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The map label for this gene is dapAf1

Identifier: 86360827

GI number: 86360827

Start: 95643

End: 96533

Strand: Direct

Name: dapAf1

Synonym: RHE_PF00094

Alternate gene names: 86360827

Gene position: 95643-96533 (Clockwise)

Preceding gene: 86360826

Following gene: 86360828

Centisome position: 14.89

GC content: 61.95

Gene sequence:

>891_bases
ATGAAGTTGACATCAAGATTCGGGCTTTCCGTCGCTCTCGCCACGCCGTTCGACAGCGATGGCAATATTGCGATCGAGGC
GATGATCGCGCAGGCGCGACGCAGCCTTGCGGCCGGATGCTCCAGCGTGACGCTGTTCGGGACGACGGGCGAGGGCTCCT
CCGTCGGGAACGGCGAACGGGAACGCGTGCTGGCCGCCTTTCTCGATGCCGGTATTGCGCCTGGCAACATTATCGTCGGC
GTGCTGGTCGATGCCGCAGAGGATGCGGCGATGCAGGCCGCTCAGGCTCTGTCCAGGGGCGCCCGCAACATCCTTCTTGC
CCCGCCGTCCTATTTCAAGAACGTCAGTGACGACGGCGTCTTTCAATGGTTTTCCAACGTGTTCGCTCTTCTGGGCGACA
CGGCGCGGGATATCATCGTCTACAATCTTCCCTCGCTCACCATGGTGCCGCTGAGCGTGTCGCTGATAGGCCGGCTGCGG
ACCGCCTTTCCCCATATCGTCACGGGCGTCAAGGATTCCTCCGGCGACTGGCCGTACACGGAAGCGCTTTTGGAAGCCCA
TAGTGATCTCATCATCCTGGTCGGCGACGAGCGGCACCTCGCAAGGGCCGTGCGGCTCGGCGGCCAGGGTGCTATATCGG
GCATGGCGAATTTCGTGCCCCACGAACTCAAGCCGATGGCCGAAGAGGGTAAGGACGATAGCCGCGTCGCCGATTTCGTC
CTTGAATTGCTGAAACATCCTGTCGTTCCGGCCGTGAAGGCGATGGTCGCGCGCACGACGTCGGAGGAGACATGGATCGC
GGTCCGTCCGCCGCTTACTTCGATATCCGGTGAAGCGCGGGCGCAACTCGCTCTGGCATTCGATAATCTTTTCCGCTCGA
AAGCCGCATAG

Upstream 100 bases:

>100_bases
CTCGGTCGTCGAGAAGAATCTGCTAAAAGGCGCGACCGAACAATCACCCCTTTACAACTAACATGTCAGCCTGCTACTTC
TGACCCGTTAGCTGGGAGGA

Downstream 100 bases:

>100_bases
GTCGGGCATGTTTCTGGAGAAGGCGATGGACGGAACCGAGGAGCAGCCGACACTGCGGGAAAAGGCGTATGCGAGCTTTA
CGCGTCATCTTCTTGCACGT

Product: dihydrodipicolinate synthase protein

Products: NA

Alternate protein names: DHDPS [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKLTSRFGLSVALATPFDSDGNIAIEAMIAQARRSLAAGCSSVTLFGTTGEGSSVGNGERERVLAAFLDAGIAPGNIIVG
VLVDAAEDAAMQAAQALSRGARNILLAPPSYFKNVSDDGVFQWFSNVFALLGDTARDIIVYNLPSLTMVPLSVSLIGRLR
TAFPHIVTGVKDSSGDWPYTEALLEAHSDLIILVGDERHLARAVRLGGQGAISGMANFVPHELKPMAEEGKDDSRVADFV
LELLKHPVVPAVKAMVARTTSEETWIAVRPPLTSISGEARAQLALAFDNLFRSKAA

Sequences:

>Translated_296_residues
MKLTSRFGLSVALATPFDSDGNIAIEAMIAQARRSLAAGCSSVTLFGTTGEGSSVGNGERERVLAAFLDAGIAPGNIIVG
VLVDAAEDAAMQAAQALSRGARNILLAPPSYFKNVSDDGVFQWFSNVFALLGDTARDIIVYNLPSLTMVPLSVSLIGRLR
TAFPHIVTGVKDSSGDWPYTEALLEAHSDLIILVGDERHLARAVRLGGQGAISGMANFVPHELKPMAEEGKDDSRVADFV
LELLKHPVVPAVKAMVARTTSEETWIAVRPPLTSISGEARAQLALAFDNLFRSKAA
>Mature_296_residues
MKLTSRFGLSVALATPFDSDGNIAIEAMIAQARRSLAAGCSSVTLFGTTGEGSSVGNGERERVLAAFLDAGIAPGNIIVG
VLVDAAEDAAMQAAQALSRGARNILLAPPSYFKNVSDDGVFQWFSNVFALLGDTARDIIVYNLPSLTMVPLSVSLIGRLR
TAFPHIVTGVKDSSGDWPYTEALLEAHSDLIILVGDERHLARAVRLGGQGAISGMANFVPHELKPMAEEGKDDSRVADFV
LELLKHPVVPAVKAMVARTTSEETWIAVRPPLTSISGEARAQLALAFDNLFRSKAA

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family [H]

Homologues:

Organism=Escherichia coli, GI1788823, Length=222, Percent_Identity=28.3783783783784, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786463, Length=274, Percent_Identity=24.0875912408759, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.2.1.52 [H]

Molecular weight: Translated: 31342; Mature: 31342

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTSRFGLSVALATPFDSDGNIAIEAMIAQARRSLAAGCSSVTLFGTTGEGSSVGNGER
CCCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHH
ERVLAAFLDAGIAPGNIIVGVLVDAAEDAAMQAAQALSRGARNILLAPPSYFKNVSDDGV
HHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCHH
FQWFSNVFALLGDTARDIIVYNLPSLTMVPLSVSLIGRLRTAFPHIVTGVKDSSGDWPYT
HHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EALLEAHSDLIILVGDERHLARAVRLGGQGAISGMANFVPHELKPMAEEGKDDSRVADFV
HHHHHCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHCCHHCCCHHHCCCCHHHHHHHH
LELLKHPVVPAVKAMVARTTSEETWIAVRPPLTSISGEARAQLALAFDNLFRSKAA
HHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLTSRFGLSVALATPFDSDGNIAIEAMIAQARRSLAAGCSSVTLFGTTGEGSSVGNGER
CCCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHH
ERVLAAFLDAGIAPGNIIVGVLVDAAEDAAMQAAQALSRGARNILLAPPSYFKNVSDDGV
HHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCHH
FQWFSNVFALLGDTARDIIVYNLPSLTMVPLSVSLIGRLRTAFPHIVTGVKDSSGDWPYT
HHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EALLEAHSDLIILVGDERHLARAVRLGGQGAISGMANFVPHELKPMAEEGKDDSRVADFV
HHHHHCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHCCHHCCCHHHCCCCHHHHHHHH
LELLKHPVVPAVKAMVARTTSEETWIAVRPPLTSISGEARAQLALAFDNLFRSKAA
HHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA