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The map label for this gene is cca [H]

Identifier: 86605729

GI number: 86605729

Start: 1051100

End: 1052437

Strand: Reverse

Name: cca [H]

Synonym: CYA_1032

Alternate gene names: 86605729

Gene position: 1052437-1051100 (Counterclockwise)

Preceding gene: 86605730

Following gene: 86605727

Centisome position: 35.89

GC content: 67.94

Gene sequence:

>1338_bases
ATGAGCGAAGCCCTGCTGAGCAAGTGGCTGCTGACCTATTTCCGCCAACGGCTGCCCTTTCCCAAAGAGTGGCTGCCCCA
GGGGAGCTACTGGGTGGGGGGATCCCTGCGGGATGCCTATCTGGCTTGGCTGCGGGGCCAGGCTGGAGACTCCAAGGGGC
CGGTGGATCTGGATTTAATGTGTCCGGCGGATCCCGTGGGGTGGGCGGCGGGGGTGGCGCGGCGGCTGAAGGCCGGCTTT
GTGGTGCTGGATGCGGAGCGACAGATTGCCCGCATCGTGCTGGGCCAAGGAGGGGCTTCCACCCCCACAGCGGCGACGGT
GGACGCGGCCCTGCAGGTGGGCCCCACCCCGGAGGCAGATCTGCGGCAGCGGGACTTCACCTGCAATGCCCTGGCTCTGG
AACTGCAGGAGGGGCACCTGTTGGATCCCACGGGGGGGCGGGCAGACATTGAGCAGAGGTGTCTGCGCATGGTTCACCCC
GACAACCTCAGTGCCGATCCGGTGCGGCTGTTGCGGGCCTATCGCCAGGCAGCTCAACTGGGGTTTGCGCTGGATCCGGC
CACGGCAGAGGCGATTCGTCAGCGGGCCCCCCTTTTGGCCAGGGTGGCGGCAGAGCGGGTGCGGGCCGAGGTGGTGACCT
TGTTGGAGACGGGGATCCCCGGCTTGGCCCACCTGCAGGCAGCGGTGGCGGCAGGTCTGCTAGCGGCTTGGCTGCCCCGC
CTAGCGCCCCAGAGCCCCGGCTTTGCCCAGGCCCAAGCGGTGTGGCAATGGGCCGAGATCTGGCAGCTTCAGTATCCCCG
CACTCTGGCGGAGCTGGCCCAGCCTTTGGCGGATCGGCGCGGCGGCCTGTTGGCAACGGTGTTGGCAGCCCTGCTGTGGG
ATCCGGCGGGCACCCCTGCCGAGACGGTGGAAGCCGAGCTGGAACGGCTGCGCTTTAGCCGCGCCGAGATGCGGGCCATC
CAGAAGCTGCACCAGCTTCTACCCCTGTTGCAAACGCTTCTGACCCATTCCCCCACTCCCGGACAACAGTGGCGGCTCTA
TCACCAGGCGGGATCCCTCTTCGGCGGCCTGGCCCTGCTGGCAGCAGCCACCGGCAGCAGCCCCCGAGAGATAGAATCCT
GGCTCCGGCGCTACGAAGATCCCCAGGATCCCCTGGCCCATTTGGTGCCCCTGCTGGATGGGAACGACCTGCTGCACAGC
TTGGGTGCTAAACCCGGACCCTGGGTGGGCCAGTTGCTGCAGGCTCTACAGGAGGCGCAGGCCGCGGGGCAGATCCAAAC
CCGCGCCGAAGCCCTCTCTTTTGCCCGCCGCTGGCGTGAGAGGAGTTCCCAACCCTAG

Upstream 100 bases:

>100_bases
TTTTGCGCAGCGGTCAGGTGAGGGTACGGCCTGTCTGGTTGCTTGCGCTCTGGGGATCCTAGCCTTTTAGATCATCGAAG
TCTCCCCCCTGTGCACATCG

Downstream 100 bases:

>100_bases
CCAAAGCGATAGCCGAAGCCGCGCACCGTCACCAAATACTCTGGGTTGCTGGGATCCAGCTCCAACTTTTCCCGCAGCCA
GCGGATGTGGACGTCCACCG

Product: polyA polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSEALLSKWLLTYFRQRLPFPKEWLPQGSYWVGGSLRDAYLAWLRGQAGDSKGPVDLDLMCPADPVGWAAGVARRLKAGF
VVLDAERQIARIVLGQGGASTPTAATVDAALQVGPTPEADLRQRDFTCNALALELQEGHLLDPTGGRADIEQRCLRMVHP
DNLSADPVRLLRAYRQAAQLGFALDPATAEAIRQRAPLLARVAAERVRAEVVTLLETGIPGLAHLQAAVAAGLLAAWLPR
LAPQSPGFAQAQAVWQWAEIWQLQYPRTLAELAQPLADRRGGLLATVLAALLWDPAGTPAETVEAELERLRFSRAEMRAI
QKLHQLLPLLQTLLTHSPTPGQQWRLYHQAGSLFGGLALLAAATGSSPREIESWLRRYEDPQDPLAHLVPLLDGNDLLHS
LGAKPGPWVGQLLQALQEAQAAGQIQTRAEALSFARRWRERSSQP

