Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 86609377

GI number: 86609377

Start: 2005832

End: 2006620

Strand: Reverse

Name: lipB

Synonym: CYB_1921

Alternate gene names: 86609377

Gene position: 2006620-2005832 (Counterclockwise)

Preceding gene: 86609378

Following gene: 86609374

Centisome position: 65.86

GC content: 61.34

Gene sequence:

>789_bases
GTGAACTTTTCTGGATCCCCCGTGCAACTGCCGCTTCTGCAGGTTCACCTGCCGGGTGAAGTGCCCTACCGCACTGCCTG
GGCTTGGCAGCAGGCAAGACTGGCCCATATGATCCGGGATCCCCAGTTGCCGGACGGATTACTGCTGCTCACCCATCCTG
CGGTTTACACCTTGGGGGCAGGAGCCGATCCCAAGTTTCTCAAGAGCCTTTCTCAGCCGATAGGATCCCCGGCTATCCAC
CCGGAATCTCCTTCTGAACAAGAGCCGGAAATCCTACGGGTGGAGCGCGGCGGCGAAGTGACCTACCATGGGCCGGGGCA
GTGGGTGGGCTATGCGATGCTGAACCTAAAGCGCCACCGCCCAGATCTCCACGAGTACCTACGCCAGCTAGAAGAGGTGG
TCATCCAGACCTTGGCCCACTTCGGCCTACAGGGGGAGCGGATCCCTGGCTTGACAGGAGTTTGGGTGCAGGGCCGCAAA
GTGGCGGCCATCGGCATCAAGGTCTCCCGCTGGGTGACCTACCACGGCTTTGCCCTCAACGTCTGCCCGGATCTAGCTGC
CTTCGAGGCCATCGTGCCCTGTGGGATCCCTGATCGCCCGGTGGGATCCCTGGTGCACTTTTGTCCGGAGGTAACGATGG
CTGCCGTAGCTCCGGTGCTGGTGGAGTCGTTCTGTCAGGTCTTTGGGCTGCAGGCTCAGGCAGTTTCCCTGGCCGAGTGG
TTGGGAGAGCCTCGGCTTTCTGACAAGTTGCTGGGAGAATCAACCGCTCAGACGCCTAAGCGCAAGTGA

Upstream 100 bases:

>100_bases
TTCAAAGATGGGAAGCCGATTGACCGCATTGAGGGGCTGTTGCAGCCGCGGGCCCTGTTGGAGCGGTTGCGGGCGCAGGT
TAACTAAACCTGTAAGCTAG

Downstream 100 bases:

>100_bases
GGGGAAAGACCAGACAGGTTACCCAACTTCGAGGTGGCCCTAGGTTTAATCCAAGGGCGGCTACTCCACCCCCAACTGGC
GCAGCAGTTGCTGCAGGATG

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNFSGSPVQLPLLQVHLPGEVPYRTAWAWQQARLAHMIRDPQLPDGLLLLTHPAVYTLGAGADPKFLKSLSQPIGSPAIH
PESPSEQEPEILRVERGGEVTYHGPGQWVGYAMLNLKRHRPDLHEYLRQLEEVVIQTLAHFGLQGERIPGLTGVWVQGRK
VAAIGIKVSRWVTYHGFALNVCPDLAAFEAIVPCGIPDRPVGSLVHFCPEVTMAAVAPVLVESFCQVFGLQAQAVSLAEW
LGEPRLSDKLLGESTAQTPKRK

Sequences:

>Translated_262_residues
MNFSGSPVQLPLLQVHLPGEVPYRTAWAWQQARLAHMIRDPQLPDGLLLLTHPAVYTLGAGADPKFLKSLSQPIGSPAIH
PESPSEQEPEILRVERGGEVTYHGPGQWVGYAMLNLKRHRPDLHEYLRQLEEVVIQTLAHFGLQGERIPGLTGVWVQGRK
VAAIGIKVSRWVTYHGFALNVCPDLAAFEAIVPCGIPDRPVGSLVHFCPEVTMAAVAPVLVESFCQVFGLQAQAVSLAEW
LGEPRLSDKLLGESTAQTPKRK
>Mature_262_residues
MNFSGSPVQLPLLQVHLPGEVPYRTAWAWQQARLAHMIRDPQLPDGLLLLTHPAVYTLGAGADPKFLKSLSQPIGSPAIH
PESPSEQEPEILRVERGGEVTYHGPGQWVGYAMLNLKRHRPDLHEYLRQLEEVVIQTLAHFGLQGERIPGLTGVWVQGRK
VAAIGIKVSRWVTYHGFALNVCPDLAAFEAIVPCGIPDRPVGSLVHFCPEVTMAAVAPVLVESFCQVFGLQAQAVSLAEW
LGEPRLSDKLLGESTAQTPKRK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=223, Percent_Identity=33.6322869955157, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081767, Length=191, Percent_Identity=32.9842931937173, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323268, Length=167, Percent_Identity=32.9341317365269, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355483, Length=241, Percent_Identity=34.0248962655602, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_SYNJB (Q2JKB7)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478139.1
- ProteinModelPortal:   Q2JKB7
- SMR:   Q2JKB7
- STRING:   Q2JKB7
- GeneID:   3900754
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1921
- TIGR:   CYB_1921
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   ILRVERG
- PhylomeDB:   Q2JKB7
- ProtClustDB:   CLSK2516604
- BioCyc:   SSP321332:CYB_1921-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 28759; Mature: 28759

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSGSPVQLPLLQVHLPGEVPYRTAWAWQQARLAHMIRDPQLPDGLLLLTHPAVYTLGA
CCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECC
GADPKFLKSLSQPIGSPAIHPESPSEQEPEILRVERGGEVTYHGPGQWVGYAMLNLKRHR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHCC
PDLHEYLRQLEEVVIQTLAHFGLQGERIPGLTGVWVQGRKVAAIGIKVSRWVTYHGFALN
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEEEEEHEEEECCEEEE
VCPDLAAFEAIVPCGIPDRPVGSLVHFCPEVTMAAVAPVLVESFCQVFGLQAQAVSLAEW
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LGEPRLSDKLLGESTAQTPKRK
HCCCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNFSGSPVQLPLLQVHLPGEVPYRTAWAWQQARLAHMIRDPQLPDGLLLLTHPAVYTLGA
CCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECC
GADPKFLKSLSQPIGSPAIHPESPSEQEPEILRVERGGEVTYHGPGQWVGYAMLNLKRHR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHCC
PDLHEYLRQLEEVVIQTLAHFGLQGERIPGLTGVWVQGRKVAAIGIKVSRWVTYHGFALN
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEEEEEHEEEECCEEEE
VCPDLAAFEAIVPCGIPDRPVGSLVHFCPEVTMAAVAPVLVESFCQVFGLQAQAVSLAEW
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LGEPRLSDKLLGESTAQTPKRK
HCCCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA