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The map label for this gene is fcl [C]

Identifier: 86609020

GI number: 86609020

Start: 1628714

End: 1629673

Strand: Direct

Name: fcl [C]

Synonym: CYB_1556

Alternate gene names: 86609020

Gene position: 1628714-1629673 (Clockwise)

Preceding gene: 86609018

Following gene: 86609024

Centisome position: 53.46

GC content: 61.25

Gene sequence:

>960_bases
TTGCCCAGCTCCGTCGATCTCAGCCAGAAGCGCATTTTGCTCACAGGGGGCAGCGGCTTTTTGGGCAAACATGTGCTGCA
ACAGTTGCAGGTGCTGGGGGTAAAGCCGGAGCAGGTGCGCATCCCGCGCTCCCGCACCCAGGATCTGCGCCGTTGGGAAG
TCTGCCAAGAGGTGGTGCAAGGGCAAGATATCGTCATCCATTTGGCCGCCCACGTGGGTGGCATCGGCCTCAACCAAGCC
AAGCCGGCAGAACTCTTCTACGACAACCTCATCATGGGATCCCAGCTCATCCACGCCGCCTACCTGGCCGGGGTGGAGAA
GTTTGTCTGCGTGGGCACCATCTGCGCCTATCCCAAGTTCACCCCCGTTCCCTTCAAGGAAGAGGATCTCTGGAACGGCT
ACCCAGAGGAGACCAACGCCCCCTACGGCATCGCCAAGAAAGCCCTGCTGGTGCAGCTGCAGGCCTACCGGCAGCAGTAC
GGCTTCAACGGCATCTACCTGCTGCCGGTGAACCTCTACGGCCCCATGGACAACTTCGACCCCGAAAGCTCCCACGTCAT
CCCAGCCTTGATCCGCAAAGTGCACGAAGCGCAGCAACGGGGCCAAAACTACGTCGAAGTGTGGGGGGATGGTAGCCCCA
CCCGCGAGTTTTTCTACGCTGAAGATGCGGCCCGCGGCATTGTCATGGCCACCCAGCTTTACGACGGGGCGGATCCCTTG
AACCTGGGCACCGGTGAAGAGATCTCCATCCGGGATCTGATTGCCCTCATCTGCGAGCTGATGGAGTTCCGGGGAGAGAT
CCGCTGGCAAACGGACAAGCCCAATGGCCAACCCCGCCGCTGTCTGGATGTCTCCAAGGCCCGAGAGAGGATGGGTTTTG
TGGCCAAAGTCGGCCTGCGAGAAGGTCTGCAGCGCACCATCGACTGGTACCGCCGCCACGCCTGCTCTGGGCAAAGTTAG

Upstream 100 bases:

>100_bases
TATTCTCGATGACCATCGCCAACAAGTCTGGAATACGGCTATCCCGTCTGCAGCATAATGAAAGCCGGAAGTTCTCTGCG
TCGGATCTATGCCCAACAGC

Downstream 100 bases:

>100_bases
GCAACGGCTTGGCTCTCCAGCCGTCCATCTTCCATGTGCAGCACCCGATCTGCAATGTCCAGGATGCGGTTGTCGTGGGT
CACCATGAGGATGGTGCAAC

Product: NAD dependent epimerase/dehydratase family protein

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPSSVDLSQKRILLTGGSGFLGKHVLQQLQVLGVKPEQVRIPRSRTQDLRRWEVCQEVVQGQDIVIHLAAHVGGIGLNQA
KPAELFYDNLIMGSQLIHAAYLAGVEKFVCVGTICAYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLQAYRQQY
GFNGIYLLPVNLYGPMDNFDPESSHVIPALIRKVHEAQQRGQNYVEVWGDGSPTREFFYAEDAARGIVMATQLYDGADPL
NLGTGEEISIRDLIALICELMEFRGEIRWQTDKPNGQPRRCLDVSKARERMGFVAKVGLREGLQRTIDWYRRHACSGQS

Sequences:

>Translated_319_residues
MPSSVDLSQKRILLTGGSGFLGKHVLQQLQVLGVKPEQVRIPRSRTQDLRRWEVCQEVVQGQDIVIHLAAHVGGIGLNQA
KPAELFYDNLIMGSQLIHAAYLAGVEKFVCVGTICAYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLQAYRQQY
GFNGIYLLPVNLYGPMDNFDPESSHVIPALIRKVHEAQQRGQNYVEVWGDGSPTREFFYAEDAARGIVMATQLYDGADPL
NLGTGEEISIRDLIALICELMEFRGEIRWQTDKPNGQPRRCLDVSKARERMGFVAKVGLREGLQRTIDWYRRHACSGQS
>Mature_318_residues
PSSVDLSQKRILLTGGSGFLGKHVLQQLQVLGVKPEQVRIPRSRTQDLRRWEVCQEVVQGQDIVIHLAAHVGGIGLNQAK
PAELFYDNLIMGSQLIHAAYLAGVEKFVCVGTICAYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLQAYRQQYG
FNGIYLLPVNLYGPMDNFDPESSHVIPALIRKVHEAQQRGQNYVEVWGDGSPTREFFYAEDAARGIVMATQLYDGADPLN
LGTGEEISIRDLIALICELMEFRGEIRWQTDKPNGQPRRCLDVSKARERMGFVAKVGLREGLQRTIDWYRRHACSGQS

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=306, Percent_Identity=34.3137254901961, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=26.7267267267267, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42516563, Length=325, Percent_Identity=25.5384615384615, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=34.8101265822785, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=23.0320699708455, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=23.0320699708455, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554428, Length=306, Percent_Identity=29.0849673202614, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=29.2604501607717, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21356223, Length=328, Percent_Identity=25, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 35862; Mature: 35730

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSVDLSQKRILLTGGSGFLGKHVLQQLQVLGVKPEQVRIPRSRTQDLRRWEVCQEVVQ
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHC
GQDIVIHLAAHVGGIGLNQAKPAELFYDNLIMGSQLIHAAYLAGVEKFVCVGTICAYPKF
CCCEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLQAYRQQYGFNGIYLLPVNLYGPMDNFD
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCC
PESSHVIPALIRKVHEAQQRGQNYVEVWGDGSPTREFFYAEDAARGIVMATQLYDGADPL
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEHHHHCCCEEEEEECCCCCCCC
NLGTGEEISIRDLIALICELMEFRGEIRWQTDKPNGQPRRCLDVSKARERMGFVAKVGLR
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EGLQRTIDWYRRHACSGQS
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PSSVDLSQKRILLTGGSGFLGKHVLQQLQVLGVKPEQVRIPRSRTQDLRRWEVCQEVVQ
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHC
GQDIVIHLAAHVGGIGLNQAKPAELFYDNLIMGSQLIHAAYLAGVEKFVCVGTICAYPKF
CCCEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TPVPFKEEDLWNGYPEETNAPYGIAKKALLVQLQAYRQQYGFNGIYLLPVNLYGPMDNFD
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCC
PESSHVIPALIRKVHEAQQRGQNYVEVWGDGSPTREFFYAEDAARGIVMATQLYDGADPL
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEHHHHCCCEEEEEECCCCCCCC
NLGTGEEISIRDLIALICELMEFRGEIRWQTDKPNGQPRRCLDVSKARERMGFVAKVGLR
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EGLQRTIDWYRRHACSGQS
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]