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The map label for this gene is potF [H]

Identifier: 86607999

GI number: 86607999

Start: 516914

End: 518011

Strand: Direct

Name: potF [H]

Synonym: CYB_0505

Alternate gene names: 86607999

Gene position: 516914-518011 (Clockwise)

Preceding gene: 86607998

Following gene: 86608000

Centisome position: 16.97

GC content: 55.74

Gene sequence:

>1098_bases
ATGTTTTCAACTTTTCACAGTCGGAGAAATCTCATGAAAAAGTGGATTGCCTCGGCTACAACCCTGCTCTGCTGTGTGCT
TGTCCTGGTTTTGTTTTGGCCCCATGTTACCTCTGCCCAGAGGCAGACCCTCAATCTCTACATCTGGTCAGAATACATCG
ATCCCGACATCCTCACCGCCTTTGAAAAAGCCACCAATAGCCGCGTGCTGGTCAGCGTGTACGAATCCAACGAAGACATG
GTGGCCAAACTGCGGGGGGGAGGCACCAGCCAGTACGACATTGTTGTTCCCACCGACTACATTGTGCCCAACATGTTGGA
GCTGGGACTGCTGCAGCCTCTCAACAAAGAATTGATCCCCAACCTAAGCAACCTGGGCGAAAAATTTGTCGGCCTGCCCT
TTGATCCAGAAAACACCTACACCGCCGCCTATCAGTGGGGCACCACCGGCATTGGCTACCGCAAAGACCGCCTGCCCGAA
AACTTTGAACGCTCTTGGGGATTGATTTTCGACCCGCAGCAGCAGTATGGCCCCTTTGTGATGATCGACGAGATGCGCTC
CATGATCGGCGCTGCTGCCGTTTACCTGGGCTTCGATATGAACACCACCGTTCGCTCCGAACTGCAGCAGATCCAGCAGC
TTTTGATCGACACCAAGCGCCGCAGTGCCGGCCTAATTGGGGGCGTGGGGGGCAAAAACCAACTGGTTTCTGGAACGGCC
AATGTAGCCATCGTCTACAGCGGCGATGCCCTGCAAGCTGCCGAAGAAAACCCCAACATCGGCTATTTTGTCCCCCGCGA
GGGTGCCCACATTTGGCTGGATGTAATGGCCATTCCCGCCCAGGCCCCCAACCCCGAGCTGGCCAATCGCTTCATCAATT
TCATCCTGGATGCTGAAGTCGGCGCTCAACTGAGCAACCACAACCGCTTCGCCACCCCCAACGCTGCAGCTCTGCCCATG
ATCAACCCAGAAGATCGGCAAAACCCGGCCATCTACCCTGACGAAGAGGCCCTTGCCAAACTCACCTACCTTAAAGTGCT
GTCAGGGGAAGAGCTGCGCCTCATCGACGCCCTCTGGACAACCGTGAAAAGCAGCTAG

Upstream 100 bases:

>100_bases
GAGTACCCGAGCCTCAACCGGAGTTTGCGGGGCTTGGCGATTGCCCAAGGCGTGCCCCCTAGCCGCGGGTGTAGCCGGCT
GGGGGATGAAAGCAGATACG

Downstream 100 bases:

>100_bases
GGATCCCTTGTTAGCTCTCGACATAAAACTTGACACTTTTTAATATCTGAGCCACATTAAAGATGTCACCCGTTGATGGA
GCTAGAACCATGGAAAACCA

Product: polyamine transporter, periplasmic polyamine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MFSTFHSRRNLMKKWIASATTLLCCVLVLVLFWPHVTSAQRQTLNLYIWSEYIDPDILTAFEKATNSRVLVSVYESNEDM
VAKLRGGGTSQYDIVVPTDYIVPNMLELGLLQPLNKELIPNLSNLGEKFVGLPFDPENTYTAAYQWGTTGIGYRKDRLPE
NFERSWGLIFDPQQQYGPFVMIDEMRSMIGAAAVYLGFDMNTTVRSELQQIQQLLIDTKRRSAGLIGGVGGKNQLVSGTA
NVAIVYSGDALQAAEENPNIGYFVPREGAHIWLDVMAIPAQAPNPELANRFINFILDAEVGAQLSNHNRFATPNAAALPM
INPEDRQNPAIYPDEEALAKLTYLKVLSGEELRLIDALWTTVKSS

