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The map label for this gene is moaA

Identifier: 86604900

GI number: 86604900

Start: 168940

End: 169878

Strand: Reverse

Name: moaA

Synonym: CYA_0172

Alternate gene names: 86604900

Gene position: 169878-168940 (Counterclockwise)

Preceding gene: 86604901

Following gene: 86604899

Centisome position: 5.79

GC content: 51.12

Gene sequence:

>939_bases
ATGCAGCTAGTGGATCAATGGAATCGTCGCATTCGCAAGCTAAGGGTTTCTCTAACCGATCAATGCAACCTTCGCTGCCG
CTACTGCATGCCTTTGCATCCAGAATTTCTGGACAAAAGTAGCTACTTAACTCCGCAGCAATACAAAGAAATTATTGGCG
AGTTGCTGGACTATGGCATTGAAGAAGTTCGCATTACAGGTGGTGAGCCTCTGGTTCGGCAAGACTTTGATGAAGTCATT
CAGGAACTTGCCAAGCTGAAGATCCCGTCCCTCAGCCTAACAACCAATGGCCTGCTGCTCCACCGCTACTGGGACGTTCT
CAAGGCTGCCAACGTTCTCAACCTTAATGTTAGCCTCGATAGCCTACAGCCCTCCACCCAAGCTGCAATTGCCCGTCGCG
ACTGCCTGGCGGATATTCTCCGTAATATCCAGGAGGGTATTGCCCGGGGATTTAGCCTCAAAGTCAATACGGTTGTAATG
CGGGGGATCAACGATTGCGAGCTATTTGATTTCGTTGAGTTTGCCCGCAAGACAGGCGTGACAGTTCGCTTTCTGGAGCT
GATGCGGGTCGGCTATGCCAACTCTCTCTATGAAGAACACTACGTTTCAGCACAAGAGTGCATTGCCCGTCTGCAAGAAC
GCTATACTCTAACCCCCCTTCCAGCGGCACCTGATTCGACAGCGTTTTACTTTACAATCGACGAGAATGTTAAAGTTGGA
TTTATTGCCTCTGAGTCTCAGCCCTTCTGCAGCAGCTGTTCCCGGTGGCGCTTGACAGCTGATGGTCGTCTCTTTGCCTG
CCTATTTAGTGAGGAGGGTCTCTCGGTTCGAGACAAAACACCAGGCCAACGTCAGCAAATTTACCAGCAGCTTTTGGGGA
TGAAACCACTGCGCCGGGTTGAGCAGGTCTCACACCCAATGTACGGCTTAGGGGGCTAG

Upstream 100 bases:

>100_bases
TTCATCGCTCTAACAGACAGCGATGGCTTTGTAGAGTTACCCCCGGCTCAAACTATCCTCAGCCCCGAGCAAAGCATCCA
ATTCTATCCGTGGTACTAAG

Downstream 100 bases:

>100_bases
CTCTATGGACAAAGCTGATCGTACATGGGCCCATCTTAATGCTCAAGGCCATCCGCATATGGTAGATATTAGCCACAAAT
CGGTGTCTTTGCGAACGGCC

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MQLVDQWNRRIRKLRVSLTDQCNLRCRYCMPLHPEFLDKSSYLTPQQYKEIIGELLDYGIEEVRITGGEPLVRQDFDEVI
QELAKLKIPSLSLTTNGLLLHRYWDVLKAANVLNLNVSLDSLQPSTQAAIARRDCLADILRNIQEGIARGFSLKVNTVVM
RGINDCELFDFVEFARKTGVTVRFLELMRVGYANSLYEEHYVSAQECIARLQERYTLTPLPAAPDSTAFYFTIDENVKVG
FIASESQPFCSSCSRWRLTADGRLFACLFSEEGLSVRDKTPGQRQQIYQQLLGMKPLRRVEQVSHPMYGLGG

Sequences:

>Translated_312_residues
MQLVDQWNRRIRKLRVSLTDQCNLRCRYCMPLHPEFLDKSSYLTPQQYKEIIGELLDYGIEEVRITGGEPLVRQDFDEVI
QELAKLKIPSLSLTTNGLLLHRYWDVLKAANVLNLNVSLDSLQPSTQAAIARRDCLADILRNIQEGIARGFSLKVNTVVM
RGINDCELFDFVEFARKTGVTVRFLELMRVGYANSLYEEHYVSAQECIARLQERYTLTPLPAAPDSTAFYFTIDENVKVG
FIASESQPFCSSCSRWRLTADGRLFACLFSEEGLSVRDKTPGQRQQIYQQLLGMKPLRRVEQVSHPMYGLGG
>Mature_312_residues
MQLVDQWNRRIRKLRVSLTDQCNLRCRYCMPLHPEFLDKSSYLTPQQYKEIIGELLDYGIEEVRITGGEPLVRQDFDEVI
QELAKLKIPSLSLTTNGLLLHRYWDVLKAANVLNLNVSLDSLQPSTQAAIARRDCLADILRNIQEGIARGFSLKVNTVVM
RGINDCELFDFVEFARKTGVTVRFLELMRVGYANSLYEEHYVSAQECIARLQERYTLTPLPAAPDSTAFYFTIDENVKVG
FIASESQPFCSSCSRWRLTADGRLFACLFSEEGLSVRDKTPGQRQQIYQQLLGMKPLRRVEQVSHPMYGLGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=306, Percent_Identity=34.9673202614379, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI22027536, Length=306, Percent_Identity=35.2941176470588, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786999, Length=290, Percent_Identity=33.448275862069, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32566331, Length=307, Percent_Identity=31.9218241042345, Blast_Score=167, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32566329, Length=326, Percent_Identity=31.2883435582822, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17561210, Length=286, Percent_Identity=33.2167832167832, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28574488, Length=328, Percent_Identity=34.4512195121951, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI28574490, Length=280, Percent_Identity=36.7857142857143, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35672; Mature: 35672

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVDQWNRRIRKLRVSLTDQCNLRCRYCMPLHPEFLDKSSYLTPQQYKEIIGELLDYGI
CCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCC
EEVRITGGEPLVRQDFDEVIQELAKLKIPSLSLTTNGLLLHRYWDVLKAANVLNLNVSLD
CEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCEEEEEEEHH
SLQPSTQAAIARRDCLADILRNIQEGIARGFSLKVNTVVMRGINDCELFDFVEFARKTGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
TVRFLELMRVGYANSLYEEHYVSAQECIARLQERYTLTPLPAAPDSTAFYFTIDENVKVG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCEEEE
FIASESQPFCSSCSRWRLTADGRLFACLFSEEGLSVRDKTPGQRQQIYQQLLGMKPLRRV
EEECCCCCHHHCCCCEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
EQVSHPMYGLGG
HHHCCCCCCCCC
>Mature Secondary Structure
MQLVDQWNRRIRKLRVSLTDQCNLRCRYCMPLHPEFLDKSSYLTPQQYKEIIGELLDYGI
CCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCC
EEVRITGGEPLVRQDFDEVIQELAKLKIPSLSLTTNGLLLHRYWDVLKAANVLNLNVSLD
CEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCEEEEEEEHH
SLQPSTQAAIARRDCLADILRNIQEGIARGFSLKVNTVVMRGINDCELFDFVEFARKTGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
TVRFLELMRVGYANSLYEEHYVSAQECIARLQERYTLTPLPAAPDSTAFYFTIDENVKVG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCEEEE
FIASESQPFCSSCSRWRLTADGRLFACLFSEEGLSVRDKTPGQRQQIYQQLLGMKPLRRV
EEECCCCCHHHCCCCEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
EQVSHPMYGLGG
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]