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The map label for this gene is yugF [H]

Identifier: 86608884

GI number: 86608884

Start: 1484645

End: 1485580

Strand: Reverse

Name: yugF [H]

Synonym: CYB_1416

Alternate gene names: 86608884

Gene position: 1485580-1484645 (Counterclockwise)

Preceding gene: 86608885

Following gene: 86608882

Centisome position: 48.76

GC content: 61.86

Gene sequence:

>936_bases
ATGGCTTCGACATCGGCATCGGCCACTCCCATTTCGGCGCAGTGGATCTGGCGGGGGCAGTCAGTTCATTACGTCAAACA
GGGTGAGCAGGGGCAGCCTCTGCTTCTGGTGCACGGCTTTGGAGCCTCCACCGACCACTGGCGCAAGAACATTCCCGAAT
TGGCTCAACACTACCAGGTGTACGCCATCGATTTGCTGGGTTTCGGTCGCTCCGCCAAGCCCAACTGGGACTACCGCGCC
GAAATCTGGCGGGATCAGCTGCGGGACTTTTGCCAACAGGTAATCCGTCGGCCTGTGGTGGGGATCGGCAACTCCCTGGG
CGGCTACGTGGTGCTCAGTTTTGCGGCGGAGTGGCCGGAGTGGGTGCGGGGCGTGGTGCTCTTGAATGGGGCAGGTGGGT
TTTCCACGCTCAAAGGATCCCCGACCGGATGGCGGCAATGGATAGGCGGATTGGTGGGCTGGGGCCTGCGCCAGCGCCTG
GTGTCTTACCTGCTGTTTCAGTATTTGCGGCAGCCGCACGTGATCCGAGCCAAGCTGAAGCAGGTTTACTACGACCCGGC
AGCCGTGACCGATCAGCTGGTCGAGGCCATCCATCGCCCCACCCAGGATCCGGGGGCTGCCGATGTGTTTGTGGCCTTGA
TGCGAGGTGGGCAGAAGGGACGGTATGTAGATGAGCTGCTGTGCAGCTTGGTGCGCCCGCTGTTGTTGATCTGGGGAGAA
CGGGATCCCTGGATGCGGGCTCGGGAACGCTCTAAGCTGTACCGGGCCCACTACCCTCAAGCAGTGGAATACTTCCTGGA
GGCTGGCCACTGCCCCCACGATGAGCGCCCCGAAGAGGTCAATGCCCTGATTCACCGGTGGATCGAGACCGGGATCCCAG
CCACCACTTCTCCCCCCATTTCCAAAGCTGCCCCTGTATCGGAAGTCCATCCCTAG

Upstream 100 bases:

>100_bases
CTAACAGGCCCCCTGCTCTTACGTTGCCCGTAGAGGGTGGGCTTCCCCCCGGAGTAGCTGCAAAGAACCTAGAATTAAGA
AAGGCTTACAAGCAACTCCT

Downstream 100 bases:

>100_bases
GCGGCCCGTCTAGCTTGGGCTATCCAACAATTCCTCCTGGGGATCCCCTTCTAGGCCGATGGTGTACTTAGACCATTCTC
GATGGGAACCACTGCCGACG

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MASTSASATPISAQWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQVYAIDLLGFGRSAKPNWDYRA
EIWRDQLRDFCQQVIRRPVVGIGNSLGGYVVLSFAAEWPEWVRGVVLLNGAGGFSTLKGSPTGWRQWIGGLVGWGLRQRL
VSYLLFQYLRQPHVIRAKLKQVYYDPAAVTDQLVEAIHRPTQDPGAADVFVALMRGGQKGRYVDELLCSLVRPLLLIWGE
RDPWMRARERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIETGIPATTSPPISKAAPVSEVHP

Sequences:

>Translated_311_residues
MASTSASATPISAQWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQVYAIDLLGFGRSAKPNWDYRA
EIWRDQLRDFCQQVIRRPVVGIGNSLGGYVVLSFAAEWPEWVRGVVLLNGAGGFSTLKGSPTGWRQWIGGLVGWGLRQRL
VSYLLFQYLRQPHVIRAKLKQVYYDPAAVTDQLVEAIHRPTQDPGAADVFVALMRGGQKGRYVDELLCSLVRPLLLIWGE
RDPWMRARERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIETGIPATTSPPISKAAPVSEVHP
>Mature_310_residues
ASTSASATPISAQWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQVYAIDLLGFGRSAKPNWDYRAE
IWRDQLRDFCQQVIRRPVVGIGNSLGGYVVLSFAAEWPEWVRGVVLLNGAGGFSTLKGSPTGWRQWIGGLVGWGLRQRLV
SYLLFQYLRQPHVIRAKLKQVYYDPAAVTDQLVEAIHRPTQDPGAADVFVALMRGGQKGRYVDELLCSLVRPLLLIWGER
DPWMRARERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIETGIPATTSPPISKAAPVSEVHP

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=297, Percent_Identity=23.2323232323232, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558492, Length=101, Percent_Identity=34.6534653465347, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32566936, Length=101, Percent_Identity=34.6534653465347, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146278, Length=99, Percent_Identity=34.3434343434343, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586385, Length=277, Percent_Identity=24.5487364620939, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586387, Length=277, Percent_Identity=24.5487364620939, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586391, Length=277, Percent_Identity=24.5487364620939, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586389, Length=277, Percent_Identity=24.5487364620939, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 35177; Mature: 35045

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTSASATPISAQWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQV
CCCCCCCCCCCCCEEEECCCCEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
YAIDLLGFGRSAKPNWDYRAEIWRDQLRDFCQQVIRRPVVGIGNSLGGYVVLSFAAEWPE
HEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHH
WVRGVVLLNGAGGFSTLKGSPTGWRQWIGGLVGWGLRQRLVSYLLFQYLRQPHVIRAKLK
HHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVYYDPAAVTDQLVEAIHRPTQDPGAADVFVALMRGGQKGRYVDELLCSLVRPLLLIWGE
HHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
RDPWMRARERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIETGIPATTSPPI
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SKAAPVSEVHP
CCCCCCCCCCC
>Mature Secondary Structure 
ASTSASATPISAQWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQV
CCCCCCCCCCCCEEEECCCCEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
YAIDLLGFGRSAKPNWDYRAEIWRDQLRDFCQQVIRRPVVGIGNSLGGYVVLSFAAEWPE
HEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHH
WVRGVVLLNGAGGFSTLKGSPTGWRQWIGGLVGWGLRQRLVSYLLFQYLRQPHVIRAKLK
HHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVYYDPAAVTDQLVEAIHRPTQDPGAADVFVALMRGGQKGRYVDELLCSLVRPLLLIWGE
HHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
RDPWMRARERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIETGIPATTSPPI
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SKAAPVSEVHP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]