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The map label for this gene is tgt [H]

Identifier: 85860047

GI number: 85860047

Start: 2258633

End: 2259763

Strand: Direct

Name: tgt [H]

Synonym: SYN_00038

Alternate gene names: 85860047

Gene position: 2258633-2259763 (Clockwise)

Preceding gene: 85860046

Following gene: 85860048

Centisome position: 71.04

GC content: 54.38

Gene sequence:

>1131_bases
ATGCCTCCTTTAAAACAACATTTCAGGATACTGAATACTGACCCGGGATCGGCGGCACGGCTGGGTCTGCTGTCGACGGT
TCATGGCGAAGTCCACACCCCGGCATTCATGCCCGTGGGAACGCAGGGAACGGTTAAAGCCCTGACCCCGGACACGGTGA
AATCCCTGGGGGCGGAGATAATCCTCTGCAATACCTACCATCTTTATCTGAGACCCGGCCACCGGCTGATTGAGTCCCTG
GGCGGACTGCATCGCTTCATGAACTGGCCGGGCCCTATCTTAACGGACAGCGGTGGCTTTCAGGTTTACAGTCTGGGAAA
ACTGCGGAAAGTAACCCGGGAAGGGGTGATTTTTCAATCCCATATCGACGGCTCCCGGCATAAAATCACCCCGGAACTTG
CCATTGAAATTCAGGAAGCCTTGAACACGGATATCATGATGTGTCTGGATGAATGCACCCCCTGGCCGGCCGGTTTTCAG
GAAGCGGAATCTTCCCTTTCCCTGACGCTGTCCTGGGCCGAGCGAAGCCGGAAGGCCAGGACTAATTCTTCTTCCCAAGC
CCTCTACGGAATCATTCAGGGAGGAATGTATCTGGAATTGAGAAGACGCGCCGTGGAAGCAATAACGGCTATGGATTTCG
ACGGCTATGCCCTGGGAGGAGTCAGTGTGGGCGAACCCAAACCCCTGATGGAACAGATCGTCAGTGAAACGGCTCCCCTT
CTTCCGGAGGATCGTCCTCATTATCTCATGGGTGTGGGAACGCCGGAGGATATCGTCCGGGCCGTGGACTGCGGAATCGA
TCTGTTCGACTGCGTCATGCCCACCCGATGCGCCAGGCACGGTTTATTGTTTACAAATGCTGAAAAGGTTGTTATAAAAA
ACGCCCGTTATCGGGAAGACAACGGGCCTCTGGACAGCGATTGCGATTGTTATACCTGCCGGAACTTCTCGCGGGCCTAC
CTGAGGCATTTATATGTGGCCGGCGAAATCCTGGCCATGACCTTGAACACTCTTCACAACATCCGCTATTATATGCGCCT
TATGGAGCAGATTCGGAGCGCCATAGGACGCGGTCAATACGCGGCTTTTAAAACCCGGTTTCTAAAAGACCGTCGAATGT
CGGAAGCCTGA

Upstream 100 bases:

>100_bases
GCGACGGCCATTCAGGAAGAATACCGCTTTTACAGTTACGGCGACTGCATGCTGATCATTTAGGCGGGCACGTTCCGAAA
AGGCTTATCCGGATAAACTT

Downstream 100 bases:

>100_bases
CTCCTGATCAGGGTATATTCATAATGTCATAATGGACACGATTGAAGGAGGATAACGCATTGACAGACTTAGCTTATGCC
ATGGGAAGCGCTCCCGGAGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MPPLKQHFRILNTDPGSAARLGLLSTVHGEVHTPAFMPVGTQGTVKALTPDTVKSLGAEIILCNTYHLYLRPGHRLIESL
GGLHRFMNWPGPILTDSGGFQVYSLGKLRKVTREGVIFQSHIDGSRHKITPELAIEIQEALNTDIMMCLDECTPWPAGFQ
EAESSLSLTLSWAERSRKARTNSSSQALYGIIQGGMYLELRRRAVEAITAMDFDGYALGGVSVGEPKPLMEQIVSETAPL
LPEDRPHYLMGVGTPEDIVRAVDCGIDLFDCVMPTRCARHGLLFTNAEKVVIKNARYREDNGPLDSDCDCYTCRNFSRAY
LRHLYVAGEILAMTLNTLHNIRYYMRLMEQIRSAIGRGQYAAFKTRFLKDRRMSEA

Sequences:

