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The map label for this gene is pyrB [H]

Identifier: 86609373

GI number: 86609373

Start: 2001751

End: 2002731

Strand: Reverse

Name: pyrB [H]

Synonym: CYB_1917

Alternate gene names: 86609373

Gene position: 2002731-2001751 (Counterclockwise)

Preceding gene: 86609374

Following gene: 86609371

Centisome position: 65.73

GC content: 60.75

Gene sequence:

>981_bases
ATGGTTTGGCAGCGGCAGCACGTCTTGGGCTTGGCGGATTTTACTCCCGAAGAATACCAGATGGTTTTGCAGACCAGCGC
CAGTTTCCAGGAGGTGTTGACGCGGCGGTTGCCCAAGGTGCCCACCTTGCAGGGGAAGGTGGTGGTGACGCTGTTCTTCG
AGCCTTCCACCCGCACCCGTACCAGTTTTGAGCTGGCGGCCAAGCGCCTTTCGGCAGATGTGCTCAACTTTTCCCCAGGC
ACCTCTTCCTTGAGCAAAGGGGAGACGCTTTTGGATACGGCCCGCACCTTTCTGGCCATGGGATCCGATCTCTTAATCGT
TCGCCATGCCCAGGCAGGAGTGCCCCAACAATTAGCTGCCGAGATCGACCGCCGCGGATCCCCTGTGGGCGTTCTCAATG
CCGGGGATGGACTGCACGAACATCCCACCCAAGGTTTGCTGGATTTGTTTACCCTCTGCAGCCATCTGGATCCCCGGCGT
CCCCGTCTGGAGTTGCTGCAAGGGATCAAAATCGCCATTGTCGGCGATATTCTCCACTCGCGGGTGGCCCGCTCCGATAT
CTATGCCTTGGTGGCCGCCGGAGCTGAGGTTCACTTAGCCGGCCCTCCCACCCTATTGCCCAAAGATTTTGCCCAGTTTG
TACCCGGCCACACGCTCCCTATCCATTGGCAGCTGGAGCCTGCCCTAGAAGGGGCGCGTTTTGTTATCACCTTGCGCCTG
CAACAGGAGCGCATGGGCGAGTTCCTTCTCCCCAGCTTGCAGGAGTACCATCACTTCTTCGGCCTCACGCGGCAGCGGCT
GCGCCTGTGCCACCCAGAGGTGAGGGTTTTACACCCCGGCCCGGTCAATCGCGGTGTGGAACTCAGCTCCGAGGTGATGG
AGGATCCCGGCTTGAACCTGATTGAGCAGCAGGTCACCCACGGCGTGGCGGTGCGCATGGCCCTGCTGTACCTCATGGGG
GGCGGGCGGATCCCAGCTTAG

Upstream 100 bases:

>100_bases
CCACCTCAGCGAGGCGGAACTGCAGGCTTGTTTTGATCCGCGGCGCCACCTAACCCATTTGGACGAGATCTTTGCCCGCT
TGGGCATTTGAGGATCCCCT

Downstream 100 bases:

>100_bases
AGCCCAGATCTTTCTCTGCTCGGGGAACATGTAGAGCTGCCCCACGTCTGGAGTGTTGAAGTCCATCTCCTAGCTAATTC
TCTCTGTCAAAACTGTCAAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MVWQRQHVLGLADFTPEEYQMVLQTSASFQEVLTRRLPKVPTLQGKVVVTLFFEPSTRTRTSFELAAKRLSADVLNFSPG
TSSLSKGETLLDTARTFLAMGSDLLIVRHAQAGVPQQLAAEIDRRGSPVGVLNAGDGLHEHPTQGLLDLFTLCSHLDPRR
PRLELLQGIKIAIVGDILHSRVARSDIYALVAAGAEVHLAGPPTLLPKDFAQFVPGHTLPIHWQLEPALEGARFVITLRL
QQERMGEFLLPSLQEYHHFFGLTRQRLRLCHPEVRVLHPGPVNRGVELSSEVMEDPGLNLIEQQVTHGVAVRMALLYLMG
GGRIPA

