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The map label for this gene is tenA [H]

Identifier: 86605132

GI number: 86605132

Start: 434337

End: 434996

Strand: Reverse

Name: tenA [H]

Synonym: CYA_0413

Alternate gene names: 86605132

Gene position: 434996-434337 (Counterclockwise)

Preceding gene: 86605133

Following gene: 86605127

Centisome position: 14.83

GC content: 59.55

Gene sequence:

>660_bases
ATGTCTCAATCTTTTACCGCCCACCTCTGGCAGACGATCGAGCCCATCTACCAACAGATCTTGGATCACCCCTTTAACCA
AGAGCTGGCCGCAGGCAGCCTCTCGCGGCAGCGCTTTCAGTTCTATCTGCAGCAGGATGCTCTTTATCTCACCGATTTTG
CCCGCGCCCTTGCCTTGATCGGGGCCCGTTCCGAGGGGGCTGAGCGGGTGGTGAGTTTCTTGAACTTTGCCCTGGGGGCC
ATTGTGGCCGAGCGCAGCCTGCACGAGAGTTACTTTCGCCTCTACGACATCCAGCCGGAGACAACCTACGCTCCTGCCTG
CTTCGCCTACACCCGTTTTCTATTGGCTGCGGCTGCCTTGGATCCCTACGAGGTGGCCATGGCCGCTGTGCTGCCCTGCT
TCTGGATTTACCGTGAGGTGGGATCCGCCATTTACCGCACCGCCAAGCCCAACAATCCTTATCAACAATGGATCGACACC
TATGCCGGGGAAGAATTTGCCCAGGTGGTGCAACAGGCCCTGGACATCACCGACTCCTTGGCTACGCAGACCACAGCGGC
GACGCGGGAGAAGATGACGGCTGCTTTTGTCACCGCCTCTCGCCTGGAATGGCTGTTCTGGGACAGCGCCTACCGCCTGG
AAACCTGGCAGCCCGCCTGA

Upstream 100 bases:

>100_bases
CGTCCAACTTTCCGGCCACAGGGCACCCAAAGAGTTTCCCCCACACCCAGAGATCTCAATCAGTTGAAAAGAACCCCAGA
AACGCCGCGAGAGAGCCACC

Downstream 100 bases:

>100_bases
TCCCCGCCGGGCCTGGCGGCTCAGCAAGGTCGTCATGGCCATGGCATGTGGTTATGGTCCAACGCCAAGATGACCCGCAC
CTGGGTCAACAGCTCCAGCT

Product: transcriptional activator TenA

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSQSFTAHLWQTIEPIYQQILDHPFNQELAAGSLSRQRFQFYLQQDALYLTDFARALALIGARSEGAERVVSFLNFALGA
IVAERSLHESYFRLYDIQPETTYAPACFAYTRFLLAAAALDPYEVAMAAVLPCFWIYREVGSAIYRTAKPNNPYQQWIDT
YAGEEFAQVVQQALDITDSLATQTTAATREKMTAAFVTASRLEWLFWDSAYRLETWQPA

Sequences:

>Translated_219_residues
MSQSFTAHLWQTIEPIYQQILDHPFNQELAAGSLSRQRFQFYLQQDALYLTDFARALALIGARSEGAERVVSFLNFALGA
IVAERSLHESYFRLYDIQPETTYAPACFAYTRFLLAAAALDPYEVAMAAVLPCFWIYREVGSAIYRTAKPNNPYQQWIDT
YAGEEFAQVVQQALDITDSLATQTTAATREKMTAAFVTASRLEWLFWDSAYRLETWQPA
>Mature_218_residues
SQSFTAHLWQTIEPIYQQILDHPFNQELAAGSLSRQRFQFYLQQDALYLTDFARALALIGARSEGAERVVSFLNFALGAI
VAERSLHESYFRLYDIQPETTYAPACFAYTRFLLAAAALDPYEVAMAAVLPCFWIYREVGSAIYRTAKPNNPYQQWIDTY
AGEEFAQVVQQALDITDSLATQTTAATREKMTAAFVTASRLEWLFWDSAYRLETWQPA

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=210, Percent_Identity=22.3809523809524, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6325378, Length=211, Percent_Identity=26.0663507109005, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324997, Length=209, Percent_Identity=22.9665071770335, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 24973; Mature: 24842

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSFTAHLWQTIEPIYQQILDHPFNQELAAGSLSRQRFQFYLQQDALYLTDFARALALI
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GARSEGAERVVSFLNFALGAIVAERSLHESYFRLYDIQPETTYAPACFAYTRFLLAAAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
DPYEVAMAAVLPCFWIYREVGSAIYRTAKPNNPYQQWIDTYAGEEFAQVVQQALDITDSL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ATQTTAATREKMTAAFVTASRLEWLFWDSAYRLETWQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQSFTAHLWQTIEPIYQQILDHPFNQELAAGSLSRQRFQFYLQQDALYLTDFARALALI
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GARSEGAERVVSFLNFALGAIVAERSLHESYFRLYDIQPETTYAPACFAYTRFLLAAAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
DPYEVAMAAVLPCFWIYREVGSAIYRTAKPNNPYQQWIDTYAGEEFAQVVQQALDITDSL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ATQTTAATREKMTAAFVTASRLEWLFWDSAYRLETWQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]