Sequences:

>Translated_365_residues
MFSTFHSRRNLMKKWIASATTLLCCVLVLVLFWPHVTSAQRQTLNLYIWSEYIDPDILTAFEKATNSRVLVSVYESNEDM
VAKLRGGGTSQYDIVVPTDYIVPNMLELGLLQPLNKELIPNLSNLGEKFVGLPFDPENTYTAAYQWGTTGIGYRKDRLPE
NFERSWGLIFDPQQQYGPFVMIDEMRSMIGAAAVYLGFDMNTTVRSELQQIQQLLIDTKRRSAGLIGGVGGKNQLVSGTA
NVAIVYSGDALQAAEENPNIGYFVPREGAHIWLDVMAIPAQAPNPELANRFINFILDAEVGAQLSNHNRFATPNAAALPM
INPEDRQNPAIYPDEEALAKLTYLKVLSGEELRLIDALWTTVKSS
>Mature_365_residues
MFSTFHSRRNLMKKWIASATTLLCCVLVLVLFWPHVTSAQRQTLNLYIWSEYIDPDILTAFEKATNSRVLVSVYESNEDM
VAKLRGGGTSQYDIVVPTDYIVPNMLELGLLQPLNKELIPNLSNLGEKFVGLPFDPENTYTAAYQWGTTGIGYRKDRLPE
NFERSWGLIFDPQQQYGPFVMIDEMRSMIGAAAVYLGFDMNTTVRSELQQIQQLLIDTKRRSAGLIGGVGGKNQLVSGTA
NVAIVYSGDALQAAEENPNIGYFVPREGAHIWLDVMAIPAQAPNPELANRFINFILDAEVGAQLSNHNRFATPNAAALPM
INPEDRQNPAIYPDEEALAKLTYLKVLSGEELRLIDALWTTVKSS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=371, Percent_Identity=34.5013477088949, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1787367, Length=297, Percent_Identity=34.3434343434343, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40696; Mature: 40696

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSTFHSRRNLMKKWIASATTLLCCVLVLVLFWPHVTSAQRQTLNLYIWSEYIDPDILTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHH
FEKATNSRVLVSVYESNEDMVAKLRGGGTSQYDIVVPTDYIVPNMLELGLLQPLNKELIP
HHHCCCCEEEEEEEECCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHCC
NLSNLGEKFVGLPFDPENTYTAAYQWGTTGIGYRKDRLPENFERSWGLIFDPQQQYGPFV
CHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCHHHCCCEEECCHHHCCCEE
MIDEMRSMIGAAAVYLGFDMNTTVRSELQQIQQLLIDTKRRSAGLIGGVGGKNQLVSGTA
EHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCC
NVAIVYSGDALQAAEENPNIGYFVPREGAHIWLDVMAIPAQAPNPELANRFINFILDAEV
CEEEEECCCHHHHHCCCCCCCEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
GAQLSNHNRFATPNAAALPMINPEDRQNPAIYPDEEALAKLTYLKVLSGEELRLIDALWT
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
TVKSS
HHCCC
>Mature Secondary Structure
MFSTFHSRRNLMKKWIASATTLLCCVLVLVLFWPHVTSAQRQTLNLYIWSEYIDPDILTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHH
FEKATNSRVLVSVYESNEDMVAKLRGGGTSQYDIVVPTDYIVPNMLELGLLQPLNKELIP
HHHCCCCEEEEEEEECCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHCC
NLSNLGEKFVGLPFDPENTYTAAYQWGTTGIGYRKDRLPENFERSWGLIFDPQQQYGPFV
CHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCHHHCCCEEECCHHHCCCEE
MIDEMRSMIGAAAVYLGFDMNTTVRSELQQIQQLLIDTKRRSAGLIGGVGGKNQLVSGTA
EHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCC
NVAIVYSGDALQAAEENPNIGYFVPREGAHIWLDVMAIPAQAPNPELANRFINFILDAEV
CEEEEECCCHHHHHCCCCCCCEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
GAQLSNHNRFATPNAAALPMINPEDRQNPAIYPDEEALAKLTYLKVLSGEELRLIDALWT
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
TVKSS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]