Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 86606590

GI number: 86606590

Start: 1954758

End: 1957040

Strand: Reverse

Name: spoT [H]

Synonym: CYA_1944

Alternate gene names: 86606590

Gene position: 1957040-1954758 (Counterclockwise)

Preceding gene: 86606591

Following gene: 86606589

Centisome position: 66.73

GC content: 58.43

Gene sequence:

>2283_bases
ATGGGTCAGCCTGCTGTTGCCGCCAGCTTAGAGGGAGGCTTTGACCTAAATAGCCTGGATTTTGAGGTGCCGGGATGGCT
GCGGGCTTTGCTTTCAGGCGCTGAAGATCTCGGCGATGGGCTGCGATCCTACACGGCGGAAGAGGCAGATTTGCTGCGCC
GGGCCTTCGAGTTTGCCTACTGTTTGCACAAGGGCCAAAAGCGGAAATCAGGGGATCCCTACATCGCCCATCCGGTGGCC
GTGGCCAGCCTGTTGCGGGAGTTGGGGGGTGACGCCGTTACCGTAGCTGCTGGCCTTCTGCACGATGTTGTCGAAGATAC
GGAAGTTACCCTGGAGGCCCTAGAGGCCGAATTTGGCCCTGAGGTTCGCCTGCTGGTGGAGGGGGTAACCAAGCTTTCTC
AGTTTAATTTCTCCAGCAAAACGGAGCAGCAAGCAGAAAACTTCCGCCGCATGTTTGTGGCCATGGCCAAGGATATCCGG
GTCATTGTGGTCAAGTTGGCGGATCGCTTGCACAACATGCGCACGCTGCAATATCTGCCCCCCCACAAGCAAAAACAGAT
TGCAGCCGAAACTATGGAGATCTTCGCCCCCTTGGCCAACCGGCTGGGGATCTGGCATTTCAAGTGGGAACTGGAAGACT
TGGCCTTTAAGTACCTGGACTACGAAGCCTATCGCCAGATTCAAGAGCTGGTCAACGCCAAGCGTAGCGAGCGAGAGGCA
GAGATCCAAGCGTTTATTGAAGAGCTGCGGAAGCACCTCATTCAAGGGGGGCTAGAGCACTTTGAGATTAGCGGGCGGCC
CAAGCACCTGTACGGCATCTACCGCAAGATGCAGCAGCAGCGCAAAGAATTTCACGAGATCTACGATCTCCTGGCCGTGC
GGGTTATCGTGCAAACCGTGAGCGAATGCTACCGGGTGCTGGCGGTCGTTCACAACTGTTTTCGCCCCATTCCGGGTCGT
TTCAAAGACTACATTGGCTTGCCCAAGGCCAACCGCTACCAATCTTTGCATACGGCTGTGATCGGCCTACAGGGCCGACC
GGTGGAGGTGCAGATCCGCACCGAAGAGATGCACCGCGTGGCCGAATACGGCATTGCCGCCCACTGGAAGTACAAAGAAG
CCGGCAGCACCCCCGTAAAGCCCGAGGAAGAGCGCTTTACCTGGCTGCGGCAACTGTTGGAGTGGCAAAACGACCTCAAG
GACGACAAAGAATACCTGGACACCATTCGCGAGAACCTATTTGAGAGCGAGGTTTACGTCTTTACGCCCAAAGGAGACTT
GCTGGCCCTGCCCCAGGGATCCTGCCCGGTGGACTTTGCCTATCGCATTCACTCTGAAATTGGGGATCACTGTGCCGGGG
CGCGGGTGAACAACAAGATCGTGCCCTTGAATACCCGCCTGCGCAATGGCGACATTGTCCAAATCATCACCCACAAGAAC
GCCCACCCCAGCCTGGACTGGATCAATTTTGTAGCCACCAGCTCGGCCCGCAACCGCATTCGCCAATGGTTTAAGCGCTG
CAATCGCGAGCAAAACATCGCCCGGGGCCGGGACTTGCTGGAGCGGGAGCTGGGCAAAACGGGGTTCGATGCGCTGCTCA
AGTCGGAGCGGATGCAAAAGGTGGCCGAGAAGCTCAACTACACCAGCGTGGACGACCTGCTGGCGGCGCTGGGCTATGGA
GAGGTCACCCCCACGCAGGTGCTCCATCGGCTGCAGGAGGTGAGCAAGCCGGCAGTCTCTCCCCCGCCAGAGGAGGGATC
CCTTGAGGAAGCCAAGCTGCTGCTGCCGCGGGCCAATGGGAGCCGAGGTTCCAGCCGATCCCAATCTCCCATCCTGGGGG
TGGAGGGTCTGAAACACCAGTTGGCCAAATGTTGCAACCCGCTGCCAGGGGATCCCATCCTGGGCGTGGTTACCCGCTTC
AAGGGTATCACCATTCATCATCGCGATTGTCCGCGGGTGGCTCAGGTTCCGGGGGAGCGGCTGGTGCCGGTGCGCTGGAA
CGAGGAGCAATTACGGCAGAGCACCCAGATCTACCCTGTGGAACTGCGCCTGGAGGTCATTGACCGCGTTGGCGTGCTGC
GGGACATCCTTTCCCGTCTTTCGGATCAGAGCATTAATGTGCGCAATGCCAGGGTGCAGACCTTTGCCAACCGTACGGCT
CTGATCGATCTCTGTATTGATGTGCGGGACAAAAAACAGCTCGACCAAGTTCAGAGTCAATTGCGCCAGCTCTCGGATGT
GCTGGTGTTGCGCTCCCGCCACTACCAGAACGGATCCCGCTGA

Upstream 100 bases:

>100_bases
TCGCAGCGCTTTTTGCCTTTAACCTGCCTGCGGACGGGTAAAGTTGGGGATAGGCTGTAAGAAACTCCAGGGAGTAAACC
ATCCTCCTTGCTAGATTCCC

Downstream 100 bases:

>100_bases
AGAACTTCTAGGGTCTTTTTTTCGCTGGTTCGAGAGGAGGCGCCCATATTAGGATGATTGAGATGGGTAACCAATCTGAG
CGGAGGATCCCGGCATGTCA

Product: RelA/SpoT family protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MGQPAVAASLEGGFDLNSLDFEVPGWLRALLSGAEDLGDGLRSYTAEEADLLRRAFEFAYCLHKGQKRKSGDPYIAHPVA
VASLLRELGGDAVTVAAGLLHDVVEDTEVTLEALEAEFGPEVRLLVEGVTKLSQFNFSSKTEQQAENFRRMFVAMAKDIR
VIVVKLADRLHNMRTLQYLPPHKQKQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRQIQELVNAKRSEREA
EIQAFIEELRKHLIQGGLEHFEISGRPKHLYGIYRKMQQQRKEFHEIYDLLAVRVIVQTVSECYRVLAVVHNCFRPIPGR
FKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRVAEYGIAAHWKYKEAGSTPVKPEEERFTWLRQLLEWQNDLK
DDKEYLDTIRENLFESEVYVFTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLNTRLRNGDIVQIITHKN
AHPSLDWINFVATSSARNRIRQWFKRCNREQNIARGRDLLERELGKTGFDALLKSERMQKVAEKLNYTSVDDLLAALGYG
EVTPTQVLHRLQEVSKPAVSPPPEEGSLEEAKLLLPRANGSRGSSRSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRF
KGITIHHRDCPRVAQVPGERLVPVRWNEEQLRQSTQIYPVELRLEVIDRVGVLRDILSRLSDQSINVRNARVQTFANRTA
LIDLCIDVRDKKQLDQVQSQLRQLSDVLVLRSRHYQNGSR

Sequences:

>Translated_760_residues
MGQPAVAASLEGGFDLNSLDFEVPGWLRALLSGAEDLGDGLRSYTAEEADLLRRAFEFAYCLHKGQKRKSGDPYIAHPVA
VASLLRELGGDAVTVAAGLLHDVVEDTEVTLEALEAEFGPEVRLLVEGVTKLSQFNFSSKTEQQAENFRRMFVAMAKDIR
VIVVKLADRLHNMRTLQYLPPHKQKQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRQIQELVNAKRSEREA
EIQAFIEELRKHLIQGGLEHFEISGRPKHLYGIYRKMQQQRKEFHEIYDLLAVRVIVQTVSECYRVLAVVHNCFRPIPGR
FKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRVAEYGIAAHWKYKEAGSTPVKPEEERFTWLRQLLEWQNDLK
DDKEYLDTIRENLFESEVYVFTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLNTRLRNGDIVQIITHKN
AHPSLDWINFVATSSARNRIRQWFKRCNREQNIARGRDLLERELGKTGFDALLKSERMQKVAEKLNYTSVDDLLAALGYG
EVTPTQVLHRLQEVSKPAVSPPPEEGSLEEAKLLLPRANGSRGSSRSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRF
KGITIHHRDCPRVAQVPGERLVPVRWNEEQLRQSTQIYPVELRLEVIDRVGVLRDILSRLSDQSINVRNARVQTFANRTA
LIDLCIDVRDKKQLDQVQSQLRQLSDVLVLRSRHYQNGSR
>Mature_759_residues
GQPAVAASLEGGFDLNSLDFEVPGWLRALLSGAEDLGDGLRSYTAEEADLLRRAFEFAYCLHKGQKRKSGDPYIAHPVAV
ASLLRELGGDAVTVAAGLLHDVVEDTEVTLEALEAEFGPEVRLLVEGVTKLSQFNFSSKTEQQAENFRRMFVAMAKDIRV
IVVKLADRLHNMRTLQYLPPHKQKQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRQIQELVNAKRSEREAE
IQAFIEELRKHLIQGGLEHFEISGRPKHLYGIYRKMQQQRKEFHEIYDLLAVRVIVQTVSECYRVLAVVHNCFRPIPGRF
KDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRVAEYGIAAHWKYKEAGSTPVKPEEERFTWLRQLLEWQNDLKD
DKEYLDTIRENLFESEVYVFTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLNTRLRNGDIVQIITHKNA
HPSLDWINFVATSSARNRIRQWFKRCNREQNIARGRDLLERELGKTGFDALLKSERMQKVAEKLNYTSVDDLLAALGYGE
VTPTQVLHRLQEVSKPAVSPPPEEGSLEEAKLLLPRANGSRGSSRSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRFK
GITIHHRDCPRVAQVPGERLVPVRWNEEQLRQSTQIYPVELRLEVIDRVGVLRDILSRLSDQSINVRNARVQTFANRTAL
IDLCIDVRDKKQLDQVQSQLRQLSDVLVLRSRHYQNGSR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=140, Percent_Identity=42.1428571428571, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1790082, Length=724, Percent_Identity=41.4364640883978, Blast_Score=560, Evalue=1e-160,
Organism=Escherichia coli, GI1789147, Length=719, Percent_Identity=37.413073713491, Blast_Score=497, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI25143118, Length=135, Percent_Identity=38.5185185185185, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650996, Length=131, Percent_Identity=35.1145038167939, Blast_Score=70, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86690; Mature: 86559

