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The map label for this gene is cobM

Identifier: 86606171

GI number: 86606171

Start: 1511999

End: 1512763

Strand: Reverse

Name: cobM

Synonym: CYA_1504

Alternate gene names: 86606171

Gene position: 1512763-1511999 (Counterclockwise)

Preceding gene: 86606172

Following gene: 86606170

Centisome position: 51.58

GC content: 66.8

Gene sequence:

>765_bases
GTGCACATTGTCGGCGCCGGCCCTGGGGATCCCGAGCTGATTACCCTGCGCGGCCAGAGAGTACTCTCAGAAGCAGATGT
AGTATTCTATACCGGCTCTTTGGTGCCGGAGGGGCTGCTGGCCCATTGTCGCCCCGACGTGGAGGCCATCGACACGCGCT
CCCTCACCTTGGAAACGCTGCTGCCCCAGATGGTGGAGCGGGTTAAGGCGGGGCGGCGGGTGGTGCGGCTGCAGGATGGG
GATCCGTCTCTTTACGGCGCTCTGCACGAGTTGCTGATTCGGCTGGCGGAGGCCGGGATCCCCTTTGAGATCGTGCCGGG
GGTGAGCGCCTTTCAACTGGCAGCCGCCCGTTTGCAGGTGGAGCTAACGGTGCCGGAGCTGGTGCAGACGGTCATCCTCA
CCCGCGCCAGCGGTCGGACGCGCGTGCCGGAAGCAGAAGCCCTGGCCAGCCTAGCGGCCTGCCGCGCCTCTTTGTGTCTG
TATCTGAGCGCCCGCCATGTGGAACAGGCCCAGGCGGATCTAATGACCCACTATCCCGCCGATACCCCTGTGGCCATCTG
CTACCGTCTGGGCTGGCCGGACGAGCGGATCTGGCTGGGATCCCTGGCGGAGATGGCGGCCCTCACCCGGCAAGCCGGCC
TAGAGCGCACCGTGCTCTACCTCATCAGTCCCGCCCTACGGGGCTTGGCAGGAGCCGGCTCGGCCCGCTCGCGGCTCTAC
AGCCCTGACCACGCCCACCTCTTCCGTCCCAAACGGCACCGTTAG

Upstream 100 bases:

>100_bases
AAGGGACAGAGCCTGCAGGGGATCCCATCCTCGGGGGTAGCCCTCTGCTGCTAGGATGGGAAGAAACCCGATGAGCCAAC
CGACTTCGTCCTACTGGCCG

Downstream 100 bases:

>100_bases
ACTCTACTGCAACTGGCTTTTTTAGGACTTGCCTTGGAGGCCCCCGGCCTGTCGCAGCTACTTTGGGCACCTGGGCGTGT
TGCCCTGCTGGTCAGCGAAA

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MHIVGAGPGDPELITLRGQRVLSEADVVFYTGSLVPEGLLAHCRPDVEAIDTRSLTLETLLPQMVERVKAGRRVVRLQDG
DPSLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQVELTVPELVQTVILTRASGRTRVPEAEALASLAACRASLCL
YLSARHVEQAQADLMTHYPADTPVAICYRLGWPDERIWLGSLAEMAALTRQAGLERTVLYLISPALRGLAGAGSARSRLY
SPDHAHLFRPKRHR

Sequences:

>Translated_254_residues
MHIVGAGPGDPELITLRGQRVLSEADVVFYTGSLVPEGLLAHCRPDVEAIDTRSLTLETLLPQMVERVKAGRRVVRLQDG
DPSLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQVELTVPELVQTVILTRASGRTRVPEAEALASLAACRASLCL
YLSARHVEQAQADLMTHYPADTPVAICYRLGWPDERIWLGSLAEMAALTRQAGLERTVLYLISPALRGLAGAGSARSRLY
SPDHAHLFRPKRHR
>Mature_254_residues
MHIVGAGPGDPELITLRGQRVLSEADVVFYTGSLVPEGLLAHCRPDVEAIDTRSLTLETLLPQMVERVKAGRRVVRLQDG
DPSLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQVELTVPELVQTVILTRASGRTRVPEAEALASLAACRASLCL
YLSARHVEQAQADLMTHYPADTPVAICYRLGWPDERIWLGSLAEMAALTRQAGLERTVLYLISPALRGLAGAGSARSRLY
SPDHAHLFRPKRHR

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=216, Percent_Identity=31.9444444444444, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27690; Mature: 27690

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVGAGPGDPELITLRGQRVLSEADVVFYTGSLVPEGLLAHCRPDVEAIDTRSLTLETL
CEEECCCCCCCCEEEEECCHHHCCCCEEEEECCCCHHHHHHHCCCCHHHHCCCHHHHHHH
LPQMVERVKAGRRVVRLQDGDPSLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQV
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHEE
ELTVPELVQTVILTRASGRTRVPEAEALASLAACRASLCLYLSARHVEQAQADLMTHYPA
EECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DTPVAICYRLGWPDERIWLGSLAEMAALTRQAGLERTVLYLISPALRGLAGAGSARSRLY
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCC
SPDHAHLFRPKRHR
CCCHHHHCCCCCCC
>Mature Secondary Structure
MHIVGAGPGDPELITLRGQRVLSEADVVFYTGSLVPEGLLAHCRPDVEAIDTRSLTLETL
CEEECCCCCCCCEEEEECCHHHCCCCEEEEECCCCHHHHHHHCCCCHHHHCCCHHHHHHH
LPQMVERVKAGRRVVRLQDGDPSLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQV
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHEE
ELTVPELVQTVILTRASGRTRVPEAEALASLAACRASLCLYLSARHVEQAQADLMTHYPA
EECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DTPVAICYRLGWPDERIWLGSLAEMAALTRQAGLERTVLYLISPALRGLAGAGSARSRLY
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCC
SPDHAHLFRPKRHR
CCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]