Click here to switch to the map view.

The map label for this gene is rutB [H]

Identifier: 86361273

GI number: 86361273

Start: 608847

End: 609482

Strand: Direct

Name: rutB [H]

Synonym: RHE_PF00544

Alternate gene names: 86361273

Gene position: 608847-609482 (Clockwise)

Preceding gene: 86361272

Following gene: 86361274

Centisome position: 94.76

GC content: 60.53

Gene sequence:

>636_bases
ATGACAGAATTGTCCTACGATTGCAGCAACACGGGATTGATCGTCGTCGATCCCTATAACGACTTCATCTCGGAAGGCGG
GAAGATCTGGCCCCGGCTCAAGGAGGTTGCCGAGGCGAACAATTGCGTTCCGCACATGCTCGAAGTGTTGAACGCCGCGC
GGCAGGCAAGGCTGCGCGTCTTCTTTGCGATGCACCACCGCTACAGGCCCGGCGATTACGAAAACTGGAAATTCGTTGCG
CCAATCCAGCGCGCGGCATGGCATCGCAGGAGCTTCGAGTTCGGCACCTGGGGCGGCGAATTCCGCGCAGAATTCGTGCC
GCTGGACGGCGAGGTCGTCGCCTCGGAGCACTGGTGCTCGAGCGGCTTCGCCAACACGGATCTCGATCTCGAGTTGAAGC
GGCACGGGATCCAGAGAGTGATCGTGATCGGCCTCGTCGCGCACACCTGCATCGAGGCCACGGTTCGCTACGCTGCCGAG
CTCGGCTATGATGTGACGGTAGTGAAGGACGCCGTTGCAGACTACTCTCCGGAGATGATGCACGCGGCGCTCGAGATCAA
TTTGCCGAACTATGCCAGCGCGATCGTGGAGACAGGGGAGGTCGTTACCGCTCTCTCCGCAAGCAACGCAGCATAG

Upstream 100 bases:

>100_bases
ACAACAAGGGCGAAACGCCGCTTTTCGCCGCAAGCATTGAACGCGCTGCCCTGCGCAACGCCTCTTCGATCAAGGCAGCG
TGATTTCCGGGAGGATTACC

Downstream 100 bases:

>100_bases
TCACACCAACCCGCCACGGCGCAGTGATGCTCCGGCTGACCAACACCTATGCATAATGGACAGCCGTCTGGTCGGGGCGC
ATCGTCTCTCCGTTGCGGCA

Product: isochorismatase protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTELSYDCSNTGLIVVDPYNDFISEGGKIWPRLKEVAEANNCVPHMLEVLNAARQARLRVFFAMHHRYRPGDYENWKFVA
PIQRAAWHRRSFEFGTWGGEFRAEFVPLDGEVVASEHWCSSGFANTDLDLELKRHGIQRVIVIGLVAHTCIEATVRYAAE
LGYDVTVVKDAVADYSPEMMHAALEINLPNYASAIVETGEVVTALSASNAA

Sequences:

>Translated_211_residues
MTELSYDCSNTGLIVVDPYNDFISEGGKIWPRLKEVAEANNCVPHMLEVLNAARQARLRVFFAMHHRYRPGDYENWKFVA
PIQRAAWHRRSFEFGTWGGEFRAEFVPLDGEVVASEHWCSSGFANTDLDLELKRHGIQRVIVIGLVAHTCIEATVRYAAE
LGYDVTVVKDAVADYSPEMMHAALEINLPNYASAIVETGEVVTALSASNAA
>Mature_210_residues
TELSYDCSNTGLIVVDPYNDFISEGGKIWPRLKEVAEANNCVPHMLEVLNAARQARLRVFFAMHHRYRPGDYENWKFVAP
IQRAAWHRRSFEFGTWGGEFRAEFVPLDGEVVASEHWCSSGFANTDLDLELKRHGIQRVIVIGLVAHTCIEATVRYAAEL
GYDVTVVKDAVADYSPEMMHAALEINLPNYASAIVETGEVVTALSASNAA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=218, Percent_Identity=22.9357798165138, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 23562; Mature: 23431

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELSYDCSNTGLIVVDPYNDFISEGGKIWPRLKEVAEANNCVPHMLEVLNAARQARLRV
CCCCCCCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FFAMHHRYRPGDYENWKFVAPIQRAAWHRRSFEFGTWGGEFRAEFVPLDGEVVASEHWCS
HHHHHHCCCCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEECCHHHC
SGFANTDLDLELKRHGIQRVIVIGLVAHTCIEATVRYAAELGYDVTVVKDAVADYSPEMM
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHE
HAALEINLPNYASAIVETGEVVTALSASNAA
EEEEEECCCHHHHHHHHCCHHHEEECCCCCC
>Mature Secondary Structure 
TELSYDCSNTGLIVVDPYNDFISEGGKIWPRLKEVAEANNCVPHMLEVLNAARQARLRV
CCCCCCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FFAMHHRYRPGDYENWKFVAPIQRAAWHRRSFEFGTWGGEFRAEFVPLDGEVVASEHWCS
HHHHHHCCCCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEECCHHHC
SGFANTDLDLELKRHGIQRVIVIGLVAHTCIEATVRYAAELGYDVTVVKDAVADYSPEMM
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHE
HAALEINLPNYASAIVETGEVVTALSASNAA
EEEEEECCCHHHHHHHHCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA