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The map label for this gene is lutR [H]

Identifier: 86361110

GI number: 86361110

Start: 428555

End: 429247

Strand: Reverse

Name: lutR [H]

Synonym: RHE_PF00380

Alternate gene names: 86361110

Gene position: 429247-428555 (Counterclockwise)

Preceding gene: 86361111

Following gene: 86361109

Centisome position: 66.81

GC content: 61.62

Gene sequence:

>693_bases
ATGAAAGAAAACTCCCTCCTCTCCGATCTTGCGGCACATCTTTTCTCAAACTTGAGCGGCAACGGCCGCACGCCATCGGA
GCGCGAACTTGCGGAGCACTTCAGCGTCAGCCGCGGTCAGATTCGCGAGGCGCTTGCCATTCTGGAGGCGATGCGCATCG
TCGAGCGCCGAGCCAAGTCGGGCATCTACCTGACGACGACGGAAGCGAGCGTGGAGGCAATGGCGCTTTTTGCCCGCGCC
GGCGTTCCGCTTGATCCCATCCTGATCTACGAGACCGTGGAGCTTCGCAAGATCCATGAGATCAAGGCGGCAGAACTCGC
CTGCGATCGGGCGACGGAGGAGAATTACAAGCGCTTGCGCGATATCCTCGCAGCATCCGAGGCGAAGCTTGCCGCGGGCG
AGGGGCTGGCGCGCGAGGACCGGGATTTTCATTTGGAGATCGTCCGCGCCACCAAGAATAGTGTCTTTTATCGGGTGTGC
AGCGTCTACTACGTGATGGGCGAGCACCGGCTGCCGATCTATTTTGCCGATGCTGCCCGCAGCCGGCGCTCGCATGAGGA
GCATATCCGCATCTACGAGGCGCTGCTTGCCCGAGACGGCAATCTCGCCCAGGCGCTGATGAGCGCGCATCTTCAAGGTG
CGCAAAGCTATTGGAAGGGTCTCATCGGCGGTCCGGCGGCGGCAGCTCGATAG

Upstream 100 bases:

>100_bases
TGTCTGTAAAGGACGGTATGATAGAGATCCCGGACAGACCTGGCCTGGGCTTCACGATCAATCAGCGGGTCCTGGAGACT
CATGCGCAAAGACACTGACA

Downstream 100 bases:

>100_bases
GGAGTAACCGGCGGGGAGGGCCGATGAGCTTCATCTTTTCCACACATCCCCTGCACCCCGCGGCCAAGGCCATGCTTCAG
GGAGCGGGTGATCTGCGTAT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKENSLLSDLAAHLFSNLSGNGRTPSERELAEHFSVSRGQIREALAILEAMRIVERRAKSGIYLTTTEASVEAMALFARA
GVPLDPILIYETVELRKIHEIKAAELACDRATEENYKRLRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFYRVC
SVYYVMGEHRLPIYFADAARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAQSYWKGLIGGPAAAAR

Sequences:

>Translated_230_residues
MKENSLLSDLAAHLFSNLSGNGRTPSERELAEHFSVSRGQIREALAILEAMRIVERRAKSGIYLTTTEASVEAMALFARA
GVPLDPILIYETVELRKIHEIKAAELACDRATEENYKRLRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFYRVC
SVYYVMGEHRLPIYFADAARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAQSYWKGLIGGPAAAAR
>Mature_230_residues
MKENSLLSDLAAHLFSNLSGNGRTPSERELAEHFSVSRGQIREALAILEAMRIVERRAKSGIYLTTTEASVEAMALFARA
GVPLDPILIYETVELRKIHEIKAAELACDRATEENYKRLRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFYRVC
SVYYVMGEHRLPIYFADAARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAQSYWKGLIGGPAAAAR

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=201, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082208, Length=205, Percent_Identity=26.8292682926829, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25626; Mature: 25626

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENSLLSDLAAHLFSNLSGNGRTPSERELAEHFSVSRGQIREALAILEAMRIVERRAKS
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
GIYLTTTEASVEAMALFARAGVPLDPILIYETVELRKIHEIKAAELACDRATEENYKRLR
CEEEEECCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFYRVCSVYYVMGEHRLPIYFADAAR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHH
SRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAQSYWKGLIGGPAAAAR
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MKENSLLSDLAAHLFSNLSGNGRTPSERELAEHFSVSRGQIREALAILEAMRIVERRAKS
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
GIYLTTTEASVEAMALFARAGVPLDPILIYETVELRKIHEIKAAELACDRATEENYKRLR
CEEEEECCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFYRVCSVYYVMGEHRLPIYFADAAR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHH
SRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAQSYWKGLIGGPAAAAR
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]