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The map label for this gene is birA [H]

Identifier: 86607952

GI number: 86607952

Start: 462170

End: 463003

Strand: Direct

Name: birA [H]

Synonym: CYB_0457

Alternate gene names: 86607952

Gene position: 462170-463003 (Clockwise)

Preceding gene: 86607951

Following gene: 86607953

Centisome position: 15.17

GC content: 61.63

Gene sequence:

>834_bases
ATGCTTTCTCCCTCGCGGCTGGGATCCCAGGCGGGCCGGTGGGGCCGCCATCTGCTGCAAGTGGCGGTGACCGATTCCAC
CAATCGGCTGCTCTTGGACTGGGGCCGTCGGTTCCCACAGCCGCTGCCAGTGGGCACAGTTTTGGTCAGCACCCGCCAAC
GGGCTGGGCAAGGTCAACATGGGCGGGTGTGGCACTCGCAGCCGGGGGGACTATACCTGTCCGTTTGGCTGGGATCCCCG
CCTTTGCATTACTCGCTGCTGGAACTGACCCTGGCCTTGGGCTGGGGCATTGCGGGCCGTTTACGGCAAAACCTTGGGAT
CCCGCTGCGGCTGAAATGGCCCAACGATTTGGTGGTGGTGGACGAGCAGCGCCCGGATTCTCTGCTCAAGCTGGGGGGGA
TCCTGGTGCAAAGCCGCTTTGGGGGGAGCGGGCAACTGCTGGGGTTGGTGGCCGGTGTGGGGGTGAATGTCAACAACCCC
GTTCCCATTGGGGCAATCACCTTAGCCCAAATTTTGGGATCCCCTCTGCACCGCTCACTCGACTGCACCGAGATTGCCGG
CTGGGTTCTGCAGGGGATGGAGCAGGGATTCCACGTCTGGTTGCAAGGGGGATTTGCCCCCGTTCGCTCAGAGTACGAAG
CCTGGATGGTTAGGCCACAAATTCAATTTCAATGGCCGGAAACAAGCCCCACGATCTTGGGCCTTGCCGAGGATGGGAGG
ATGCGAGTACAAACACGGGGGGAGCGACATTGGCAACAGCAGCCATCCATTCTGACGCTTACACCCGACCAAGTTCGCTT
GAGCTATCACATGCCGGCGGGCGATAGCCTGTAA

Upstream 100 bases:

>100_bases
CTCGGGCAATGATCTGCTGGACATGTTGCGCTACCAAAACCTCAAGCGGCTGGCCCAGCGTCCGGCGGAAGACATTGTGT
TGAGCTAATCCCGGGTAGAG

Downstream 100 bases:

>100_bases
TTTCTCTTGTGTTTTTTATGTGGCCTGTGTTGAACTCGGTGATGAAGCTTTTGGTTATGGCAGCGCGCCTTTTCTCCCCC
CGAGCTCGGCGGCTGCGCTT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLSPSRLGSQAGRWGRHLLQVAVTDSTNRLLLDWGRRFPQPLPVGTVLVSTRQRAGQGQHGRVWHSQPGGLYLSVWLGSP
PLHYSLLELTLALGWGIAGRLRQNLGIPLRLKWPNDLVVVDEQRPDSLLKLGGILVQSRFGGSGQLLGLVAGVGVNVNNP
VPIGAITLAQILGSPLHRSLDCTEIAGWVLQGMEQGFHVWLQGGFAPVRSEYEAWMVRPQIQFQWPETSPTILGLAEDGR
MRVQTRGERHWQQQPSILTLTPDQVRLSYHMPAGDSL

Sequences:

>Translated_277_residues
MLSPSRLGSQAGRWGRHLLQVAVTDSTNRLLLDWGRRFPQPLPVGTVLVSTRQRAGQGQHGRVWHSQPGGLYLSVWLGSP
PLHYSLLELTLALGWGIAGRLRQNLGIPLRLKWPNDLVVVDEQRPDSLLKLGGILVQSRFGGSGQLLGLVAGVGVNVNNP
VPIGAITLAQILGSPLHRSLDCTEIAGWVLQGMEQGFHVWLQGGFAPVRSEYEAWMVRPQIQFQWPETSPTILGLAEDGR
MRVQTRGERHWQQQPSILTLTPDQVRLSYHMPAGDSL
>Mature_277_residues
MLSPSRLGSQAGRWGRHLLQVAVTDSTNRLLLDWGRRFPQPLPVGTVLVSTRQRAGQGQHGRVWHSQPGGLYLSVWLGSP
PLHYSLLELTLALGWGIAGRLRQNLGIPLRLKWPNDLVVVDEQRPDSLLKLGGILVQSRFGGSGQLLGLVAGVGVNVNNP
VPIGAITLAQILGSPLHRSLDCTEIAGWVLQGMEQGFHVWLQGGFAPVRSEYEAWMVRPQIQFQWPETSPTILGLAEDGR
MRVQTRGERHWQQQPSILTLTPDQVRLSYHMPAGDSL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=169, Percent_Identity=33.7278106508876, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790408, Length=198, Percent_Identity=32.3232323232323, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30573; Mature: 30573

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPSRLGSQAGRWGRHLLQVAVTDSTNRLLLDWGRRFPQPLPVGTVLVSTRQRAGQGQH
CCCHHHHHHHHHHHHHEEEEEEEECCCCEEEEEHHHHCCCCCCCCCEEEECHHHCCCCCC
GRVWHSQPGGLYLSVWLGSPPLHYSLLELTLALGWGIAGRLRQNLGIPLRLKWPNDLVVV
CCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCEEEE
DEQRPDSLLKLGGILVQSRFGGSGQLLGLVAGVGVNVNNPVPIGAITLAQILGSPLHRSL
ECCCCHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHCCC
DCTEIAGWVLQGMEQGFHVWLQGGFAPVRSEYEAWMVRPQIQFQWPETSPTILGLAEDGR
CHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCEEEEECCEEEEECCCCCCEEEEEECCCC
MRVQTRGERHWQQQPSILTLTPDQVRLSYHMPAGDSL
EEEEECCHHHHHCCCCEEEECCCCEEEEEECCCCCCC
>Mature Secondary Structure
MLSPSRLGSQAGRWGRHLLQVAVTDSTNRLLLDWGRRFPQPLPVGTVLVSTRQRAGQGQH
CCCHHHHHHHHHHHHHEEEEEEEECCCCEEEEEHHHHCCCCCCCCCEEEECHHHCCCCCC
GRVWHSQPGGLYLSVWLGSPPLHYSLLELTLALGWGIAGRLRQNLGIPLRLKWPNDLVVV
CCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCEEEE
DEQRPDSLLKLGGILVQSRFGGSGQLLGLVAGVGVNVNNPVPIGAITLAQILGSPLHRSL
ECCCCHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHCCC
DCTEIAGWVLQGMEQGFHVWLQGGFAPVRSEYEAWMVRPQIQFQWPETSPTILGLAEDGR
CHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCEEEEECCEEEEECCCCCCEEEEEECCCC
MRVQTRGERHWQQQPSILTLTPDQVRLSYHMPAGDSL
EEEEECCHHHHHCCCCEEEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]