Click here to switch to the map view.

The map label for this gene is nikE [H]

Identifier: 86609337

GI number: 86609337

Start: 1965810

End: 1966484

Strand: Reverse

Name: nikE [H]

Synonym: CYB_1881

Alternate gene names: 86609337

Gene position: 1966484-1965810 (Counterclockwise)

Preceding gene: 86609338

Following gene: 86609336

Centisome position: 64.55

GC content: 58.52

Gene sequence:

>675_bases
ATGCTCAAGGGTGAGCATCTCTGGTTTCGCTATGGACGGCATCTGCCTTGGGTGGTGCAAGATCAGACCCTTCAAGTAGA
ACTTGGGGAAGTCGTAGGGCTGATGGCTCCCTCTGGTTTTGGCAAAACTACTCTGGCCAAGTTGCTGGCGGGCTACCTTA
CCCCCAGCCAAGGTCGGGTGAGTGTGACAGGGCAGCCTTTGCCTCGGCGAGGGTACTGCCCAGTGCAGCTTGTCTTTCAA
CATCCAGAGCAGGCCGTTAACCCCCACTGGCGCATAGCTCAGGTCTTGCGGGAAGGGCCTCCTCCCCAGCCAGAGGTTCT
CCACACCTTAGGCATTTCCCCCAGTTGGCTCAACCGCTATCCCCATGAGCTAAGCGGGGGAGAATTGCAGCGGGTTGTCA
TCGCCCGGGTTCTCAACCCACAAACTCGCTATTTGATTGCAGATGAGATGACAGCCATGCTCGACGCCAATACCCAAGCC
CTGATCTGGCAAGCTGTCTTGGCCTATGGGCGGGCACATCAGATGGGGCTTTTGGTCATCAGTCATGATCGCTTTCTCCT
GGAACGGCTCTGCAGCCGCTACGTCATCATGACCAGCCATGACCCCTATCTGGTGGAGCGGGCCGGCCACCGGGTGGTGC
GCATGGAGGGAGGGCGCCTGTATGATCTGCGCTGA

Upstream 100 bases:

>100_bases
GGTTGTTTGTTTGGGGATCGCTGCCCCTGGGCAAGTGAAATCTGTCGAGCTGAGCATCCGCCTCTGCGGGCGGTTGGAGG
AGGGTGGGTGAGGTGTATCC

Downstream 100 bases:

>100_bases
GGGCTGCCGGGATCCCTATTTTGCTCTCCACAGGCTCTGTTGAGCTCCAGGGGGCAAGGGTCTGAGCCCATTGATTGTTG
GATGGAGAACAGCATGCAAT

Product: peptide/opine/nickel uptake ABC transporter ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLKGEHLWFRYGRHLPWVVQDQTLQVELGEVVGLMAPSGFGKTTLAKLLAGYLTPSQGRVSVTGQPLPRRGYCPVQLVFQ
HPEQAVNPHWRIAQVLREGPPPQPEVLHTLGISPSWLNRYPHELSGGELQRVVIARVLNPQTRYLIADEMTAMLDANTQA
LIWQAVLAYGRAHQMGLLVISHDRFLLERLCSRYVIMTSHDPYLVERAGHRVVRMEGGRLYDLR

Sequences:

>Translated_224_residues
MLKGEHLWFRYGRHLPWVVQDQTLQVELGEVVGLMAPSGFGKTTLAKLLAGYLTPSQGRVSVTGQPLPRRGYCPVQLVFQ
HPEQAVNPHWRIAQVLREGPPPQPEVLHTLGISPSWLNRYPHELSGGELQRVVIARVLNPQTRYLIADEMTAMLDANTQA
LIWQAVLAYGRAHQMGLLVISHDRFLLERLCSRYVIMTSHDPYLVERAGHRVVRMEGGRLYDLR
>Mature_224_residues
MLKGEHLWFRYGRHLPWVVQDQTLQVELGEVVGLMAPSGFGKTTLAKLLAGYLTPSQGRVSVTGQPLPRRGYCPVQLVFQ
HPEQAVNPHWRIAQVLREGPPPQPEVLHTLGISPSWLNRYPHELSGGELQRVVIARVLNPQTRYLIADEMTAMLDANTQA
LIWQAVLAYGRAHQMGLLVISHDRFLLERLCSRYVIMTSHDPYLVERAGHRVVRMEGGRLYDLR

Specific function: Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system [H]

COG id: COG1124

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1789891, Length=201, Percent_Identity=34.3283582089552, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788506, Length=200, Percent_Identity=35.5, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787758, Length=186, Percent_Identity=33.8709677419355, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787500, Length=219, Percent_Identity=28.310502283105, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787547, Length=218, Percent_Identity=26.605504587156, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1786253, Length=213, Percent_Identity=33.8028169014084, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787759, Length=207, Percent_Identity=30.4347826086957, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789962, Length=199, Percent_Identity=27.1356783919598, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693107, Length=191, Percent_Identity=30.3664921465969, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789963, Length=226, Percent_Identity=29.646017699115, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788165, Length=209, Percent_Identity=30.1435406698565, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786398, Length=207, Percent_Identity=27.0531400966184, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081791, Length=192, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787548, Length=221, Percent_Identity=26.6968325791855, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787370, Length=189, Percent_Identity=30.6878306878307, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081709, Length=185, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788450, Length=210, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789032, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789890, Length=205, Percent_Identity=30.2439024390244, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786563, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787089, Length=237, Percent_Identity=28.6919831223629, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786872, Length=179, Percent_Identity=29.0502793296089, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1790467, Length=201, Percent_Identity=29.8507462686567, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787182, Length=214, Percent_Identity=31.3084112149533, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI115533592, Length=244, Percent_Identity=28.6885245901639, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR015858
- InterPro:   IPR003593
- InterPro:   IPR014137 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: =3.6.3.24 [H]

Molecular weight: Translated: 25439; Mature: 25439

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGEHLWFRYGRHLPWVVQDQTLQVELGEVVGLMAPSGFGKTTLAKLLAGYLTPSQGRV
CCCCCEEHEECCCCCCEEEECCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEE
SVTGQPLPRRGYCPVQLVFQHPEQAVNPHWRIAQVLREGPPPQPEVLHTLGISPSWLNRY
EECCCCCCCCCCCCHHHEEECCHHHCCCHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHCC
PHELSGGELQRVVIARVLNPQTRYLIADEMTAMLDANTQALIWQAVLAYGRAHQMGLLVI
CCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEE
SHDRFLLERLCSRYVIMTSHDPYLVERAGHRVVRMEGGRLYDLR
ECCHHHHHHHHCCEEEEECCCCHHHHHCCCEEEEECCCEEECCC
>Mature Secondary Structure
MLKGEHLWFRYGRHLPWVVQDQTLQVELGEVVGLMAPSGFGKTTLAKLLAGYLTPSQGRV
CCCCCEEHEECCCCCCEEEECCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEE
SVTGQPLPRRGYCPVQLVFQHPEQAVNPHWRIAQVLREGPPPQPEVLHTLGISPSWLNRY
EECCCCCCCCCCCCHHHEEECCHHHCCCHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHCC
PHELSGGELQRVVIARVLNPQTRYLIADEMTAMLDANTQALIWQAVLAYGRAHQMGLLVI
CCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEE
SHDRFLLERLCSRYVIMTSHDPYLVERAGHRVVRMEGGRLYDLR
ECCHHHHHHHHCCEEEEECCCCHHHHHCCCEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]