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The map label for this gene is phoA [H]

Identifier: 86605488

GI number: 86605488

Start: 785884

End: 787233

Strand: Reverse

Name: phoA [H]

Synonym: CYA_0781

Alternate gene names: 86605488

Gene position: 787233-785884 (Counterclockwise)

Preceding gene: 86605489

Following gene: 86605484

Centisome position: 26.84

GC content: 58.89

Gene sequence:

>1350_bases
GTGTTAGCTACGCGTAGCTATTCTCTGAGGGCAGCGTTGGCTGCAACTTTGTGTCTGCTGTCCTCGTCAGTTTTTTCCTC
CAGTGTGGCTCAAGCGGCGCCTAAGCCAACCGGCAACGGTGCAATCTTCTTTCACCCAGATGGAACCTCAGCTTCCCACT
GGGACATCACACGCATTCTCTACTACGGCCCCGATGGCTTTCTGAACTGGGATCGTCTCCCCGTGATTGCCCCCTATCGC
GGGCACCTTCTCGACCAGCTTGGGGGAACTTCCAACGCCGGAGCGGTGACCCATGCCACGGGAACCCGTGTCCACGCCAA
GAGCTTTGGGTTGGATCCGGACGGCAAAGAGTACCCCTCTGCCAACGGCACCCTGAACACGATCATGGAAGAGGCCGTGG
CGGCAGGCTTGGGTACGGCTCTGGTGCAAACGGGATCCATCATTGAGCCGGGGACAGCTGCCTTCGTTGCCGAAGTTGCC
AAGCGCAGCGACTATGAGGAGATTGCCCTGCAGGTGGTGGAGTCAGGGGTAGACATCATCCTCGGCGCTGGCGAGGAATG
GCTGCTGCCGGAAGGTGTCCAGGGCCGCTTTGGCAAAGGGAAGCGCAAGGATGGCCGCAACCTGATTGAGGAGGCCAAGA
AAAAGGGGTACGCCGTTGTCTACACCCGCGACGAGCTGCTCAAGCTGCCGGCAGATGTCTCCAAAGTTTTGGGGGTGTTC
AACGTCGAGGACACCTTCTACGACAAGCCTGAAGAAGAGTTGCGGAAAGAAAACTTGCCCAACTACATCGCCAGCGCCCC
CACCATTGCCGAGATGACCGAATTTGCCCTGGCGCGGATCTCCCGCAATCCTAAGGGCTTCTTCGCCGTCATCGAAGAAG
AGGGCACTGATAACATGTGCAACAACCTCAATGCCTCTGGCTGCCTGGAGGCCATGAAGCGGGCCGACGATGCCATCGGG
GTGATTCTCAACTTTATCGAGAAGAATCCCAAGACCTTCATGATCACCACCAGCGACAGCAATGCCGGTGGCATGCAGTT
GGTGGATGTCGAGTCCGCCGATGAGCCGTTGCCCCCTACTGACCCCGAAGGCAGTGGTGCCCCCATTGACGGAGTTGACG
GCACGGGCACAAAGCCGTTTATTTCTGCTCCTGACAAGAATGGCAAGCGCTTTCCCTTTGCCGTGGCTTGGGCTACCGGA
GATGACGTGGGCAGCGGCGTCATCGCTCGCGCTGCGGGCTTGAAGGCCCAAGAGCTGGTGCCGACCACCGGGATCCTGAA
CACGGACATTTATCGGATTCTCTACTTCGTGCTCTTCGGGGAGCAGATCCCCAGCGAGCTCAAGCCGTAG

Upstream 100 bases:

>100_bases
CGCTTCAGGCACGTTTCGCGCTGCTGCATTGCTATGCCCTTGCAAACTCATCTGAAGCTGTGTTACGTTTTTCAACGTTC
AGCTTCGAAGGAGCAAGGGC

Downstream 100 bases:

>100_bases
TCTCAGGCAGCCCGGCCTTGGCTTGGAACTTCTGGGGACGGAGGGGATCCCGGCGATCCCCTTTTTATTTACGCAACGCT
GTCGCGAACGCGCCGCTCCA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MLATRSYSLRAALAATLCLLSSSVFSSSVAQAAPKPTGNGAIFFHPDGTSASHWDITRILYYGPDGFLNWDRLPVIAPYR
GHLLDQLGGTSNAGAVTHATGTRVHAKSFGLDPDGKEYPSANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAEVA
KRSDYEEIALQVVESGVDIILGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMCNNLNASGCLEAMKRADDAIG
VILNFIEKNPKTFMITTSDSNAGGMQLVDVESADEPLPPTDPEGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATG
DDVGSGVIARAAGLKAQELVPTTGILNTDIYRILYFVLFGEQIPSELKP

Sequences:

>Translated_449_residues
MLATRSYSLRAALAATLCLLSSSVFSSSVAQAAPKPTGNGAIFFHPDGTSASHWDITRILYYGPDGFLNWDRLPVIAPYR
GHLLDQLGGTSNAGAVTHATGTRVHAKSFGLDPDGKEYPSANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAEVA
KRSDYEEIALQVVESGVDIILGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMCNNLNASGCLEAMKRADDAIG
VILNFIEKNPKTFMITTSDSNAGGMQLVDVESADEPLPPTDPEGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATG
DDVGSGVIARAAGLKAQELVPTTGILNTDIYRILYFVLFGEQIPSELKP
>Mature_449_residues
MLATRSYSLRAALAATLCLLSSSVFSSSVAQAAPKPTGNGAIFFHPDGTSASHWDITRILYYGPDGFLNWDRLPVIAPYR
GHLLDQLGGTSNAGAVTHATGTRVHAKSFGLDPDGKEYPSANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAEVA
KRSDYEEIALQVVESGVDIILGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMCNNLNASGCLEAMKRADDAIG
VILNFIEKNPKTFMITTSDSNAGGMQLVDVESADEPLPPTDPEGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATG
DDVGSGVIARAAGLKAQELVPTTGILNTDIYRILYFVLFGEQIPSELKP

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=317, Percent_Identity=28.7066246056782, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI94721246, Length=313, Percent_Identity=27.7955271565495, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157266292, Length=313, Percent_Identity=27.7955271565495, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI116734717, Length=317, Percent_Identity=25.2365930599369, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI294660770, Length=316, Percent_Identity=25, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI48994877, Length=355, Percent_Identity=27.3239436619718, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320689, Length=355, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585213, Length=354, Percent_Identity=25.7062146892655, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21358067, Length=315, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85725200, Length=330, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85815833, Length=330, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355151, Length=384, Percent_Identity=23.6979166666667, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47879; Mature: 47879

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATRSYSLRAALAATLCLLSSSVFSSSVAQAAPKPTGNGAIFFHPDGTSASHWDITRIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEE
YYGPDGFLNWDRLPVIAPYRGHLLDQLGGTSNAGAVTHATGTRVHAKSFGLDPDGKEYPS
EECCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCEEEECCCCEEEHHHCCCCCCCCCCCC
ANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAEVAKRSDYEEIALQVVESGVDII
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
LGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHEE
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMC
CCCHHHCCCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
NNLNASGCLEAMKRADDAIGVILNFIEKNPKTFMITTSDSNAGGMQLVDVESADEPLPPT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCC
DPEGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATGDDVGSGVIARAAGLKAQELV
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCHHHHC
PTTGILNTDIYRILYFVLFGEQIPSELKP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLATRSYSLRAALAATLCLLSSSVFSSSVAQAAPKPTGNGAIFFHPDGTSASHWDITRIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEE
YYGPDGFLNWDRLPVIAPYRGHLLDQLGGTSNAGAVTHATGTRVHAKSFGLDPDGKEYPS
EECCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCEEEECCCCEEEHHHCCCCCCCCCCCC
ANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAEVAKRSDYEEIALQVVESGVDII
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
LGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHEE
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMC
CCCHHHCCCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
NNLNASGCLEAMKRADDAIGVILNFIEKNPKTFMITTSDSNAGGMQLVDVESADEPLPPT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCC
DPEGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATGDDVGSGVIARAAGLKAQELV
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCHHHHC
PTTGILNTDIYRILYFVLFGEQIPSELKP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]