Sequences:

>Translated_445_residues
MSEALLSKWLLTYFRQRLPFPKEWLPQGSYWVGGSLRDAYLAWLRGQAGDSKGPVDLDLMCPADPVGWAAGVARRLKAGF
VVLDAERQIARIVLGQGGASTPTAATVDAALQVGPTPEADLRQRDFTCNALALELQEGHLLDPTGGRADIEQRCLRMVHP
DNLSADPVRLLRAYRQAAQLGFALDPATAEAIRQRAPLLARVAAERVRAEVVTLLETGIPGLAHLQAAVAAGLLAAWLPR
LAPQSPGFAQAQAVWQWAEIWQLQYPRTLAELAQPLADRRGGLLATVLAALLWDPAGTPAETVEAELERLRFSRAEMRAI
QKLHQLLPLLQTLLTHSPTPGQQWRLYHQAGSLFGGLALLAAATGSSPREIESWLRRYEDPQDPLAHLVPLLDGNDLLHS
LGAKPGPWVGQLLQALQEAQAAGQIQTRAEALSFARRWRERSSQP
>Mature_444_residues
SEALLSKWLLTYFRQRLPFPKEWLPQGSYWVGGSLRDAYLAWLRGQAGDSKGPVDLDLMCPADPVGWAAGVARRLKAGFV
VLDAERQIARIVLGQGGASTPTAATVDAALQVGPTPEADLRQRDFTCNALALELQEGHLLDPTGGRADIEQRCLRMVHPD
NLSADPVRLLRAYRQAAQLGFALDPATAEAIRQRAPLLARVAAERVRAEVVTLLETGIPGLAHLQAAVAAGLLAAWLPRL
APQSPGFAQAQAVWQWAEIWQLQYPRTLAELAQPLADRRGGLLATVLAALLWDPAGTPAETVEAELERLRFSRAEMRAIQ
KLHQLLPLLQTLLTHSPTPGQQWRLYHQAGSLFGGLALLAAATGSSPREIESWLRRYEDPQDPLAHLVPLLDGNDLLHSL
GAKPGPWVGQLLQALQEAQAAGQIQTRAEALSFARRWRERSSQP

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=202, Percent_Identity=30.1980198019802, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48586; Mature: 48455

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALLSKWLLTYFRQRLPFPKEWLPQGSYWVGGSLRDAYLAWLRGQAGDSKGPVDLDLM
CCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEEE
CPADPVGWAAGVARRLKAGFVVLDAERQIARIVLGQGGASTPTAATVDAALQVGPTPEAD
CCCCCCHHHHHHHHHHHCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHH
LRQRDFTCNALALELQEGHLLDPTGGRADIEQRCLRMVHPDNLSADPVRLLRAYRQAAQL
HHHCCCCHHHHEEEECCCCEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GFALDPATAEAIRQRAPLLARVAAERVRAEVVTLLETGIPGLAHLQAAVAAGLLAAWLPR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LAPQSPGFAQAQAVWQWAEIWQLQYPRTLAELAQPLADRRGGLLATVLAALLWDPAGTPA
HCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCH
ETVEAELERLRFSRAEMRAIQKLHQLLPLLQTLLTHSPTPGQQWRLYHQAGSLFGGLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AAATGSSPREIESWLRRYEDPQDPLAHLVPLLDGNDLLHSLGAKPGPWVGQLLQALQEAQ
HHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
AAGQIQTRAEALSFARRWRERSSQP
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEALLSKWLLTYFRQRLPFPKEWLPQGSYWVGGSLRDAYLAWLRGQAGDSKGPVDLDLM
CHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEEE
CPADPVGWAAGVARRLKAGFVVLDAERQIARIVLGQGGASTPTAATVDAALQVGPTPEAD
CCCCCCHHHHHHHHHHHCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHH
LRQRDFTCNALALELQEGHLLDPTGGRADIEQRCLRMVHPDNLSADPVRLLRAYRQAAQL
HHHCCCCHHHHEEEECCCCEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GFALDPATAEAIRQRAPLLARVAAERVRAEVVTLLETGIPGLAHLQAAVAAGLLAAWLPR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LAPQSPGFAQAQAVWQWAEIWQLQYPRTLAELAQPLADRRGGLLATVLAALLWDPAGTPA
HCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCH
ETVEAELERLRFSRAEMRAIQKLHQLLPLLQTLLTHSPTPGQQWRLYHQAGSLFGGLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AAATGSSPREIESWLRRYEDPQDPLAHLVPLLDGNDLLHSLGAKPGPWVGQLLQALQEAQ
HHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
AAGQIQTRAEALSFARRWRERSSQP
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]