQHRYTLMIGRTHGVHAEPITFGFKLAGWLAECLRHQERLARLREVVAVGKI
HHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANVPPQVEQLACARLGLKPDTASTQVISRDRHAEYVQTLALIGSSLERFATEI
HCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEYFAPGQKGSSAMPHKRNPIKSEQLTGISRLLRGYALPALENIPLWHE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
RDISHSSVERVILPDASILCHYLLVNMAELVGQLQVHARNMERNLNLYGGVIFSQQVLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LVEKGLSREEAYRLVQENAHRAWNQPGGDFRANLLADPRVMAHLSEAELQACFDPRRHLT
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCHHHHHH
HLDEIFARLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGQIWSTAHKLQTWLQVELAVCEAQAELGRIPQEALQEIRAKAAFDPARVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHE
EIEAEVKHDVIAFLTNLNEHVGEAGRYIHLGLTSSDVLDTALALQLVASVEILQARLADL
EEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAIRDQARQHRYTLMIGRTHGVHAEPITFGFKLAGWLAECLRHQERLARLREVVAVGKI
HHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANVPPQVEQLACARLGLKPDTASTQVISRDRHAEYVQTLALIGSSLERFATEI
HCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEYFAPGQKGSSAMPHKRNPIKSEQLTGISRLLRGYALPALENIPLWHE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
RDISHSSVERVILPDASILCHYLLVNMAELVGQLQVHARNMERNLNLYGGVIFSQQVLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LVEKGLSREEAYRLVQENAHRAWNQPGGDFRANLLADPRVMAHLSEAELQACFDPRRHLT
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCHHHHHH
HLDEIFARLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]