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The map label for this gene is panB

Identifier: 86360736

GI number: 86360736

Start: 1599

End: 2420

Strand: Direct

Name: panB

Synonym: RHE_PF00002

Alternate gene names: 86360736

Gene position: 1599-2420 (Clockwise)

Preceding gene: 86360735

Following gene: 86360738

Centisome position: 0.25

GC content: 63.02

Gene sequence:

>822_bases
ATGAGCGCCATCGGATCGCAGAAGCGCCTGACGCCGCCCCGCATCTCAGCCATGAAGGGCGGCAAACCGATTGTCTGCCT
GACCGCCTACACCACGCCGATGGCGCGACTTCTCGACGATCATTGCGACCTGCTGCTGGTCGGGGATTCGCTCGGCATGG
TGCTCTATGGCATGGAGACGACCATCGGGGTCACACTCGATATGATGATCGCCCACGGCAAGGCCGTGATGCGCGGCGTC
GCCAAGGCCTGCGTCGTCGTCGACATGCCGTTCGGCAGTTACCAGGAATCGAAAGAGGTTGCCTTCCGAAACGCCGTGCG
CATCCTGCAGGAAACCGGCTGCGACGCCGTGAAGCTGGAAGGCGGCGAGGAGATGGCCGAGACGATTGCCTTTCTGACGA
AGCGGGGGATCCCGGTCATGGGGCACATCGGGCTGATGCCGCAGCAGGTTCAGACTGCCGGCGGCTATCGTTCCGTTGGG
CACTCAGAACACGAGACGTCGAAAATCCGGCGCGACGCCCATGCCATCGGCGGCTCGGGAGCCTTCGCCGTCGTCATTGA
AGGCACCGTGGAACCGCTCGCGCGCGAGGTGACTTCAGCCATGCACATACCCACCATCGGCATCGGTGCCTCCGCCGCTT
GCGACGGGCAGGTTCTAGTCTCTGACGATATTCTGGGGCTCTTCAACGATTTCAAGCCGCGTTTCGTCAAGCGTTACGAC
GAACTCGGCAAGAGGGTCGCGGATGCCGTCGCCGCCTATGCAGAGGACGTGCGCTCGCGAAAGTTCCCGGCAGCCGACCA
TACGTTCAAGCGACGGCCGTAA

Upstream 100 bases:

>100_bases
TCTTTGTCCGGGTCGGCTCGACGCGCCTTCTCGATAACCGGGTGATCGGCGACAACAGAACGATCGGCGGGCGAAACCAG
CCGGGAAAGGGAGTGACGAA

Downstream 100 bases:

>100_bases
GACGAAGGCTCTGGCCGCTTATCGTTCCTCAACCCTTTCCCGCAAGGGAAGGTCTCGGGGCGCCAGCGGGCCAAGTCCGT
CACCCGCCGCCATACTGCTG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSAIGSQKRLTPPRISAMKGGKPIVCLTAYTTPMARLLDDHCDLLLVGDSLGMVLYGMETTIGVTLDMMIAHGKAVMRGV
AKACVVVDMPFGSYQESKEVAFRNAVRILQETGCDAVKLEGGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVG
HSEHETSKIRRDAHAIGGSGAFAVVIEGTVEPLAREVTSAMHIPTIGIGASAACDGQVLVSDDILGLFNDFKPRFVKRYD
ELGKRVADAVAAYAEDVRSRKFPAADHTFKRRP

Sequences:

>Translated_273_residues
MSAIGSQKRLTPPRISAMKGGKPIVCLTAYTTPMARLLDDHCDLLLVGDSLGMVLYGMETTIGVTLDMMIAHGKAVMRGV
AKACVVVDMPFGSYQESKEVAFRNAVRILQETGCDAVKLEGGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVG
HSEHETSKIRRDAHAIGGSGAFAVVIEGTVEPLAREVTSAMHIPTIGIGASAACDGQVLVSDDILGLFNDFKPRFVKRYD
ELGKRVADAVAAYAEDVRSRKFPAADHTFKRRP
>Mature_272_residues
SAIGSQKRLTPPRISAMKGGKPIVCLTAYTTPMARLLDDHCDLLLVGDSLGMVLYGMETTIGVTLDMMIAHGKAVMRGVA
KACVVVDMPFGSYQESKEVAFRNAVRILQETGCDAVKLEGGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVGH
SEHETSKIRRDAHAIGGSGAFAVVIEGTVEPLAREVTSAMHIPTIGIGASAACDGQVLVSDDILGLFNDFKPRFVKRYDE
LGKRVADAVAAYAEDVRSRKFPAADHTFKRRP

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=249, Percent_Identity=37.7510040160643, Blast_Score=168, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319653, Length=261, Percent_Identity=36.3984674329502, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_RHIEC (Q2JZU0)

Other databases:

- EMBL:   CP000138
- RefSeq:   YP_472623.1
- ProteinModelPortal:   Q2JZU0
- SMR:   Q2JZU0
- STRING:   Q2JZU0
- GeneID:   3896083
- GenomeReviews:   CP000138_GR
- KEGG:   ret:RHE_PF00002
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29320; Mature: 29189

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 88-88 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIGSQKRLTPPRISAMKGGKPIVCLTAYTTPMARLLDDHCDLLLVGDSLGMVLYGMET
CCCCCCCCCCCCCCHHHCCCCCCEEEEEECHHHHHHHHHHCCCEEEECCCCCEEEEECHH
TIGVTLDMMIAHGKAVMRGVAKACVVVDMPFGSYQESKEVAFRNAVRILQETGCDAVKLE
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
GGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVGHSEHETSKIRRDAHAIGGSG
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
AFAVVIEGTVEPLAREVTSAMHIPTIGIGASAACDGQVLVSDDILGLFNDFKPRFVKRYD
EEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCHHHHHHHH
ELGKRVADAVAAYAEDVRSRKFPAADHTFKRRP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAIGSQKRLTPPRISAMKGGKPIVCLTAYTTPMARLLDDHCDLLLVGDSLGMVLYGMET
CCCCCCCCCCCCCHHHCCCCCCEEEEEECHHHHHHHHHHCCCEEEECCCCCEEEEECHH
TIGVTLDMMIAHGKAVMRGVAKACVVVDMPFGSYQESKEVAFRNAVRILQETGCDAVKLE
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
GGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVGHSEHETSKIRRDAHAIGGSG
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
AFAVVIEGTVEPLAREVTSAMHIPTIGIGASAACDGQVLVSDDILGLFNDFKPRFVKRYD
EEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCHHHHHHHH
ELGKRVADAVAAYAEDVRSRKFPAADHTFKRRP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA