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The map label for this gene is dgoD [H]

Identifier: 86361111

GI number: 86361111

Start: 429250

End: 430419

Strand: Reverse

Name: dgoD [H]

Synonym: RHE_PF00381

Alternate gene names: 86361111

Gene position: 430419-429250 (Counterclockwise)

Preceding gene: 86361112

Following gene: 86361110

Centisome position: 66.99

GC content: 62.65

Gene sequence:

>1170_bases
GTGCGTATCAAGACCGTCGAGGCCTGGTGGGTCAAGATTCCCATCGAGGCGAGCCTTCAGCACCGCAGCGACTTCGGCCG
CCTCACGACTTTTGATGCTGCGATTCTGCGTATCGAAACGAACGACGGCATCGTGGGTTGGGGAGAGGGTAAGAATGCCG
CAGGCAGTGCCGGCACCTACGGCACGCTGGTACACATGATCAATCACGAGGTAGGGCCAAAGCTCATCGGCCGCGATCCT
GGCGACATCTCCACCATATGGGAGATGCTCTATAACGGCGTACGCCACGAGACGGCGGCGAGTTCGGGCCATGCCATGCC
CGAGATCGCGCGGCGCGGCCTTTCCGTTGCGGCGATCAGCGCGGTCGACATCGCACTCTGGGACATTCTCGGCAAGTCGC
TCGGCGTCCCGGTCTGGAAGCTGCTGGGTGGCCGCAAGGCCGACAGGTTGCCAGCCTATGCCTCGGGCGGTTGGGAGAGT
GCGGAGAAGATCGGCGAGCAACTCCGGTCGTATATCGCGGTCGGCGGTTTCAAGTCAGTCAAGATGCGGGTCGGCGCGAT
GGATCGTGCGCCGCATGTTTCGGCGGCGCGCGTGCGGGCGGCTCGCAAAGCTGTCGGCCCCGACGTCGACCTGATGGTCG
ATGCGCACGGCACCTATACTGTTGCCGAAGCGAAGCGGTTCATCCAGATGGTTGCCGATTGCGACCTCGCCTGGTTCGAG
GAGCCGGTGATAGCCGATGACAAGCCTGGCTTGGCGGAGGTGCGCGCTGCCGGGAATGTGCCGATCGCTGCCGGCGAGAG
CGAGGCGACGCGTTTTGCCTTCCGAGATCTTGCCATGCTTCGGGCCGCCGACATATTCCAGCCCGATCCGGCCTTCTGTG
GCGGCATTACGGAGGCGATGCGCATCAGTTCGCTCGCTAGCGCCTTCAACCTCCGTTTCGCACCGCATCTTTGGGCCGGT
GCGCCCTGCTTCTTCTCCGGCCTGCACTTGTGCGCGGCCTCCCCGGCAAGCTTCGTCATCGAATACTCACTCGGCGCAAA
TCCGATGATCCATGATCTTGTCGAGGACCCTGTGTCTGTAAAGGACGGTATGATAGAGATCCCGGACAGACCTGGCCTGG
GCTTCACGATCAATCAGCGGGTCCTGGAGACTCATGCGCAAAGACACTGA

Upstream 100 bases:

>100_bases
GGATTACCGCCGAGACGATCCTGCCGCTAATTAAGTAACGAACTGAAGACATGTGCCCGCGGGCAGCGGCCCGCGGGCCG
TGAGGAGGGGGATCCGGGAC

Downstream 100 bases:

>100_bases
CAATGAAAGAAAACTCCCTCCTCTCCGATCTTGCGGCACATCTTTTCTCAAACTTGAGCGGCAACGGCCGCACGCCATCG
GAGCGCGAACTTGCGGAGCA

Product: putative mandelate racemase protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRIKTVEAWWVKIPIEASLQHRSDFGRLTTFDAAILRIETNDGIVGWGEGKNAAGSAGTYGTLVHMINHEVGPKLIGRDP
GDISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWES
AEKIGEQLRSYIAVGGFKSVKMRVGAMDRAPHVSAARVRAARKAVGPDVDLMVDAHGTYTVAEAKRFIQMVADCDLAWFE
EPVIADDKPGLAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEAMRISSLASAFNLRFAPHLWAG
APCFFSGLHLCAASPASFVIEYSLGANPMIHDLVEDPVSVKDGMIEIPDRPGLGFTINQRVLETHAQRH

Sequences:

>Translated_389_residues
MRIKTVEAWWVKIPIEASLQHRSDFGRLTTFDAAILRIETNDGIVGWGEGKNAAGSAGTYGTLVHMINHEVGPKLIGRDP
GDISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWES
AEKIGEQLRSYIAVGGFKSVKMRVGAMDRAPHVSAARVRAARKAVGPDVDLMVDAHGTYTVAEAKRFIQMVADCDLAWFE
EPVIADDKPGLAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEAMRISSLASAFNLRFAPHLWAG
APCFFSGLHLCAASPASFVIEYSLGANPMIHDLVEDPVSVKDGMIEIPDRPGLGFTINQRVLETHAQRH
>Mature_389_residues
MRIKTVEAWWVKIPIEASLQHRSDFGRLTTFDAAILRIETNDGIVGWGEGKNAAGSAGTYGTLVHMINHEVGPKLIGRDP
GDISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWES
AEKIGEQLRSYIAVGGFKSVKMRVGAMDRAPHVSAARVRAARKAVGPDVDLMVDAHGTYTVAEAKRFIQMVADCDLAWFE
EPVIADDKPGLAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEAMRISSLASAFNLRFAPHLWAG
APCFFSGLHLCAASPASFVIEYSLGANPMIHDLVEDPVSVKDGMIEIPDRPGLGFTINQRVLETHAQRH

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=413, Percent_Identity=24.455205811138, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI186972148, Length=331, Percent_Identity=24.1691842900302, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI48994953, Length=362, Percent_Identity=30.6629834254144, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787864, Length=383, Percent_Identity=26.1096605744125, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI226510960, Length=370, Percent_Identity=26.2162162162162, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 41918; Mature: 41918

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKTVEAWWVKIPIEASLQHRSDFGRLTTFDAAILRIETNDGIVGWGEGKNAAGSAGTY
CCEEEEEEEEEEEEHHHCHHHHCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHH
GTLVHMINHEVGPKLIGRDPGDISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAIS
HHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHCCCCEEHHH
AVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWESAEKIGEQLRSYIAVGGFKSV
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
KMRVGAMDRAPHVSAARVRAARKAVGPDVDLMVDAHGTYTVAEAKRFIQMVADCDLAWFE
HEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHCCCHHHCC
EPVIADDKPGLAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEAM
CCCCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFVIEYSLGANPMIHDLVEDPVSV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCC
KDGMIEIPDRPGLGFTINQRVLETHAQRH
CCCEEECCCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure
MRIKTVEAWWVKIPIEASLQHRSDFGRLTTFDAAILRIETNDGIVGWGEGKNAAGSAGTY
CCEEEEEEEEEEEEHHHCHHHHCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHH
GTLVHMINHEVGPKLIGRDPGDISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAIS
HHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHCCCCEEHHH
AVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWESAEKIGEQLRSYIAVGGFKSV
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
KMRVGAMDRAPHVSAARVRAARKAVGPDVDLMVDAHGTYTVAEAKRFIQMVADCDLAWFE
HEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHCCCHHHCC
EPVIADDKPGLAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEAM
CCCCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFVIEYSLGANPMIHDLVEDPVSV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCC
KDGMIEIPDRPGLGFTINQRVLETHAQRH
CCCEEECCCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]