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQPAVAASLEGGFDLNSLDFEVPGWLRALLSGAEDLGDGLRSYTAEEADLLRRAFEFAY
CCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHH
CLHKGQKRKSGDPYIAHPVAVASLLRELGGDAVTVAAGLLHDVVEDTEVTLEALEAEFGP
HHHCCCCCCCCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVRLLVEGVTKLSQFNFSSKTEQQAENFRRMFVAMAKDIRVIVVKLADRLHNMRTLQYLP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PHKQKQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRQIQELVNAKRSEREA
CCHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFIEELRKHLIQGGLEHFEISGRPKHLYGIYRKMQQQRKEFHEIYDLLAVRVIVQTV
HHHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SECYRVLAVVHNCFRPIPGRFKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRV
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHEECCCCCEEEEEEHHHHHHH
AEYGIAAHWKYKEAGSTPVKPEEERFTWLRQLLEWQNDLKDDKEYLDTIRENLFESEVYV
HHHCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
FTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLNTRLRNGDIVQIITHKN
ECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECCCCEEEEEECCC
AHPSLDWINFVATSSARNRIRQWFKRCNREQNIARGRDLLERELGKTGFDALLKSERMQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAEKLNYTSVDDLLAALGYGEVTPTQVLHRLQEVSKPAVSPPPEEGSLEEAKLLLPRANG
HHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCC
SRGSSRSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRFKGITIHHRDCPRVAQVPGER
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHCCCCC
LVPVRWNEEQLRQSTQIYPVELRLEVIDRVGVLRDILSRLSDQSINVRNARVQTFANRTA
EEECCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LIDLCIDVRDKKQLDQVQSQLRQLSDVLVLRSRHYQNGSR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GQPAVAASLEGGFDLNSLDFEVPGWLRALLSGAEDLGDGLRSYTAEEADLLRRAFEFAY
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHH
CLHKGQKRKSGDPYIAHPVAVASLLRELGGDAVTVAAGLLHDVVEDTEVTLEALEAEFGP
HHHCCCCCCCCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVRLLVEGVTKLSQFNFSSKTEQQAENFRRMFVAMAKDIRVIVVKLADRLHNMRTLQYLP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PHKQKQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRQIQELVNAKRSEREA
CCHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFIEELRKHLIQGGLEHFEISGRPKHLYGIYRKMQQQRKEFHEIYDLLAVRVIVQTV
HHHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SECYRVLAVVHNCFRPIPGRFKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRV
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHEECCCCCEEEEEEHHHHHHH
AEYGIAAHWKYKEAGSTPVKPEEERFTWLRQLLEWQNDLKDDKEYLDTIRENLFESEVYV
HHHCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
FTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLNTRLRNGDIVQIITHKN
ECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECCCCEEEEEECCC
AHPSLDWINFVATSSARNRIRQWFKRCNREQNIARGRDLLERELGKTGFDALLKSERMQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAEKLNYTSVDDLLAALGYGEVTPTQVLHRLQEVSKPAVSPPPEEGSLEEAKLLLPRANG
HHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCC
SRGSSRSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRFKGITIHHRDCPRVAQVPGER
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHCCCCC
LVPVRWNEEQLRQSTQIYPVELRLEVIDRVGVLRDILSRLSDQSINVRNARVQTFANRTA
EEECCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LIDLCIDVRDKKQLDQVQSQLRQLSDVLVLRSRHYQNGSR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]