>Translated_376_residues
MPPLKQHFRILNTDPGSAARLGLLSTVHGEVHTPAFMPVGTQGTVKALTPDTVKSLGAEIILCNTYHLYLRPGHRLIESL
GGLHRFMNWPGPILTDSGGFQVYSLGKLRKVTREGVIFQSHIDGSRHKITPELAIEIQEALNTDIMMCLDECTPWPAGFQ
EAESSLSLTLSWAERSRKARTNSSSQALYGIIQGGMYLELRRRAVEAITAMDFDGYALGGVSVGEPKPLMEQIVSETAPL
LPEDRPHYLMGVGTPEDIVRAVDCGIDLFDCVMPTRCARHGLLFTNAEKVVIKNARYREDNGPLDSDCDCYTCRNFSRAY
LRHLYVAGEILAMTLNTLHNIRYYMRLMEQIRSAIGRGQYAAFKTRFLKDRRMSEA
>Mature_375_residues
PPLKQHFRILNTDPGSAARLGLLSTVHGEVHTPAFMPVGTQGTVKALTPDTVKSLGAEIILCNTYHLYLRPGHRLIESLG
GLHRFMNWPGPILTDSGGFQVYSLGKLRKVTREGVIFQSHIDGSRHKITPELAIEIQEALNTDIMMCLDECTPWPAGFQE
AESSLSLTLSWAERSRKARTNSSSQALYGIIQGGMYLELRRRAVEAITAMDFDGYALGGVSVGEPKPLMEQIVSETAPLL
PEDRPHYLMGVGTPEDIVRAVDCGIDLFDCVMPTRCARHGLLFTNAEKVVIKNARYREDNGPLDSDCDCYTCRNFSRAYL
RHLYVAGEILAMTLNTLHNIRYYMRLMEQIRSAIGRGQYAAFKTRFLKDRRMSEA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=42.3295454545455, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI13375872, Length=288, Percent_Identity=25, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786607, Length=361, Percent_Identity=53.7396121883656, Blast_Score=393, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17544680, Length=353, Percent_Identity=41.9263456090652, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI25146502, Length=226, Percent_Identity=26.5486725663717, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920510, Length=368, Percent_Identity=42.9347826086957, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI21355311, Length=160, Percent_Identity=31.875, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42024; Mature: 41893

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLKQHFRILNTDPGSAARLGLLSTVHGEVHTPAFMPVGTQGTVKALTPDTVKSLGAEI
CCCHHHHCEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCHHHHHHCCCEE
ILCNTYHLYLRPGHRLIESLGGLHRFMNWPGPILTDSGGFQVYSLGKLRKVTREGVIFQS
EEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECHHHHHHHHCCCEEEE
HIDGSRHKITPELAIEIQEALNTDIMMCLDECTPWPAGFQEAESSLSLTLSWAERSRKAR
CCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHCCCCEEEEEHHHHHHHHC
TNSSSQALYGIIQGGMYLELRRRAVEAITAMDFDGYALGGVSVGEPKPLMEQIVSETAPL
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCC
LPEDRPHYLMGVGTPEDIVRAVDCGIDLFDCVMPTRCARHGLLFTNAEKVVIKNARYRED
CCCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCEEEEECCEECCC
NGPLDSDCDCYTCRNFSRAYLRHLYVAGEILAMTLNTLHNIRYYMRLMEQIRSAIGRGQY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AAFKTRFLKDRRMSEA
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PPLKQHFRILNTDPGSAARLGLLSTVHGEVHTPAFMPVGTQGTVKALTPDTVKSLGAEI
CCHHHHCEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCHHHHHHCCCEE
ILCNTYHLYLRPGHRLIESLGGLHRFMNWPGPILTDSGGFQVYSLGKLRKVTREGVIFQS
EEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECHHHHHHHHCCCEEEE
HIDGSRHKITPELAIEIQEALNTDIMMCLDECTPWPAGFQEAESSLSLTLSWAERSRKAR
CCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHCCCCEEEEEHHHHHHHHC
TNSSSQALYGIIQGGMYLELRRRAVEAITAMDFDGYALGGVSVGEPKPLMEQIVSETAPL
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCC
LPEDRPHYLMGVGTPEDIVRAVDCGIDLFDCVMPTRCARHGLLFTNAEKVVIKNARYRED
CCCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCEEEEECCEECCC
NGPLDSDCDCYTCRNFSRAYLRHLYVAGEILAMTLNTLHNIRYYMRLMEQIRSAIGRGQY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AAFKTRFLKDRRMSEA
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]