Sequences:

>Translated_326_residues
MVWQRQHVLGLADFTPEEYQMVLQTSASFQEVLTRRLPKVPTLQGKVVVTLFFEPSTRTRTSFELAAKRLSADVLNFSPG
TSSLSKGETLLDTARTFLAMGSDLLIVRHAQAGVPQQLAAEIDRRGSPVGVLNAGDGLHEHPTQGLLDLFTLCSHLDPRR
PRLELLQGIKIAIVGDILHSRVARSDIYALVAAGAEVHLAGPPTLLPKDFAQFVPGHTLPIHWQLEPALEGARFVITLRL
QQERMGEFLLPSLQEYHHFFGLTRQRLRLCHPEVRVLHPGPVNRGVELSSEVMEDPGLNLIEQQVTHGVAVRMALLYLMG
GGRIPA
>Mature_326_residues
MVWQRQHVLGLADFTPEEYQMVLQTSASFQEVLTRRLPKVPTLQGKVVVTLFFEPSTRTRTSFELAAKRLSADVLNFSPG
TSSLSKGETLLDTARTFLAMGSDLLIVRHAQAGVPQQLAAEIDRRGSPVGVLNAGDGLHEHPTQGLLDLFTLCSHLDPRR
PRLELLQGIKIAIVGDILHSRVARSDIYALVAAGAEVHLAGPPTLLPKDFAQFVPGHTLPIHWQLEPALEGARFVITLRL
QQERMGEFLLPSLQEYHHFFGLTRQRLRLCHPEVRVLHPGPVNRGVELSSEVMEDPGLNLIEQQVTHGVAVRMALLYLMG
GGRIPA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=317, Percent_Identity=35.0157728706625, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI2367364, Length=324, Percent_Identity=33.0246913580247, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1790703, Length=311, Percent_Identity=28.2958199356913, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786469, Length=350, Percent_Identity=25.1428571428571, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=29.4117647058824, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322331, Length=323, Percent_Identity=30.9597523219814, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24642586, Length=327, Percent_Identity=29.9694189602446, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 36054; Mature: 36054

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWQRQHVLGLADFTPEEYQMVLQTSASFQEVLTRRLPKVPTLQGKVVVTLFFEPSTRTR
CCCCCCHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCH
TSFELAAKRLSADVLNFSPGTSSLSKGETLLDTARTFLAMGSDLLIVRHAQAGVPQQLAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
EIDRRGSPVGVLNAGDGLHEHPTQGLLDLFTLCSHLDPRRPRLELLQGIKIAIVGDILHS
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHEEHHHHHHH
RVARSDIYALVAAGAEVHLAGPPTLLPKDFAQFVPGHTLPIHWQLEPALEGARFVITLRL
HHHHHHHHHHEECCCEEEECCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEE
QQERMGEFLLPSLQEYHHFFGLTRQRLRLCHPEVRVLHPGPVNRGVELSSEVMEDPGLNL
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCHH
IEQQVTHGVAVRMALLYLMGGGRIPA
HHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVWQRQHVLGLADFTPEEYQMVLQTSASFQEVLTRRLPKVPTLQGKVVVTLFFEPSTRTR
CCCCCCHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCH
TSFELAAKRLSADVLNFSPGTSSLSKGETLLDTARTFLAMGSDLLIVRHAQAGVPQQLAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
EIDRRGSPVGVLNAGDGLHEHPTQGLLDLFTLCSHLDPRRPRLELLQGIKIAIVGDILHS
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHEEHHHHHHH
RVARSDIYALVAAGAEVHLAGPPTLLPKDFAQFVPGHTLPIHWQLEPALEGARFVITLRL
HHHHHHHHHHEECCCEEEECCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEE
QQERMGEFLLPSLQEYHHFFGLTRQRLRLCHPEVRVLHPGPVNRGVELSSEVMEDPGLNL
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCHH
IEQQVTHGVAVRMALLYLMGGGRIPA
HHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA