Click here to switch to the map view.

The map label for this gene is vsr [H]

Identifier: 162329649

GI number: 162329649

Start: 119557

End: 120039

Strand: Direct

Name: vsr [H]

Synonym: RHE_PF00110

Alternate gene names: 162329649

Gene position: 119557-120039 (Clockwise)

Preceding gene: 86360842

Following gene: 86360844

Centisome position: 18.61

GC content: 58.8

Gene sequence:

>483_bases
ATGGCCGATAGACTTACCCCGGAGCAGCGCCGGCTCAATATGAGCCGCATCCGCGCTAAGAATACAGCGCCAGAATGGAT
AATACGTCGGCTTCTGCACAGCCGAGGTTTCCGATACCAGCTTCATCGAAGGGACCTGCCGGGCAAGCCGGATTTAGTTT
TCCCACGGTTTCGCACGGCGGTCTTCGTGCATGGCTGTTTCTGGCATGGTCACGCCTGCTCGCTGTTTCGTATGCCGGCA
ACTCGCACGGAGTTTTGGTCAGCTAAGATCGGGGTCAACCGTGAGCGCGATGCCGCTGCAATGATCTCCCTCCAAGATCT
CGGCTGGCGCAGCCTGTGGATTTGGGAATGCGCGCTCAAGGGCCGCGGCCGCCTGCCGGAAAGCCAGCTCGCGGAGCAGG
TTACAGCCTTCCTGAACAGCGGCGCCATATTCGACGAGATTGCGGAGAACCGTATTTCACCCACAGCGGAGGAGATTTTA
TGA

Upstream 100 bases:

>100_bases
GCGCTCTCAGCTGCCTTTCCTGCCGTGCCAGCTGATCTGGCGATCGGCGCGCCTCTGGTTGAGGATGATGCCGTGCCCCC
GGAGCAGGCGGCTGGGAGCG

Downstream 100 bases:

>100_bases
GCCAACCCCCGCCAGCTCTGTCTGCAGATCCATCGGATGATTTTGCTAGCCTGAGCGACATTGCGCTGCTTCGACTGCGC
GGCAGGGTCGCGGCCGAACT

Product: DNA mismatch endonuclease protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MADRLTPEQRRLNMSRIRAKNTAPEWIIRRLLHSRGFRYQLHRRDLPGKPDLVFPRFRTAVFVHGCFWHGHACSLFRMPA
TRTEFWSAKIGVNRERDAAAMISLQDLGWRSLWIWECALKGRGRLPESQLAEQVTAFLNSGAIFDEIAENRISPTAEEIL

Sequences:

>Translated_160_residues
MADRLTPEQRRLNMSRIRAKNTAPEWIIRRLLHSRGFRYQLHRRDLPGKPDLVFPRFRTAVFVHGCFWHGHACSLFRMPA
TRTEFWSAKIGVNRERDAAAMISLQDLGWRSLWIWECALKGRGRLPESQLAEQVTAFLNSGAIFDEIAENRISPTAEEIL
>Mature_159_residues
ADRLTPEQRRLNMSRIRAKNTAPEWIIRRLLHSRGFRYQLHRRDLPGKPDLVFPRFRTAVFVHGCFWHGHACSLFRMPAT
RTEFWSAKIGVNRERDAAAMISLQDLGWRSLWIWECALKGRGRLPESQLAEQVTAFLNSGAIFDEIAENRISPTAEEIL

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=138, Percent_Identity=45.6521739130435, Blast_Score=133, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 18615; Mature: 18484

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRLTPEQRRLNMSRIRAKNTAPEWIIRRLLHSRGFRYQLHRRDLPGKPDLVFPRFRTA
CCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCHHHH
VFVHGCFWHGHACSLFRMPATRTEFWSAKIGVNRERDAAAMISLQDLGWRSLWIWECALK
HEEHHHEECCCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHEEHHHCCCCEEEEEEEECC
GRGRLPESQLAEQVTAFLNSGAIFDEIAENRISPTAEEIL
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHC
>Mature Secondary Structure 
ADRLTPEQRRLNMSRIRAKNTAPEWIIRRLLHSRGFRYQLHRRDLPGKPDLVFPRFRTA
CCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCHHHH
VFVHGCFWHGHACSLFRMPATRTEFWSAKIGVNRERDAAAMISLQDLGWRSLWIWECALK
HEEHHHEECCCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHEEHHHCCCCEEEEEEEECC
GRGRLPESQLAEQVTAFLNSGAIFDEIAENRISPTAEEIL
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]